Rapid Isocratic HPLC Method and Sample Extraction Procedures for Measuring Carotenoid, Retinoid, and Tocopherol Concentrations in Human Blood and Breast Milk for Intervention Studies

Turner, Tami; Burri, Betty J.

doi:10.1007/s10337-012-2193-9

Cited by 33 publications

(15 citation statements)

References 41 publications

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“…Two hundred µL of human plasma was used in our experiments. In previously published work, quantities such as 200 µL [28,29], 100 µL [23], 800 µL [30], 1 mL [31], and 300 µL [32] of plasma have been required. Isolation of fat-soluble micronutrients from plasma generally consists of two steps.…”

Section: Discussionmentioning

confidence: 99%

Development of an Advanced HPLC–MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma

Hrvolová

Martı́nez-Huélamo

Colmán-Martínez

et al. 2016

IJMS

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The concentration of carotenoids and fat-soluble vitamins in human plasma may play a significant role in numerous chronic diseases such as age-related macular degeneration and some types of cancer. Although these compounds are of utmost interest for human health, methods for their simultaneous determination are scarce. A new high pressure liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) method for the quantification of selected carotenoids and fat-soluble vitamins in human plasma was developed, validated, and then applied in a pilot dietary intervention study with healthy volunteers. In 50 min, 16 analytes were separated with an excellent resolution and suitable MS signal intensity. The proposed HPLC–MS/MS method led to improvements in the limits of detection (LOD) and quantification (LOQ) for all analyzed compounds compared to the most often used HPLC–DAD methods, in some cases being more than 100-fold lower. LOD values were between 0.001 and 0.422 µg/mL and LOQ values ranged from 0.003 to 1.406 µg/mL, according to the analyte. The accuracy, precision, and stability met with the acceptance criteria of the AOAC (Association of Official Analytical Chemists) International. According to these results, the described HPLC-MS/MS method is adequately sensitive, repeatable and suitable for the large-scale analysis of compounds in biological fluids.

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Section: Discussionmentioning

confidence: 99%

Development of an Advanced HPLC–MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma

Hrvolová

Martı́nez-Huélamo

Colmán-Martínez

et al. 2016

IJMS

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“…Retinol, β-carotene, and α-tocopherol were analyzed as described (33), with some modifications: only 100-μL samples were used for analysis with an Agilent Eclipse Plus C18 column (3.5 μm, 2.1 mm × 100 mm) guarded by a Thermo Scientific BDS-hypersil C18 Javelin guard (3 μm, 3 mm × 20 mm) maintained at 30°C. Twenty-five microliters were injected at a flow rate of 0.6 mL/min and a total run time of 4.5 min.…”

Section: Methodsmentioning

confidence: 99%

Vitamin Concentrations in Human Milk Vary with Time within Feed, Circadian Rhythm, and Single-Dose Supplementation

Hampel

Shahab‐Ferdows

Islam

et al. 2017

The Journal of Nutrition

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Background: Human milk is the subject of many studies, but procedures for representative sample collection have not been established. Our improved methods for milk micronutrient analysis now enable systematic study of factors that affect its concentrations.Objective: We evaluated the effects of sample collection protocols, variations in circadian rhythms, subject variability, and acute maternal micronutrient supplementation on milk vitamin concentrations.Methods: In the BMQ (Breast-Milk-Quality) study, we recruited 18 healthy women (aged 18–26 y) in Dhaka, Bangladesh, at 2–4 mo of lactation for a 3-d supplementation study. On day 1, no supplements were given; on days 2 and 3, participants consumed ∼1 time and 2 times, respectively, the US-Canadian Recommended Dietary Allowances for vitamins at breakfast (0800–0859). Milk was collected during every feeding from the same breast over 24 h. Milk expressed in the first 2 min (aliquot I) was collected separately from the remainder (aliquot II); a third aliquot (aliquot III) was saved by combining aliquots I and II. Thiamin, riboflavin, niacin, and vitamins B-6, B-12, A, and E and fat were measured in each sample.Results: Significant but small differences (14–18%) between aliquots were found for all vitamins except for vitamins B-6 and B-12. Circadian variance was significant except for fat-adjusted vitamins A and E, with a higher contribution to total variance with supplementation. Between-subject variability accounted for most of the total variance. Afternoon and evening samples best reflected daily vitamin concentrations for all study days. Acute supplementation effects were found for thiamin, riboflavin, and vitamins B-6 and A at 2–4 h postdosing, with 0.1–6.17% passing into milk. Supplementation was reflected in fasting, 24-h postdose samples for riboflavin and vitamin B-6. Maximum amounts of dose-responding vitamins in 1 feeding ranged from 4.7% to 21.8% (day 2) and 8.2% to 35.0% (day 3) of Adequate Intake.Conclusions: In the milk of Bangladeshi mothers, differences in vitamin concentrations between aliquots within feedings and by circadian variance were significant but small. Afternoon and evening collection provided the most-representative samples. Supplementation acutely affects some breast-milk micronutrient concentrations. This trial was registered at clinicaltrials.gov as NCT02756026.

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“…Endothelial adhesion molecule concentrations of intercellular adhesion molecule 1 (ICAM 1) and vascular cell adhesion molecule 1 (VCAM-1) were measured by commercially available ELISA kits (Millipore Human Sepsis Panel 1 analysed on the Luminex MagPix ® plate reader). Plasma α-tocopherol levels were determined by reverse-phase high-performance liquid chromatography based on the method of Turner et al [50].…”

Section: Biochemical Analysesmentioning

confidence: 99%

Effect of a 12-Week Almond-Enriched Diet on Biomarkers of Cognitive Performance, Mood, and Cardiometabolic Health in Older Overweight Adults

et al. 2020

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Long term nut consumption is associated with reduced risk of coronary heart disease and better cognitive function. This study examined supplementing habitual diets with almonds or carbohydrate-rich snack foods (providing 15% energy) on biomarkers of cardiovascular and metabolic health, mood and cognitive performance. Participants (overweight/obese, 50-80 years) were randomised to an almond-enriched diet (AED) or isocaloric nut-free diet (NFD) for 12 weeks. Body weight, blood lipids, glucose, insulin, blood pressure (BP), arterial stiffness, cell adhesions molecules, C reactive protein (CRP), mood, and cognitive performance (working memory primary outcome), dietary profiles and energy intake/expenditure were measured at baseline and Week 12 in 128 participants (n = 63 AED, n = 65 NFD). Compared with NFD, AED was associated with altered macro and micronutrient profiles, but no differences in energy intake or expenditure. The AED significantly reduced triglycerides and SBP but there were no other changes in cardiometabolic biomarkers, mood, or cognitive performance. The inclusion of almonds in the diet improves aspects of cardiometabolic health without affecting cognitive performance or mood in overweight/obese adults.Nutrients 2020, 12, 1180 2 of 18 cognitive performance [7]. Nut consumption represents one aspect of nutrition with wide ranging benefits that may counteract these negative influences, including reducing oxidative damage and inflammation, and improving vascular responsiveness [8][9][10]. Improvements in vascular function associated with nut intake are proposed to not only improve cardiovascular health but also contribute to improving cognitive function [11]. Clinical trials have found improvements in vascular health following consumption of walnuts [12], hazelnuts [13], and almonds [14], but this has not been a consistent finding in all studies, possibly due to differences in methods of assessing vascular health, differences in populations evaluated, dose, or length of the period of consumption [9,10].Furthermore, there is now emerging literature to support nut consumption being associated with enhanced cognitive function or reduced cognitive decline [15,16]. Key nutrients found in nuts (and almonds in particular), including monounsaturated fat [17] and vitamin E [18] have been associated with reduced cognitive decline, and it has been proposed that regular nut consumption may prevent or slow the progressing of age-related brain dysfunction [16,19]. Prospective/longitudinal studies have demonstrated a positive association between nut consumption and cognitive performance [20][21][22] with a greater effect in those with the highest nut consumption [21]. In addition, prospective cohort studies reported improved cognitive performance and a reduced incidence of cognitive decline when the Mediterranean dietary pattern (with nuts as a key component) was adhered to [23,24].There have been mixed results from randomized controlled trials assessing the effects of nut intake on cognition. A 12 week weight loss in...

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Rapid Isocratic HPLC Method and Sample Extraction Procedures for Measuring Carotenoid, Retinoid, and Tocopherol Concentrations in Human Blood and Breast Milk for Intervention Studies

Cited by 33 publications

References 41 publications

Development of an Advanced HPLC–MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma

Development of an Advanced HPLC–MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma

Vitamin Concentrations in Human Milk Vary with Time within Feed, Circadian Rhythm, and Single-Dose Supplementation

Effect of a 12-Week Almond-Enriched Diet on Biomarkers of Cognitive Performance, Mood, and Cardiometabolic Health in Older Overweight Adults

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