Enzyme mediated assembly of gold nanoparticles for ultrasensitive colorimetric detection of hepatitis C virus antibody

Cheng, Yu-Hong; Tang, Hao; Jiang, Jian‐Hui

doi:10.1039/c7ay01086a

Cited by 11 publications

(4 citation statements)

References 28 publications

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“…As summarized in Table 1, many molecular and serological assays have been demonstrated for HCV detection over the years. 7,14,[40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58] Different sensing methodologies were utilized for this purpose, such as ELISA, gold nanoparticles and quantum dots. Although these methods provide LoD values as low as 0.1 pg mL −1 , the requirement of laboratory-based spectrophotometers or confocal microscopy increases the cost and limits the applicability.…”

Section: Validation Of the µPisa Platformmentioning

confidence: 99%

Fabrication and development of a microfluidic paper-based immunosorbent assay platform (μPISA) for colorimetric detection of hepatitis C

Ozefe

Yıldız

2023

Analyst

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Section: Validation Of the µPisa Platformmentioning

confidence: 99%

Fabrication and development of a microfluidic paper-based immunosorbent assay platform (μPISA) for colorimetric detection of hepatitis C

Ozefe

Yıldız

2023

Analyst

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“…Hepatitis C virus antibody (anti-HCV) is also detected by unmodified AuNP aggregation induced by acetylcholinesterasecatalyzed hydrolysis reaction. The color of AuNP can be observed by naked eye at a detection limit of 10 -13 g/mL anti-HCV [130]. Furthermore, unmodified AuNP was demonstrated for dengue virus detection based on peptide nucleic acid (PNA)/DNA hybridization [131].…”

Section: Unmodified Gold Nanoparticles (Aunps)mentioning

confidence: 99%

Colorimetric biosensors for point-of-care virus detections

Zhao

Wong

Zheng

et al. 2020

Materials Science for Energy Technologies

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a b s t r a c tColorimetric biosensors can be used to detect a particular analyte through color changes easily by naked eyes or simple portable optical detectors for quantitative measurement. Thus, it is highly attractive for point-of-care detections of harmful viruses to prevent potential pandemic outbreak, as antiviral medication must be administered in a timely fashion. This review paper summaries existing and emerging techniques that can be employed to detect viruses through colorimetric assay design with detailed discussion of their sensing principles, performances as well as pros and cons, with an aim to provide guideline on the selection of suitable colorimetric biosensors for detecting different species of viruses. Among the colorimetric methods for virus detections, loop-mediated isothermal amplification (LAMP) method is more favourable for its faster detection, high efficiency, cheaper cost, and more reliable with high reproducible assay results. Nanoparticle-based colorimetric biosensors, on the other hand, are most suitable to be fabricated into lateral flow or lab-on-a-chip devices, and can be coupled with LAMP or portable PCR systems for highly sensitive on-site detection of viruses, which is very critical for early diagnosis of virus infections and to prevent outbreak in a swift and controlled manner.

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“…Recently, Cheng et al developed a technique that allows the enzyme-mediated assembly of GNPs for the colorimetric detection of the anti-HCV antibody, as shown in Figure 10 [126].…”

Section: Nanotechnologymentioning

confidence: 99%

Developments in the HCV Screening Technologies Based on the Detection of Antigens and Antibodies

Warkad

Song

Pal

et al. 2019

Sensors

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Hepatitis C virus (HCV) accounts for 15%–20% of cases of acute infection, and chronic HCV infection is developed in about 50%–80% of HCV patients. Unfortunately, due to the lack of proper medical care, difficulty in screening for HCV infection, and lack of awareness resulted in chronic HCV infection in 71 million people on a global scale, and about 399,000 deaths in 2016. It is crucial to recognize that the effective use of antiviral medicines can cure more than 95% of HCV infected people. The Global Health Sector Strategy (GHSS) aim is to reduce the new HCV infections and the HCV associated mortality by 90% and 65%, respectively. Therefore, the methods that are simple, yet powerful enough to detect HCV infections with high sensitivity, specificity, and a shorter window period are crucial to restrain the global burden of HCV healthcare. This article focuses on the technologies used for the detection of HCV in clinical specimens.

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Enzyme mediated assembly of gold nanoparticles for ultrasensitive colorimetric detection of hepatitis C virus antibody

Abstract: A novel enzyme mediated AuNP assembly based colorimetric method for ultrasensitive detection of hepatitis C virus antibody.

Cited by 11 publications

References 28 publications

Fabrication and development of a microfluidic paper-based immunosorbent assay platform (μPISA) for colorimetric detection of hepatitis C

Fabrication and development of a microfluidic paper-based immunosorbent assay platform (μPISA) for colorimetric detection of hepatitis C

Colorimetric biosensors for point-of-care virus detections

Developments in the HCV Screening Technologies Based on the Detection of Antigens and Antibodies

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