Enzyme-based choline and l-glutamate biosensor electrodes on silicon microprobe arrays

Frey, Olivier; Holtzman, Tahl; McNamara, Ruth; Theobald, David E.; Wal, P. D. van der; Rooij, N. F. de; Dalley, Jeffrey W.; Koudelka‐Hep, M.

doi:10.1016/j.bios.2010.07.073

Cited by 57 publications

(80 citation statements)

References 64 publications

(72 reference statements)

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“…So it is very necessary to develop a rapid and sensitive method to detect L-glutamate in biological and food samples. So far, much efforts have been devoted to develop detection methods of L-glutamate, such as high performance liquid chromatography (HPLC) [20], capillary electrophoresis [21,22], optical methods [23][24][25] and electrochemical analyses [26,27]. Among these approaches, electrochemical methods, especially electrochemical biosensors, have attracted much attention due to their high sensitivity, rapid response and easy operation [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Amperometric l-glutamate biosensor based on bacterial cell-surface displayed glutamate dehydrogenase

Liang

Zhang

Lang

et al. 2015

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 99%

Amperometric l-glutamate biosensor based on bacterial cell-surface displayed glutamate dehydrogenase

Liang

Zhang

Lang

et al. 2015

Analytica Chimica Acta

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“…In this approach, the microelectrode arrangement can be flexibly designed and precisely controlled for use in dual-mode in vivo recording [30][31][32][33] . We modified the MEA probe with Pt nanoparticles and 1,3-phenylenediamine (mPD) to improve electrical performance.…”

Section: Introductionmentioning

confidence: 99%

An implantable microelectrode array for simultaneous L-glutamate and electrophysiological recordings in vivo

et al. 2015

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L-glutamate, the most common excitatory neurotransmitter in the mammalian central nervous system (CNS), is associated with a wide range of neurological diseases. Because neurons in CNS communicate with each other both electrically and chemically, dualmode (electric and chemical) analytical techniques with high spatiotemporal resolution are required to better understand glutamate function in vivo. In the present study, a silicon-based implantable microelectrode array (MEA) composed of both platinum electrochemical and electrophysiological microelectrodes was fabricated using micro-electromechanical system. In the MEA probe, the electrophysiological electrodes have a low impedance of 0.018 MΩ at 1 kHz, and the electrochemical electrodes show a sensitivity of 56 pA µM −1 to glutamate and have a detection limit of 0.5 µM. The MEA probe was used to monitor extracellular glutamate levels, spikes and local field potentials (LFPs) in the striatum of anaesthetised rats. To explore the potential of the MEA probe, the rats were administered to KCl via intraperitoneal injection. K + significantly increases extracellular glutamate levels, LFP low-beta range (12-18 Hz) power and spike firing rates with a similar temporal profile, indicating that the MEA probe is capable of detecting dual-mode neuronal signals. It was concluded that the MEA probe can help reveal mechanisms of neural physiology and pathology in vivo.

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“…Small joints are retaining the microprobe combs in the wafer grid, where they are stored and can then be released immediately before use (for further details and figures of each individual step, refer to ref. [17]). …”

Section: Silicon Microprobesmentioning

confidence: 99%

“…We recently developed a biosensor microprobe array [17], based on silicon microfabrication, and the corresponding array-compatible electrode functionalization techniques through electrochemical methods. Our next step included combining this array with a CMOS Figure 1 Layout of the microprobe comb consisting of four identical shanks of 6.5 mm length, each comprising two recessed 50 μm × 150 μm working electrodes (zoomed view) and one 40 μm × 500 μm electrode, used either as reference or counter electrode.…”

Section: Introductionmentioning

confidence: 99%

“…Although wire microelectrodes with a single recording site at the tip still may constitute the dominant experimental method for electrophysiological recordings [11], several groups start to employ microfabricated probe technology, in which multiple microelectrodes can be integrated on a single probe shank that then access multiple brain sites in parallel at relatively low tissue damage [5,9,14,17,30]. Arrays of such microprobes and electrodes can be precisely arranged in two or three dimensions, enabling a spatially highly resolved mapping of in vivo neural circuitry and interpretation of network-related phenomena [1,2,8,23,26,32].…”

Section: Introductionmentioning

confidence: 99%

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Multisite monitoring of choline using biosensor microprobe arrays in combination with CMOS circuitry

Frey¹,

Rothe²,

Heer³

et al. 2014

Biomedical Engineering / Biomedizinische Technik

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A miniature device enabling parallel in vivo detection of the neurotransmitter choline in multiple brain regions of freely behaving rodents is presented. This is achieved by combining a biosensor microprobe array with a custom-developed CMOS chip. Each silicon microprobe comprises multiple platinum electrodes that are coated with an enzymatic membrane and a permselective layer for selective detection of choline. The biosensors, based on the principle of amperometric detection, exhibit a sensitivity of 157 ± 35 µA mM -1 cm -2 , a limit of detection of below 1 µM, and a response time in the range of 1 s. With on-chip digitalization and multiplexing, parallel recordings can be performed at a high signal-to-noise ratio with minimal space requirements and with substantial reduction of external signal interference. The layout of the integrated circuitry allows for versatile configuration of the current range and can, therefore, also be used for functionalization of the electrodes before use. The result is a compact, highly integrated system, very convenient for on-site measurements.

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Enzyme-based choline and l-glutamate biosensor electrodes on silicon microprobe arrays

Cited by 57 publications

References 64 publications

Amperometric l-glutamate biosensor based on bacterial cell-surface displayed glutamate dehydrogenase

Amperometric l-glutamate biosensor based on bacterial cell-surface displayed glutamate dehydrogenase

An implantable microelectrode array for simultaneous L-glutamate and electrophysiological recordings in vivo

Multisite monitoring of choline using biosensor microprobe arrays in combination with CMOS circuitry

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