Enzymatic hydrolysis of carotenoid esters of marigold flowers (Tagetes erecta L.) and red paprika (Capsicum annuum L.) by commercial lipases and Pleurotus sapidus extracellular lipase

Zorn, Holger; Breithaupt, Dietmar E.; Takenberg, Meike; Schwack, Wolfgang; Berger, Ralf G.

doi:10.1016/s0141-0229(03)00020-6

Cited by 43 publications

(47 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the usage of enzymes with and without chemical saponification has also been tried to help improve carotenoid recoveries in milk [1,41] and in foods [45,46]. Enzymes (proteases and lipase) with bile salts, co-factors (Mg?)…”

Section: Discussionmentioning

confidence: 99%

Rapid Isocratic HPLC Method and Sample Extraction Procedures for Measuring Carotenoid, Retinoid, and Tocopherol Concentrations in Human Blood and Breast Milk for Intervention Studies

Turner

Burri

2012

Chromatographia

View full text Add to dashboard Cite

Vitamin A deficiency continues to be a major public health concern in the developing world effecting over 200 million people. Interventions including carotenoid-rich fruits and vegetables, containing a-and b-carotene, and b-cryptoxanthin, are being tested for their potential to alleviate vitamin A deficiency in several countries. Thus, there is a need for a faster isocratic reverse-phase high pressure liquid chromatography (HPLC) method than the methods currently available. Efficient extraction procedures for biological samples are also valuable to save time, materials, and cost. We developed an HPLC method and compared several extraction methods for carotenoids, retinoids, and vitamin E in plasma and human breast milk. The method uses an Agilent 1100 system equipped with a Waters Spherisorb ODS2 column (3 9 125 mm 3 lm) and similar guard column (ThermoScientific ODS2 3 9 20 mm 3 lm). Mobile phase of 7:2:1 acetonitrile:dichloromethane: methanol was run at 0.5 mL min -1 , and run times were complete in approximately 8 min. This rapid method uses little solvent and provides excellent results in the analysis of these fat-soluble nutrients. Analyte measurements were repeatable using standards and plasma and were accurate compared to NIST certified and reference serum values. The validated HPLC method was applied to the analysis of plasma samples from a vitamin A intervention study.

show abstract

Section: Discussionmentioning

confidence: 99%

Rapid Isocratic HPLC Method and Sample Extraction Procedures for Measuring Carotenoid, Retinoid, and Tocopherol Concentrations in Human Blood and Breast Milk for Intervention Studies

Turner

Burri

2012

Chromatographia

View full text Add to dashboard Cite

show abstract

“…The P. sapidus strain was grown on agar plates containing malt extract and soybean peptone After 7 days of growth the mycelium was homogenized with water with the use of IKA T25 Basic homogenizer at 8000 rpm and transferred into 200 ml flasks, containing Lindeberg-Holm growth medium for C. unicolor (Lindeberg and Holm, 1952) and standard culture medium suggested by Zorn for P. sapidus (Zorn et al, 2003). The medium was supplemented with Cu 2+ source, without the use of inductors.…”

Section: Methodsmentioning

confidence: 99%

Laccase concentration by foam fractionation of Cerrena unicolor and Pleurotus sapidus culture supernatants

Blatkiewicz

Antecka

Górak

et al. 2017

Chemical and Process Engineering

View full text Add to dashboard Cite

Foam fractionation process for concentration of laccases from two Basidiomycete strains under different process conditions was investigated. Culture supernatants of Cerrena unicolor and Pleurotus sapidus containing active laccase were used with and without surfactant additives. Two surfactants: cationic cetrimonium bromide (CTAB) and non-ionic Polysorbate 80 were applied in the range from 0.2 mM to 1.5 mM. The pH levels ranging from 3 to 10 were examined with particular attention to pH=4, which is close to the pI of the enzymes. Results show that the source of the enzyme is significant in terms of partitioning efficiency in a foam fractionation process. Laccase from Cerrena unicolor showed the best activity partitioning coefficients between foamate and retentate of almost 200 with yields reaching 50% for pH 7.5 and concentration of CTAB c CTAB = 0.5 mM, whereas laccase from Pleurotus sapidus showed partitioning coefficients of up to 8 with 25% yield for pH 4 and c CTAB = 0.5 mM.

show abstract

“…За промислових технологій виробництва курятини виникає ряд факторів, які сприяють зниженню неспецифічного імунітету птиці, а саме значна концентрація поголів'я на обмеженій площі, технологічні стреси, несприятлива екологічна ситуація тощо. Тому для поліпшення збереженості поголів'я, а також стимуляції росту, розвитку та профілактики стресів у годівлі курей м'ясних кросів використовують ряд біологічно активних добавок, які володіють антиоксидантними, провітамінними, антиканцерогенними, гепатопротекторними та імуностимулюючими властивостями (Batiuzhevskyi, 2003;Denys, 1973;Zorn et al, 2003). До таких сполук відноситься β-каротин біотехнологічного синтезу, який одержують культивуванням гриба Bl.…”

Section: продуктивність та неспецифічна резистентність курчат-бройлерівunclassified

Продуктивність Та Неспецифічна Резистентність Курчат-Бройлерів За Дії Β-Каротину

2017

View full text Add to dashboard Cite

Use in feeding broiler chickens of Vitaton and Vitadeps with and without butylhydroxytoluene (BHT) improves feed intake and water, and has no effect on body weight. The feeding of broiler chickens with mix fodder of Vitaton and BHT in doses that meet and exceed the physiological need of β-carotene calculated for retinol and Vitaton with no OSH in a normal dose of β-carotene did not significantly alter the absolute number of lymphocytes blood and the ratio of their subpopulation responsible for cellular (T-lymphocytes) and humoral (B-lymphocytes) immunity. Phagocytic activity of neutrophils and phagocytic index were not significantly changed in the peripheral blood of broiler chickens at feeding Vitaton as a source of β-carotene with/without BHT. Use of Vitaton without BHT in feeding of broiler chickens at a dose of 0.7 g/kg caused a lymphocytopenia. In chickens of this group we noted a decrease in the total number of lymphocytes in the blood due to decrease of cellular and humoral immunity, namely all subpopulations of T-lymphocytes, including T-helper cells -by 58.3 %, T-suppressors -by 41.0, T-helper cells activeat 43.5, B-lymphocytes by 60.1 %, and 0-lymphocytes -by 40.0 %. This is consistent with the reduction in titer of natural antibodies in their serum by 35.3 %. This phagocytic activity of neutrophils and index of phagocytes in blood of broiler chickens unchanged compared with the control. Use of Vitadeps in feeding broiler chickens at a dose of 5.6 g/kg in mix fodder also caused lymphocytopenia. This was reflected in the decrease of absolute number of blood 0-lymphocytes by 43.4 %, Tsuppressors -by 38.7 % and T-helper cells active -by 39.0 %, but their ratio in the blood was like control level. Thus, usage of Vitaton and Vitadeps with/without butylhydroxytoluene in doses that meet the bird demands of β-carotene calculated to vitamin A, ensure the normal functioning of immune organs. Use of Vitaton with BHT as a source of β-carotene for the feeding of broiler chickens at a dose exceeding the normal content of vitamin A by 7 times, does not cause the violations of immunological homeostasis of the poultry. The Vitaton without butylhydroxytoluene and Vitadeps in doses exceeding the normal content of bird β-carotene calculated to vitamin A by 7 times depressing the immunopoesis in broiler chickens. Key words: Vitaton; Vitadeps; β-carotene; broiler chickens; weight; lymphocytes; phagocytic activity Продуктивність та неспецифічна резистентність курчат-бройлерів за дії β-каротинуНаціональний університет біоресурсів і природокористування України, м. Київ 2 Вінницький національний аграрний університет, м. Вінниця, Україна E-mail: shevchenko_laris@ukr.net, dep_rector@vsau.vin.ua, vitam@bigmir.net Використання в годівлі курчат-бройлерів вітатону та вітадепсу з бутилгідрокситолуолом (БГТ) та без нього сприяє підвищенню споживання корму, води і не впливає на масу тіла. Згодовування курчатам-бройлерам з комбікормом вітатону з БГТ в дозах, що відповідають і перевищують фізіологічну потребу в β-каротині в перерахунк...

show abstract

Enzymatic hydrolysis of carotenoid esters of marigold flowers (Tagetes erecta L.) and red paprika (Capsicum annuum L.) by commercial lipases and Pleurotus sapidus extracellular lipase

Cited by 43 publications

References 23 publications

Rapid Isocratic HPLC Method and Sample Extraction Procedures for Measuring Carotenoid, Retinoid, and Tocopherol Concentrations in Human Blood and Breast Milk for Intervention Studies

Rapid Isocratic HPLC Method and Sample Extraction Procedures for Measuring Carotenoid, Retinoid, and Tocopherol Concentrations in Human Blood and Breast Milk for Intervention Studies

Laccase concentration by foam fractionation of Cerrena unicolor and Pleurotus sapidus culture supernatants

Продуктивність Та Неспецифічна Резистентність Курчат-Бройлерів За Дії Β-Каротину

Contact Info

Product

Resources

About