Enzyme Polymorphisms as Genetic Signatures in Human Cell Cultures

O’Brien, Stephen J.; Kleiner, Gail; Olson, Russell; Shannon, John E.

doi:10.1126/science.841332

Cited by 90 publications

(35 citation statements)

References 31 publications

(30 reference statements)

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“…While this amount of perspective was greater than would have corresponded to the viewing distance between display and observer, we have found that such perspective is necessary for detecting the three-dimensional structure in these displays. 7 (8). The allozyme phenotype of the cultures was identical to that of HeLa cells for these enzymes and conforms to previously published results.…”

supporting

confidence: 77%

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T-1 Cells are HeLa and Not of Normal Human Kidney Origin

Nelson‐Rees¹,

Flandermeyer²,

Daniels³

1980

Science

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of results from strictly local mappings between neighboring points in successive frames.As a control experiment to verify that this superior discriminability of the coherent spherical pattern was associated with its three-dimensionality, we also examined performance under an analogous set of conditions with two unconnected but superimposed rectilinear plane patterns. The pattern of results for these planar patterns was very different, with no superiority in the detectability of the perfectly correlated pattern, no effect from small reductions in the correlation, and a competitive interference rather than global organization between two planes displaced in opposite directions.Thus, a single discrete projective transformation provides sufficient information for the detection of structure and motion in three dimensions. The underlying visual process is self-organizing, yielding a nonlinear stability sensitive to the global coherence of the changing optical pattern.

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supporting

confidence: 77%

“…The allozyme phenotype of the cultures was identical to that of HeLa cells for these enzymes and conforms to previously published results. The probability of a genotype at these eight loci in an individual cell line being identical to HeLa is .0017 (8).…”

mentioning

confidence: 99%

T-1 Cells are HeLa and Not of Normal Human Kidney Origin

Nelson‐Rees¹,

Flandermeyer²,

Daniels³

1980

Science

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“…The Results of experiments with HeLa marker enzymes and chromosomes were reproducibly confirmed (2-6) and also by another research group that examined the polymorphisms of several alloenzyme phenotypes as genetic signatures of cultured cells (7). Several years ago, human papillomavirus (HPV) 18 DNA was found to be integrated in the DNA of HeLa cells (8), and HPV 16 or 18 DNA was observed in other cervical cancer cell lines (9).…”

Section: Discussionmentioning

confidence: 64%

DETECTION OF HeLa CELL CONTAMINATION-PRESENCE OF HUMAN PAPILLOMA VIRUS 18 DNA AS HeLa MARKER IN JTC-3, OG AND OE CELL LINES

Ogura

Fujii

Hamano

et al. 1997

JJMSB

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SUMMARY:There are warnings of the contamination of cell cultures with HeLa cells in many laboratories in the world.The cell lines JTC-3 , OG and OE that were estabilished in Okayama in 1959, 1969 and 1971, respectively, were examined for human papillomavirus (HPV) 18 DNA by Southern blot hybridization. The HPV 18 DNA detected in these three cell lines showed hybridization patterns characteristic of the HPV 18 DNA in the HeLa cell line established in 1951. Southern hybridizaiton patterns of HPV 18 DNA in the cellular DNA of the C4-II cervical cancer cell line that was established in the USA in 1962 was different from that of HeLa cells. These results suggest that the JTC-3, OG and OE cell lines have been contaminated by HeLa cells.

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“…Isoenzyme polymorphism occurs among species, and sometimes even among breeds, individuals and tissues within a species [25]. of isoenzymes is currently the standard method for the quality control of cell line identification and detection of interspecies contamination.…”

Section: Isoenzyme Analysis Of Large Yorkshire Pig Fibroblastsmentioning

confidence: 99%