Enzyme Leakage as an Indicator of Cytotoxicity in Cultured Cells

Welder, Allison A.; Acosta, Daniel

doi:10.1016/b978-0-08-092440-3.50010-5

Cited by 27 publications

(12 citation statements)

References 7 publications

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“…Cell toxicity was estimated from the ratio between lactate dehydrogenase (LDH) activity in the culture medium and that of the whole cell content (Welder & Acosta, 1994). Cells were plated at a density of 6.75 × 10 5 cells per plate (60 mm diameter plates); after incubation, the culture medium was collected, cells were scraped in 3 mL PBS and sonicated to disrupt cell membrane and release the total amount of LDH.…”

Section: Cell Viability and Cytotoxicitymentioning

confidence: 99%

Dihydrocaffeic acid, a major microbial metabolite of chlorogenic acids, shows similar protective effect than a yerba mate phenolic extract against oxidative stress in HepG2 cells

Baeza

Sarriá

Mateos

et al. 2016

Food Research International

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Section: Cell Viability and Cytotoxicitymentioning

confidence: 99%

Dihydrocaffeic acid, a major microbial metabolite of chlorogenic acids, shows similar protective effect than a yerba mate phenolic extract against oxidative stress in HepG2 cells

Baeza

Sarriá

Mateos

et al. 2016

Food Research International

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“…To each well containing the sample, 200 L of a mixture comprising 5 mM piruvate, 0.35 mM NADH, and 84 mM Tris was added and immediately read at 340 nm for 3 min in a microplate reader. LDH leakage was estimated from the ratio between the LDH activity in the culture medium and that of the whole cell content [26,27].…”

Section: Cytotoxicity Assay (Lactate Dehydrogenase Leakage Assay)mentioning

confidence: 99%

Response of the antioxidant defense system to tert‐butyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2)

Alía

Ramos

Mateos

et al. 2005

J Biochem & Molecular Tox

211

195

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The aim of this work was to investigate the response of the antioxidant defense system to two oxidative stressors, hydrogen peroxide and tert‐butyl hydroperoxide, in HepG2 cells in culture. The parameters evaluated included enzyme activity and gene expression of superoxide dismutase, catalase, glutathione peroxidase, and activity of glutathione reductase. Besides, markers of the cell damage and oxidative stress evoked by the stressors such as cell viability, intracellular reactive oxygen species generation, malondialdehyde levels, and reduced glutathione concentration were evaluated. Both stressors, hydrogen peroxide and tert‐butyl hydroperoxide, enhanced cell damage and reactive oxygen species generation at doses above 50 μM. The concentration of reduced glutathione decreased, and levels of malondialdehyde and activity of the antioxidant enzymes consistently increased only when HepG2 cells were treated with tert‐butyl hydroperoxide but not when hydrogen peroxide was used. A slight increase in the gene expression of Cu/Zn superoxide dismutase and catalase with 500 μM tert‐butyl hydroperoxide and of catalase with 200 μ hydrogen peroxide was observed. The response of the components of the antioxidant defense system evaluated in this study indicates that tert‐butyl hydroperoxide evokes a consistent cellular stress in HepG2. © 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:119–128, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20061

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“…LDH leakage can be estimated from the ratio between the LDH activity in the culture medium and that of the whole cell content [50,51].…”

Section: 1) Cell Viabilitymentioning

confidence: 99%

A Cell Culture Model for the Assessment of the Chemopreventive Potential of Dietary Compounds.

Goya¹,

Martín²,

Ramos³

et al. 2009

CNF

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Enzyme Leakage as an Indicator of Cytotoxicity in Cultured Cells

Cited by 27 publications

References 7 publications

Dihydrocaffeic acid, a major microbial metabolite of chlorogenic acids, shows similar protective effect than a yerba mate phenolic extract against oxidative stress in HepG2 cells

Dihydrocaffeic acid, a major microbial metabolite of chlorogenic acids, shows similar protective effect than a yerba mate phenolic extract against oxidative stress in HepG2 cells

Response of the antioxidant defense system to tert‐butyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2)

A Cell Culture Model for the Assessment of the Chemopreventive Potential of Dietary Compounds.

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