Enzyme activity and genetic polymorphisms in patients with type II diabetes mellitus

Arpacı, Abdullah; Yalın, Serap; Ecevit, Hasret; Çömelekoğlu, Ülkü; Mete, Türkan

doi:10.17219/acem/125397

Cited by 6 publications

(3 citation statements)

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“…Whereas, the mean levels of CAT and SOD in DM patients are not significantly lower compared to the healthy group. These results are consistent with some previous report investigations which exhibited that the serum levels CAT, GSH, SOD, and GSH-Px are lower in DM patients as compared to the control group (Arpaci et al, 2020, Dworzański et al, 2020, Arribas et al, 2016. However, the results of this study are in disagreement with the results of a previous study that demonstrated the CAT level in serum DM patients is significantly higher than in the healthy group (Zarei et al, 2018).…”

Section: Resultssupporting

confidence: 87%

Evaluation of Advanced Glycation End Products, Oxidative Stress and Antioxidants and their Relationship in Diabetic patients

2023

ZJPAS

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Objective: Diabetes Mellitus (DM) is a major public health problem in developed countries; which also plays a vital role in providing information about advanced glycation end products (AGEs) and these induce oxidative stress (OS). The aim of this study is to evaluate the AGEs, OS, antioxidant, and lipid profile parameters in sera of DM patients. Methods: The current study includes 82 subjects (50 patients with type II DM and 32 healthy subjects, men and women). Demographic factors and medication intake of every subject were obtained. The advanced glycation end products (AGEs), oxidative stress (OS), antioxidant, and lipid profile parameters were measured using a spectrometric method and ELISA test. Results:The present study elucidated that the serum levels of CML, CEL, Pyralline, 8-OHdG, and MDA were significantly increased in diabetic patients compared with the healthy group. The serum levels of GSH, NO, and GSH-Px were significantly decreased in patients with DM rather than in the healthy group. While the serum levels of CAT and SOD were non-significantly decreased in patients with DM. Conclusion: Meanwhile, the elevation of AGEs and OS are associated with DM patients. NO and CML could be potential biomarkers for DM.

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Section: Resultssupporting

confidence: 87%

Evaluation of Advanced Glycation End Products, Oxidative Stress and Antioxidants and their Relationship in Diabetic patients

2023

ZJPAS

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“…On the other hand, Apraci et al revealed that CAT activity was observed to be significantly lower in the TT genotype compared to CT genotype in the T2DM group. Moreover, CAT ( p = 0.004 for the CC genotype, p = 0.0001 for CT and TT genotypes, respectively) enzyme activity was observed to be significantly lower in the T2DM compared to the control group [ 56 ]. Góth et al further noted that patients with T2DM with the CC or CT genotypes of -262 C/T had decreased CAT and increased glucose and HbA1c levels.…”

Section: Discussionmentioning

confidence: 99%

Non-Alcoholic Fatty Liver Disease Is Associated with a Decreased Catalase (CAT) Level, CT Genotypes and the T Allele of the -262 C/T CAT Polymorphism

Kosmalski,

Szymczak-Pajor,

Drzewoski

et al. 2023

Cells

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Background: It is well known that oxidative stress plays an important role in the development of non-alcoholic fatty liver disease (NAFLD). It has been suggested that an insufficient antioxidant defense system composed of antioxidant enzymes, including catalase (CAT) and nonenzymatic molecules, is a key factor triggering oxidative damage in the progression of liver disease. Therefore, the aim of our study was to assess whether the level of CAT and -262 C/T polymorphism in the promoter of CAT (rs1001179) are associated with NAFLD. Methods: In total, 281 adults (152/129 female/male, aged 65.61 ± 10.44 years) were included in the study. The patients were assigned to an NAFLD group (n = 139) or a group without NAFLD (n = 142) based on the results of an ultrasound, the Hepatic Steatosis Index, and the Fatty Liver Index (FLI). CAT levels were determined using an ELISA test, and genomic DNA was extracted via the standard phenol/chloroform-based method and genotyped via RFLP-PCR. Results: The CAT level was decreased in NAFLD patients (p < 0.001), and an ROC analysis revealed that a CAT level lower than 473.55 U/L significantly increases the risk of NAFLD. In turn, genotyping showed that the CT genotype and the T allele of -262 C/T CAT polymorphism elevate the risk of NAFLD. The diminished CAT level in the NAFLD group correlated with increased FLI, waist circumference and female gender. Conclusion: The obtained results support observations that oxidative damage associated with NAFLD may be the result of a decreased CAT level as a part of the antioxidant defense system.

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“…CAT antioxidant enzyme activity was significantly lower in carriers of TT genotype compared with the CT genotype among diabetic patients. The activity of PON1 was lower in carriers of MM genotype comparing with carriers of LL genotype among diabetic patients and controls [ 184 ]. Myeloperoxidase (MPO) belongs to the heme peroxidase superfamily that produces various diffusible radical species and reactive oxidants to initiate peroxidation of lipids [ 185 – 187 ].…”

Section: Introductionmentioning

confidence: 99%

Single-nucleotide polymorphisms as important risk factors of diabetes among Middle East population

et al. 2022

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Diabetes is a chronic metabolic disorder that leads to the dysfunction of various tissues and organs, including eyes, kidneys, and cardiovascular system. According to the World Health Organization, diabetes prevalence is 8.8% globally among whom about 90% of cases are type 2 diabetes. There are not any significant clinical manifestations in the primary stages of diabetes. Therefore, screening can be an efficient way to reduce the diabetic complications. Over the recent decades, the prevalence of diabetes has increased alarmingly among the Middle East population, which has imposed exorbitant costs on the health care system in this region. Given that the genetic changes are among the important risk factors associated with predisposing people to diabetes, we examined the role of single-nucleotide polymorphisms (SNPs) in the pathogenesis of diabetes among Middle East population. In the present review, we assessed the molecular pathology of diabetes in the Middle East population that paves the way for introducing an efficient SNP-based diagnostic panel for diabetes screening among the Middle East population. Since, the Middle East has a population of 370 million people; the current review can be a reliable model for the introduction of SNP-based diagnostic panels in other populations and countries around the world.

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Enzyme activity and genetic polymorphisms in patients with type II diabetes mellitus

Cited by 6 publications

References 0 publications

Evaluation of Advanced Glycation End Products, Oxidative Stress and Antioxidants and their Relationship in Diabetic patients

Evaluation of Advanced Glycation End Products, Oxidative Stress and Antioxidants and their Relationship in Diabetic patients

Non-Alcoholic Fatty Liver Disease Is Associated with a Decreased Catalase (CAT) Level, CT Genotypes and the T Allele of the -262 C/T CAT Polymorphism

Single-nucleotide polymorphisms as important risk factors of diabetes among Middle East population

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