Amperometric l-glutamate biosensor based on bacterial cell-surface displayed glutamate dehydrogenase

An electrochemical sensor device for measuring blood ammonia at the point of care

“…However, as measurements are made in the gas phase, interfering species must also be volatilisable and be able to traverse the membrane. To compare the current device with other electrochemical sensors, its response to the presence of a number of common potential interferents was evaluated at typical concentrations [16][17][18][19][20]. Acetaminophen (600 µM) and ascorbic acid (100 µM) were assessed, along with related nitrogenous and ammoniacal compounds such as creatinine (45 µM), glutamic acid (100 µM) and uric acid (500 µM), and compared with impedimetric responses in PBS and 30 µM ammonia.…”

A Simple Flow Amperometric Electrochemical Biosensor Based on Chitosan Scaffolds and Gold Nanowires Modified on a Glassy Carbon Electrode for Detection of Glutamate in Food Products

“…The comparison of figure of merits of our system with previous reports is shown in Table . This biosensor has a detection limit (1.2 µM) at the same concentration as previous reports that modified CNT with another material, such as with CHIT‐CNT‐AuNP , CNT‐CHIT‐MB , Nafion‐bacteria‐PEI‐CNT . However, this biosensor demonstrated a wider linearity with four orders of magnitude (5 µM–20 mM) compared to the single CNT modification (two to three orders).…”

Comparison of NAD with NADP‐dependent Glutamate Dehydrogenase, and CNT with rGO‐modified Electrodes, for the Construction of Glutamate Sensors

“…There are two types of GDH enzymes and they require either nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP) as a coenzyme for their enzymatic activity. Glutamate sensors using separately NAD‐dependent GDH (GDH NAD ) and NADP‐dependent GDH (GDH NAD ) have previously been reported . It should be possible to determine glutamate levels from the anodic oxidation current of NADH and NADPH generated by the enzymatic reaction of GDH.…”

“…It should be possible to determine glutamate levels from the anodic oxidation current of NADH and NADPH generated by the enzymatic reaction of GDH. NADH and NADPH require a high electrical potential for electrolytic oxidation and thus carbon nanotube (CNT) have been used to improve the oxidation capacity of electrodes . The use of CNT‐modified electrodes provides high current response at lower potential compared to other electrode types.…”