Enzymatic hydrolysis of cactus pear varieties with high solids loading for bioethanol production

Alencar, Bárbara Ribeiro Alves; Dutra, Emmanuel Damilano; Sampaio, Everardo Valadares de Sá Barretto; Menezes, Rômulo Simões Cézar; Morais, Marcos Antônio de

doi:10.1016/j.biortech.2017.11.042

Cited by 22 publications

(15 citation statements)

References 36 publications

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“…It is important to note that the raw material was previously hydrolyzed with dilute acid and commercial enzymes, which allowed the release of higher concentrations of fermentable sugars while in this work none previous hydrolysis was carried out. Alencar et al [28] also obtained a very similar alcohol value, 26.4 g/L using O. ficus-indica and K. marxianus (with previous acid and enzymatic hydrolysis). The hydrolysis process in the present work was exclusively biological since it was carried out by the yeast itself (with a previous thermal pretreatment, consisting of a sterilization of the medium of 121 °C, 15 min), which demonstrated hydrolytic activity on the raw material.…”

Section: Resultsmentioning

confidence: 52%

Enzymatic hydrolysis of Opuntia ficus-indica cladode by Acinetobacter pittii and alcohol fermentation by Kluyveromyces marxianus: pH, temperature and microorganism effect

López-Domínguez¹,

Ramírez‐Sucre²,

Rodríguez‐Buenfil³

2019

Biotechnology Reports

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Section: Resultsmentioning

confidence: 52%

Enzymatic hydrolysis of Opuntia ficus-indica cladode by Acinetobacter pittii and alcohol fermentation by Kluyveromyces marxianus: pH, temperature and microorganism effect

López-Domínguez¹,

Ramírez‐Sucre²,

Rodríguez‐Buenfil³

2019

Biotechnology Reports

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“…Hydrolysis consists of the degradation of polysaccharides in aqueous media that can be catalyzed by enzymes, acids, or bases to produce glucose [13,36,42,[49][50][51][52][53][54][55][56][57][58][59][60][61][62][63]. The hydrolysis result will depend on the reaction of enzymes and compounds [64][65][66][67].…”

Section: Hydrolysismentioning

confidence: 99%

“…Biomass is a renewable energy source that has received attention; various types of biomass have been used such as coffee grounds [9], tea waste, and palm shells [10,11]. Bio-solvents are solvents that can be produced easily and economically where the production process consists of several stages: hydrolysis, fermentation, and the purification process through distillation [12][13][14][15][16][17][18]. One type of bio-solvent that can be produced from biomass is bioethanol [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Review of Solvents Based on Biomass for Mitigation of Wax Paraffin in Indonesian Oilfield

et al. 2019

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This paper presents a review of the expectations and challenges of using biomass in the prevention and slowing of paraffin wax deposition that takes place during the crude oil production process. The inhibition of the deposition process involves the use of solvents from biomass that are generally available around the crude oil production field. The processes used to scale down the precipitation of wax include mixing crude oil with the manufacturer's solvent composed of toluene and xylene. The goal is to assess solvents sourced from biomass that are capable to slow down the wax deposition process. Wax appearance temperature is an important characteristic to evaluate the possible wax precipitation of a given fluid. Wax precipitation can be reduced by using some chemical additives, often called the pour point depressant. This additive is expected to be produced from local biomass which can compete with solvents currently produced on the market.

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“…Pectinase is used industrially to process fibers for textiles, to clarify wine and fruit juices in pulp and paper processing and as supplement in animal feed [1,[25][26][27][28]. While a wide range of plants produce pectinase [29] commercial production is usually performed by microbial fermentation [15,[30][31][32][33][34]. Till date pectinases have been isolated from various microbial sources such as bacteria [35], yeast [36], fungi [37] and actinomycetes [38] for broad application.…”

Section: Introductionmentioning

confidence: 99%

Genomic, Transcriptomic and Enzymatic Insight into Lignocellulolytic System of a Plant Pathogen Dickeya sp. WS52 to Digest Sweet Pepper and Tomato Stalk

et al. 2019

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Dickeya sp., a plant pathogen, causing soft rot with strong pectin degradation capacity was taken for the comprehensive analysis of its corresponding biomass degradative system, which has not been analyzed yet. Whole genome sequence analysis of the isolated soft-rotten plant pathogen Dickeya sp. WS52, revealed various coding genes which involved in vegetable stalk degradation-related properties. A total of 122 genes were found to be encoded for putative carbohydrate-active enzymes (CAZy) in Dickeya sp. WS52. The number of pectin degradation-related genes, was higher than that of cellulolytic bacteria as well as other Dickeya spp. strains. The CAZy in Dickeya sp.WS52 contains a complete repertoire of enzymes required for hemicellulose degradation, especially pectinases. In addition, WS52 strain possessed plenty of genes encoding potential ligninolytic relevant enzymes, such as multicopper oxidase, catalase/hydroperoxidase, glutathione S-transferase, and quinone oxidoreductase. Transcriptome analysis revealed that parts of genes encoding lignocellulolytic enzymes were significantly upregulated in the presence of minimal salt medium with vegetable stalks. However, most of the genes were related to lignocellulolytic enzymes, especially pectate lyases and were downregulated due to the slow growth and downregulated secretion systems. The assay of lignocellulolytic enzymes including CMCase and pectinase activities were identified to be more active in vegetable stalk relative to MSM + glucose. However, compared with nutrient LB medium, it needed sufficient nutrient to promote growth and to improve the secretion system. Further identification of enzyme activities of Dickeya sp.WS52 by HPLC confirmed that monosaccharides were produced during degradation of tomato stalk. This identified degradative system is valuable for the application in the lignocellulosic bioenergy industry and animal production.

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Enzymatic hydrolysis of cactus pear varieties with high solids loading for bioethanol production

Cited by 22 publications

References 36 publications

Enzymatic hydrolysis of Opuntia ficus-indica cladode by Acinetobacter pittii and alcohol fermentation by Kluyveromyces marxianus: pH, temperature and microorganism effect

Enzymatic hydrolysis of Opuntia ficus-indica cladode by Acinetobacter pittii and alcohol fermentation by Kluyveromyces marxianus: pH, temperature and microorganism effect

Review of Solvents Based on Biomass for Mitigation of Wax Paraffin in Indonesian Oilfield

Genomic, Transcriptomic and Enzymatic Insight into Lignocellulolytic System of a Plant Pathogen Dickeya sp. WS52 to Digest Sweet Pepper and Tomato Stalk

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