Carotid glomectomy in rats reduced daily water consumption and increased daily consumption of NaCl solution. Sham operation did not modify water and salt consumption. Intraperitoneal injection of angiotensin-II did not stimulate drinking motivation in the majority of rats subjected to carotid glomectomy. Injection of angiotensin-II to sham-operated and intact animals induced active consumption of both fluids during one hour. These results attest to the involvement of the carotid body in the regulation of consumption of water and sodium ions (the main elements of osmotic blood pressure) and the involvement of angiotensin-sensitive receptors of carotid body cells in the formation of thirst and salt appetite motivation, regulated by the renin-angiotensin system.

The results of studies of the content of residues of veterinary drugs in food indicate that when growing all types of productive animals, a significant number of antibiotics, sulfonamide and nitrofuran preparations of various spectrum and mechanism of action accumulate in meat, milk, eggs, as well as for preventive and therapeutic purposes and honey. We registered that food products of animal origin contain residues of veterinary preparations that do not exceed the MPC. Despite the ban on the use of antibiotics as stimulators of animal productivity, they are more often found in pork and beef, which indicates the use of these antimicrobials in the production of productive animals. Chloramphenicol was most often found in beef and pork, in rabbit meat, goose meat, chicken meat and honey, this antibiotic was present only in sporadic cases, and in milk its presence was not proven during monitoring studies. Nitrofuran preparations were detected mainly in beef, chicken, goose and honey, and in the last reporting year (2016), their presence was not found in these products, except for honey. Studies have shown that food eggs did not contain residues of nitrofuran drugs during the study period. The use of antibacterial drugs and anthelmintics in livestock breeding is confirmed by the fact that about 40% of the samples contain residual amounts of sulfanilamide preparations in the range of 50-70% of the MPC and about 20% of the samples contain residual amounts of anthelmintics. In Ukraine, control of the remnants of veterinary drugs in food products formally is not carried out. Of the group of fluoroquinolones, only enrofloxacin and norfloxacin are monitored, of the B-lactam group, amoxicillin, ampicillin, benzylpencilin from the aminoglycoside group-gentamicin, lincomycin, streptomycin, and dihydrostreptomycin. As for sulfonamide drugs, control is conducted only for 9 seconds with a very large group of these drugs. Particular attention when controlling the content of residues of veterinary drugs in livestock products should be given to methods for identifying these substances and improving existing research methods. We also suggested to extend the indicators mentioned in the "State Monitoring Plan for Residual Amounts of Veterinary Preparations, Pollutants and Toxicants in Products of Animal Origin" for greater control over the animal production and use of antibacterial substances in the farms. Key words: veterinary drugs; antibiotics; chloramphenicol; nitrofurans; hormonal substances; liquid chromatography method; suitability of the method Державний науково-дослідний інститут з лабораторної діагностики та ветеринарно-санітарної експертизи 2 Національний університет біоресурсів і природокористування України, м. Київ E-mail: kot30@meta.ua,shevchenko_laris@ukr.net, vitam@bigmir.netРезультати досліджень вмісту залишків ветеринарних препаратів у харчових продуктах свідчать, що при вирощуванні всіх видів продуктивних тварин широко застосовуються як з профілактичною, так і лікувальною метою значну кількість антибіотиків, сульфан...

Use in feeding broiler chickens of Vitaton and Vitadeps with and without butylhydroxytoluene (BHT) improves feed intake and water, and has no effect on body weight. The feeding of broiler chickens with mix fodder of Vitaton and BHT in doses that meet and exceed the physiological need of β-carotene calculated for retinol and Vitaton with no OSH in a normal dose of β-carotene did not significantly alter the absolute number of lymphocytes blood and the ratio of their subpopulation responsible for cellular (T-lymphocytes) and humoral (B-lymphocytes) immunity. Phagocytic activity of neutrophils and phagocytic index were not significantly changed in the peripheral blood of broiler chickens at feeding Vitaton as a source of β-carotene with/without BHT. Use of Vitaton without BHT in feeding of broiler chickens at a dose of 0.7 g/kg caused a lymphocytopenia. In chickens of this group we noted a decrease in the total number of lymphocytes in the blood due to decrease of cellular and humoral immunity, namely all subpopulations of T-lymphocytes, including T-helper cells -by 58.3 %, T-suppressors -by 41.0, T-helper cells activeat 43.5, B-lymphocytes by 60.1 %, and 0-lymphocytes -by 40.0 %. This is consistent with the reduction in titer of natural antibodies in their serum by 35.3 %. This phagocytic activity of neutrophils and index of phagocytes in blood of broiler chickens unchanged compared with the control. Use of Vitadeps in feeding broiler chickens at a dose of 5.6 g/kg in mix fodder also caused lymphocytopenia. This was reflected in the decrease of absolute number of blood 0-lymphocytes by 43.4 %, Tsuppressors -by 38.7 % and T-helper cells active -by 39.0 %, but their ratio in the blood was like control level. Thus, usage of Vitaton and Vitadeps with/without butylhydroxytoluene in doses that meet the bird demands of β-carotene calculated to vitamin A, ensure the normal functioning of immune organs. Use of Vitaton with BHT as a source of β-carotene for the feeding of broiler chickens at a dose exceeding the normal content of vitamin A by 7 times, does not cause the violations of immunological homeostasis of the poultry. The Vitaton without butylhydroxytoluene and Vitadeps in doses exceeding the normal content of bird β-carotene calculated to vitamin A by 7 times depressing the immunopoesis in broiler chickens. Key words: Vitaton; Vitadeps; β-carotene; broiler chickens; weight; lymphocytes; phagocytic activity Продуктивність та неспецифічна резистентність курчат-бройлерів за дії β-каротинуНаціональний університет біоресурсів і природокористування України, м. Київ 2 Вінницький національний аграрний університет, м. Вінниця, Україна E-mail: shevchenko_laris@ukr.net, dep_rector@vsau.vin.ua, vitam@bigmir.net Використання в годівлі курчат-бройлерів вітатону та вітадепсу з бутилгідрокситолуолом (БГТ) та без нього сприяє підвищенню споживання корму, води і не впливає на масу тіла. Згодовування курчатам-бройлерам з комбікормом вітатону з БГТ в дозах, що відповідають і перевищують фізіологічну потребу в β-каротині в перерахунк...

In contrast to normal environmental conditions (normal temperature and gaseous composition of the atmosphere), regular exposure to hypoxia or cold for 3 weeks after carotid glomectomy does not increase blood glucose and causes no anemia or depression of gaseous and energy metabolism. Diabetogenic effect of streptozotocin after a previous hypoxic or cold exposure is associated with lower hyperglycemia; the time com~e of hematological and gaseous metabolism parameters after glomectomy depends on the effects of changed gaseous environment or temperature.The physiological significance of sinocarotid reflexogenie zones in neurohumoral regulation of the glucoreceptor function of the carotid chemoreceptors [8,10] prompted us to measure the level of glycemia and the main parameters of red blood and gaseous exchange in glomectomyzed diabetic animals under conditions of normoxia, hypoxia, and hypothermia.

Carotenoids that do not have provitamin activity – lycopene and astaxanthin can accumulate in the yolks of chicken eggs and give them colour, as well as affect the metabolism of lipids and fatty acids, which determine the biological value and functional capacity of such products. The aim of this study was to determine the fatty acid composition of egg yolk lipids by adding oily extracts of lycopene (20, 40 and 60 mg/kg feed) or astaxanthin (10, 20 and 30 mg/kg feed) to the diet of laying hens. 45 High Line W36 chickens at the age of 24 weeks were used for the experiment. It was found that the addition of lycopene at doses of 20, 40 and 60 mg/kg and astaxanthin at doses of 10, 20 and 30 mg/kg of feed for 30 days did not significantly affect the ratio of saturated and most monounsaturated fatty acids in egg yolks and Σ SFA and Σ MUFA. Lycopene at a dose of 20 mg/kg of feed reduced the content of cis-11-eicosenoic acid, and astaxanthin at a dose of 10 mg/kg of feed reduced the content of palmitoleic acid by increasing the proportion of cis-10-heptadecenoic acid in the lipids of egg yolks. Addition of lycopene to the feed of laying hens at a dose of 20 mg/kg of feed caused a decrease in the particles of linoleic, which belongs to ω6 PUFA, and cis-eicosenoic acids. Astaxanthin enrichment of the diet of laying hens at a dose of 30 mg/kg reduced the proportion of cis-4,7,10,13,16,19-docosahexaenoic acid, which belongs to ω3 PUFA, in the lipids of the yolks. Σ PUFA decreased in the lipid fraction of yolks only under the influence of lycopene supplements at a dose of 20 mg/kg of feed. Feeding of laying hens with lycopene and astaxanthin supplements did not affect Σ ω3 PUFA and Σ ω6 PUFA. Lycopene at a dose of 20 mg/kg decreased, and astaxanthin at a dose of 30 mg/kg of feed increased ω3/ω6 PUFA in lipids of egg yolks. The results of the research can be used to select oils in combination with carotenoids of natural origin in the diet of chickens during the creation of a model of enrichment of egg yolks by individual representatives of ω3 PUFA and ω6 PUFA.

The uncontrolled antimicrobial agents use leads to the spread of antibiotic-resistant pathogenic strains. The ingress of antibacterial drugs into surface water through sewage from livestock premises, people's houses, hospitals and during medical and agronomic activities exacerbates this problem. Antimicrobials in surface waters have several ways of developing the process: lethal interaction of antibiotics with hydrobionts, elimination of sensitive taxa and alteration of trophic chains; neutralization of antimicrobials (self-destruction of the molecule, hydrolysis, sorption, abiotic and biotic transformation); non-lethal interaction of antibiotics and hydrobionts with the acquisition of antibiotic resistance signs). There are following indicators proposed to predict the antimicrobial agents impact on the aquatic ecosystem: accumulation factor; the amount of substance per unit mass of plant or animal in one cubic meter of water (mg or mcg per 1m3); the maximum amount of a substance that can be contained in an ecosystem without disturbing its basic trophic properties. The basic principles of a dynamic chamber model construction for studying the effect of antibiotics on the ecosystem are based on the following basic statements: 1) the trophic chain is divided into the chambers in which substance is instantaneously mixed in all parts of the chamber in the same way in any direction; 2) the transfer of the substance from one chamber to another occurs according to the laws of first order kinetics, which is described by the system of differential equations. In this case, transition coefficients of a substance between the chambers are constant. It is advisable to use a dynamic chamber model to analyze the migration pathways of substances in a freshwater non-flowing reservoir. For instance, its simplified version consists of the 3 chambers, namely: water – sediments (silt) – biota. It is advisable to use a stationary chamber model for analysing the pathways of antibiotics which enter into the sea with the river flows.

One of the modern antibacterial agents that are an alternative to antibiotics are nanoparticles of noble metals, including silver. To reduce their toxicity, cumulative effect and prolong the effect in animals, there is ongoing work on development and improvement of the methods for their synthesis using various carriers, including those based on polymer/inorganic hybrids. In this study, the quality and safety of edible eggs were determined on Hy-Line laying hens using W36 solutions of nanosilver in carriers based on polymer/inorganic hybrids (AgNPs/SPH) in the concentration of 0.0, 1.0 and 2.0 mg/L of water (0.0, 0.2 and 0.4 mg per hen per day) three times with 10 day interval. We determined that one-, two- and three-time feeding of nanosilver in doses of 0.0, 0.2 and 0.4 mg per hen per day did not affect water consumption, feed, egg productivity, as well as dry matter content, crude protein, fat, ash, and calcium and phosphorus in eggs for 30 days. Contamination of the surface of the shell and yolks of eggs with mesophilic aerobic and facultative anaerobic microorganisms (MAFAnM) did not depend on the dose and duration of consumption of the nanosilver drug by laying hens. The nanosilver drug in doses of 0.0, 0.2 and 0.4 mg per hen per day did not affect the contamination of the egg shell surface with microorganisms of genera Citrobacter, Klebsiella, as well as Escherichia coli, Proteus mirabilis, Salmonella spp., Staphylococcus aureus and S. epidermidis. When administered orally, nanosilver in the dose of 0.2 mg per hen per day did not change the number of symbiotic microorganisms of genera Bifidobacterium and Lactobacillus, while and the dose of 0.4 mg per hen daily slightly reduced the number of microorganisms of genus Lactobacillus in the hens’ manure. The obtained data can be used for further research to determine the effective dose and interval of application of nanosilver preparations to poultry for preventive and therapeutic measures, taking into account the preservation of the microbiome of the digestive system of hens.

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