DETECTION OF HeLa CELL CONTAMINATION-PRESENCE OF HUMAN PAPILLOMA VIRUS 18 DNA AS HeLa MARKER IN JTC-3, OG AND OE CELL LINES

Ogura, Hajime; Fujii, Ritsushi; Hamano, Masako; Kuzuya, Mitsutaka; Nakajima, Hiroshi; Ohata, Ritsuko; Mori, Tadashige

doi:10.7883/yoken1952.50.161

Cited by 7 publications

(1 citation statement)

References 12 publications

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“…These were submitted by 27 of 93 scientists (29%) who made submissions . Van Helden (1988) reported that the human esophageal squamous carcinoma cell lines HCu 10, 18, 22, 27, and 39 were genetically identical, while Ogura et al (1997) reported that lines JTC-3, OF, and OE isolated in 1959, 1969, and 1971, respectively, were HeLa cells. More recently, Melcher (2005) reported that the putative normal colon epithelial cell line NCOL-1 was probably derived from the colon carcinoma LoVo.…”

Section: Brief Historymentioning

confidence: 99%

Eradication of cross-contaminated cell lines: A call for action

Nardone

2007

Cell Biol Toxicol

107

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This "white paper" was prepared and widely disseminated in an attempt to sound an alarm about the long-term existence of a grave, unresolved and growing problem that affects a significant portion of biomedical research, namely, the use of misidentified and cross-contaminated cell cultures. The "white paper" shows how bold action could bring about a profession-wide change in practice that would prevent further erosion. Misidentification and inter- and intra-specific cross-contamination of mammalian cell cultures used in research continues as a widespread problem despite an awareness that dates back more than 45 years. Awareness of the problem has led to a good understanding of the causes of cross-contamination and appropriate preventive measures. It has also led to the application of robust methods for the authentication of cell lines. Yet the problem continues unabated. Estimates of the incidence of research papers flawed by the use of misidentified and cross-contaminated cell cultures approximate 15-20%. The gravity of the situation calls for a strategy that would deliver a remedial message of authentication to virtually all cell culture researchers and also ensure compliance with the message. At the core of the strategy proposed herein is having cell line authentication as a condition for the award of research grants and for the publication of research findings.

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Section: Brief Historymentioning

confidence: 99%

Eradication of cross-contaminated cell lines: A call for action

Nardone

2007

Cell Biol Toxicol

107

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Basic Tissue Culture in Cancer Research

Cavanaugh

2005

The Cancer Handbook

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Basic Tissue and Cell Culture in Cancer Research

Cavanaugh

Haier

2007

The Cancer Handbook

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Cell culture techniques are the vital basis for the large majority of experimental cancer research. During the last 50 years, a long list of standard procedures and formulas have been developed for cell cultures. Almost all malignant tumour entities were established as immortal cell lines, and many of them are available commercially. The reproducibility and reliability of these cellular systems, however, is highly influenced by cell and tissue culture conditions, and many cellular functions can be modulated purely by altering the conditions of the culture. This chapter provides an introduction into basic principles of tissue and cell culture, which are very important but not limited to cancer research.

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DETECTION OF HeLa CELL CONTAMINATION-PRESENCE OF HUMAN PAPILLOMA VIRUS 18 DNA AS HeLa MARKER IN JTC-3, OG AND OE CELL LINES

Cited by 7 publications

References 12 publications

Eradication of cross-contaminated cell lines: A call for action

Eradication of cross-contaminated cell lines: A call for action

Basic Tissue Culture in Cancer Research

Basic Tissue and Cell Culture in Cancer Research

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