Enzyme Immobilization on Layered and Nanostructured Materials

Pavlidis, Ioannis V.; Tzialla, Aikaterini A.; Enotiadis, Apostolos; Stamatis, Haralambos; Gournis, Dimitrios

doi:10.1002/9783527632534.ch2

Cited by 10 publications

(6 citation statements)

References 197 publications

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“…Recently, the development of nanostructured materials science resulted in a range of nanomaterials with different sizes and shapes, some of which are already used as immobilization matrices (Kim et al, 2008;Pavlidis et al, 2010c). Enzyme immobilization on nanostructured materials presents some advantages over the bulk solid materials, namely the high surface area which can lead to higher enzyme loading, the nanoscale dispersion and the ease of surface functionalization (Kim et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Development of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterials

Pavlidis

Vorhaben

Tsoufis

et al. 2012

Bioresource Technology

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138

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Development of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterials Pavlidis, Ioannis V.; Vorhaben, Torge; Tsoufis, Theodoros; Rudolf, Petra; Bornscheuer, Uwe T.; Gournis, Dimitrios; Stamatis, Haralambos Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. a b s t r a c tIn this study we report the use of functionalized carbon-based nanomaterials, such as amine-functionalized graphene oxide (GO) and multi-walled carbon nanotubes (CNTs), as effective immobilization supports for various lipases and esterases of industrial interest. Structural and biochemical characterization have revealed that the curvature of the nanomaterial affect the immobilization yield, the catalytic behavior and the secondary structure of enzymes. Infrared spectroscopy study indicates that the catalytic behavior of the immobilized enzymes is correlated with their a-helical content. Hydrolases exhibit higher esterification activity (up to 20-fold) when immobilized on CNTs compared to GO. The covalently immobilized enzymes exhibited comparable or even higher activity compared to the physically adsorbed ones, while they presented higher operational stability. The enhanced catalytic behavior observed for most of the hydrolases covalently immobilized on amine-functionalized CNTs indicate that these functionalized nanomaterials are suitable for the development of efficient nanobiocatalytic systems.

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Section: Introductionmentioning

confidence: 99%

Development of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterials

Pavlidis

Vorhaben

Tsoufis

et al. 2012

Bioresource Technology

Self Cite

138

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“…There are also frequent disadvantages, however, including difficult enzyme recovery, low product concentration, low productivity due to substrate and/or product inhibition and, hence, high recovery costs [34]. An important route to improving the performance of enzymes in non-natural environments and their ability to work in continuous processes is to immobilize them by either adsorption, covalent attachment or by incorporation in hydrophobic organic-inorganic hybrid materials [35][36][37][38].…”

Section: Biocatalysismentioning

confidence: 99%

Conversion Technologies for the Production of Liquid Fuels and Biochemicals

Dobbelaere¹,

Anthonis²,

Soetaert³

2014

Cellulosic Energy Cropping Systems

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“…They present excellent properties such as cation exchange capacity, porosity, swelling response and intercalation capacity [21,22], which make them valuable nanostructures for applications in catalysis, organic synthesis, and adsorption of inorganic/organic components, such as pollutants and dyes [21,[23][24][25]. The nature of the organized structure between the aluminosilicate sheets, as well as their tailor-made properties using simple techniques, may result in the controlled intercalation of molecules, and thus making them versatile supports for the specific immobilization of a plethora of biomolecules, such as enzymes [26]. The chemical and structural properties of layered smectite clays could be tailored and further improved by the addition of organic or polymeric molecules [27].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Laccase on Hybrid Super-Structured Nanomaterials for the Decolorization of Phenolic Dyes

et al. 2022

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In the present work, hybrid super-structured nanomaterials were synthesized by the combination of smectite nanoclays with various carbon-based nanomaterials (graphene oxide, carbon nanotubes and adamantylamine) and were used as nanosupports for the covalent and non-covalent immobilization of laccase from Trametes versicolor (TvL). TvL was successfully immobilized on these hybrid nanomaterials, achieving high immobilization yields (up to 85%), while its conformation remained unaltered upon immobilization. The apparent kinetic constants Vmax and Km of the immobilized enzymes strongly depended on the immobilization procedure and the composition of hybrid nanomaterials. Immobilized TvL preserved up to 50% of its initial activity after 24 h of incubation at 60 °C, while free enzyme was totally deactivated. The TvL-hybrid nanomaterials bioconjugates were efficiently applied for the degradation of various synthetic dyes, exhibiting excellent decolorization capacity, as well as high reusability (up to 11 successive catalytic cycles), providing insights into the use of these bionanoconjugates on applications with environmental, and industrial interest.

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Enzyme Immobilization on Layered and Nanostructured Materials

Cited by 10 publications

References 197 publications

Development of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterials

Development of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterials

Conversion Technologies for the Production of Liquid Fuels and Biochemicals

Immobilization of Laccase on Hybrid Super-Structured Nanomaterials for the Decolorization of Phenolic Dyes

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