Enzymatic Hydrolysis of Porang by Streptomyces violascens BF 3.10 Mannanase for the Production of Mannooligosaccharides

Abstract

Porang (Amorphophallus muelleriBlumeo C dengan aktivitas enzim sebesar 16.38 U/mL dan stabil pada suhu 4 °C selama 48 jam. Selama 5 jam waktu hidrolisis, reaksi dengan konsentrasi substrat 0,25%, 0,5%, dan 1% glukomanan porang yang dilarutkan dalam 10 mL enzim menghasilkan manooligosakarida dengan derajat polimerisasi 2-3. Visualisasi produk hidrolisis mengggunakan metode thin layer chromatography (TLC) dan high performance liquid chromatography (HPLC) menunjukkan bahwa jenis manooligosakarida yang dihasilkan adalah glukosa, manobiosa, manotriosa, dan manotetrosa. Derajat polimerisasi dan gula-gula sederhana yang terbentuk menunjukkan bahwa mananase S. violascens bekerja secara aktif mengatalisis reaksi hidrolisis ikatan 1,4-β-D-manosida dari rantai utama β-1,4-mannan sehingga menghasilkan gula-gula sederhana manooligosakarida.

OLIGO-GLUCOMANNAN PRODUCTION FROM PORANG (Amorphophallus oncophyllus) GLUCOMANNAN BY ENZYMATIC HYDROLYSIS USING β-MANNANASE

“…According to Harmayani et al (2014), PGM flour contains glucomannan almost the same around 92.69% of glucomannan. Meanwhile, the glucomannan content in porang powder roughly of 75.72% (Safitri et al, 2014). As a result that PGM was consisted of lipid (2.52 g/100 g ±0.10), crude fiber (0.37 g/100 g ±0.05), protein (0.33 g/100 g ±0.08), ash (0.66 g/100 g ±0.02) and water (8.48 g/100 g ±0.15).…”

“…The reducing sugar of POG was obtained under optimal conditions (1:1000 of E/S, 5.5 pH, and 37 o C) is summarized in Figure 1. The amount of reducing sugar shows the ability of mannanase to hydrolyze porang glucomannan (Safitri et al, 2014). Accordingly, Chen et al (2013) reported that oligosaccharides production is influenced precisely by reducing sugar present.…”

“…More recent studies reported that DP of oligosaccharides produced by all kinds of substrates using enzyme mannanase indicated enzyme capability is restrict to only degrading substrate into oligosaccharide such as mannobiose and not further activity to get small compound (Safitri et al, 2014). Notably, Jian et al (2013) So, in the present study indicated that PGM was successfully hydrolyzed by using β-mannase produced DP of POG with lower chain length than its native which prepared by cabinet drying (DP 9.4) and by freeze-drying (DP 12) (Harmayani et al, 2014).…”

“…Porang is a parental plant in Indonesia which belongs to the genus Amorphophallus of the Araceae family. Its bulb has glucomannan rich up to 75.72% in porang flour (Safitri et al, 2014) and 90.98% in porang tuber (Yanuriati et al, 2017). Although, the high yield of glucomannan is obtained by extraction from dried porang tuber but for industrial scale production prefers extraction from fresh porang tuber due to no drying process is needed thus lower cost of production (Yanuriati et al, 2017).…”

Porang Glucomannan Supplementation Improves Lipid Profile in Metabolic Syndrome Induced Rats

“…12 . Glucomannan also potential as a source of monooligosaccharide prebiotics due to its modulating effect to the microflora in the gastrointestinal tract 13 . Glucomannan mechanism of the modulating effect has been shown to be similiar with other soluble fibers i.e., fermentable fiber.…”