Genomic, Transcriptomic and Enzymatic Insight into Lignocellulolytic System of a Plant Pathogen Dickeya sp. WS52 to Digest Sweet Pepper and Tomato Stalk

Yang, Yingjie; Lin, Wei; Singh, Raghvendra Pratap; Xu, Qian; Zhihou, Chen; Yuan, Yuan; Zou, Ping; Li, Yiqiang; Zhang, Chengsheng

doi:10.3390/biom9120753

Cited by 19 publications

(4 citation statements)

References 59 publications

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“…CAZy annotation showed that there are more genes in A. japonicas PJ01 related to carbohydrate activity than Dickeya sp. WS52, which can also produce pectinase, cellulase and xylanase [38]. Xylanase can be used as an auxiliary enzyme to improve the hydrolysis efficiency of cellulase [39].…”

Section: Discussionmentioning

confidence: 99%

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Qian

Gao

Wang

et al. 2021

Foods

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Aspergillus japonicas PJ01 (A. japonicas PJ01) is a strain isolated from the rotten branches. In previ-ous studies, it was shown that it can produce complex enzymes to degrade polysaccharide com-ponents. In this study, we evaluated the safety of its crude enzyme solution. Acute oral toxicity, subchronic toxicity, micronucleus and sperm malformation tests all validated the high biologi-cal safety for the crude enzymes. Secondly, we carried out the citrus segment degradation ex-periment of crude enzyme solution. Compared with the control group, the crude enzyme solu-tion of A. japonicas PJ01 can completely degrade the segments in 50 min, which provides the basis for enzymatic peeling during juice processing. The whole genome sequencing showed that the genome of A. japonicus PJ01 has a GC content of 51.37% with a size of 36204647 bp, and encoded 10070 genes. GO, COG, KEGG and CAZy databases were used in gene annotation analyses. Pathway enrichment showed many genes related to carbohydrate metabolism, rich in genes re-lated to pectinase, xylanase and carboxylcellulase. Therefore, the complex enzyme produced by A. japonicus PJ01 can be used in gizzard juice processing to achieve efficient enzymatic decapsu-lation.

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Section: Discussionmentioning

confidence: 99%

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Qian

Gao

Wang

et al. 2021

Foods

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“…When the germinating spores were separated from the chitosans, the spore germination and infection against F. concentricum were recovered, whereas no water‐soaked lesions and soft rot were observed at the infection sites. Generally, soft rot is due to the secretion of a battery of pectinases which acts by destroying the integrity of the plant cell wall (Yang, Lin et al, 2019). In this study, 0.5 g/L of HMC or WSC may inactivate the pectinase activity, which to some extent damaged the infection capacity of F .…”

Section: Discussionmentioning

confidence: 99%

Inhibition effect of preservatives or disinfectants on F. concentricum from postharvest asparagus ( Asparagus officinalis L.) spear in vitro and in vivo

Zhang

Pan

Yonggang

et al. 2021

Food Processing Preservation

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In this study, the antifungal effects of 12 preservatives or disinfectants against Fusarium concentricum on asparagus spear, as well as their inhibiting characteristics, were investigated. Results showed that dehydrogenation sodium acetate at 0.5 g/L, sodium ethyl p‐hydroxybenzoate at 0.3 g/L, sodium hypochlorite at 0.2 g/L, and cinnamic aldehyde at 0.4 g/L could completely inhibit the sporulation. The concentrations of cinnamaldehyde, sodium hypochlorite, H2O2, and ethyl p‐hydroxybenzoate that completely inhibited spore germination were 0.1, 0.1, 0.2, and 0.3 g/L, respectively. High molecule chitosan (HMC) at 0.25 g/L and water‐soluble chitosan (WSC) at 2 g/L could inhibit spore germination and 90% of mycelium growth. Moreover, asparagus treated with HMC or WSC at 0.5 g/L showed hyphae growth with no soft rot, which indicated that pectinase might be damaged. This results might provide an important basis for further study into the uses of preservatives for control of postharvest diseases of asparagus. Novelty impact statement The screened antifungal agents from food additives showed prominent inhibitory effect on F. concentricum, which were expected to be used for postharvest asparagus preservation in the future.

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“…Digging up the genomic, transcriptomic and enzymatic information of a pectin-degrading, lignocellulolytic and plant pathogenic bacteria Dickeya sp. WS52 revealed 122 genes encoding putative carbohydrate-active enzymes (CAZy) and also a higher number of pectin degrading genes [35]. The study further revealed significant up-regulation of the genes encoding lignocellulolytic enzymes in the bacterium in minimal salt medium with vegetable stalks, and the findings constitute a lignocellulolytic system having immense importance in bioenergy industry and animal production.…”

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confidence: 82%

The Biomolecular Spectrum Drives Microbial Biology and Functions in Agri-Food-Environments

et al. 2020

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Microbial biomolecules have huge commercial and industrial potential. In nature, biological interactions are mostly associated with biochemical and biological diversity, especially with the discovery of associated biomolecules from microbes. Within cellular or subcellular systems, biomolecules signify the actual statuses of the microorganisms. Understanding the biological prospecting of the diverse microbial community and their complexities and communications with the environment forms a vital basis for active, innovative biotechnological breakthroughs. Biochemical diversity rather than the specific chemicals that has the utmost biological importance. The identification and quantification of the comprehensive biochemical diversity of the microbial molecules, which generally consequences in a diversity of biological functions, has significant biotechnological potential. Beneficial microbes and their biomolecules of interest can assist as potential constituents for the wide-range of natural product-based preparations and formulations currently being developed on an industrial scale. The understanding of the production methods and functions of these biomolecules will contribute to valorisation of agriculture, food bioprocessing and biopharma, and prevent human diseases related to the environment.

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Genomic, Transcriptomic and Enzymatic Insight into Lignocellulolytic System of a Plant Pathogen Dickeya sp. WS52 to Digest Sweet Pepper and Tomato Stalk

Cited by 19 publications

References 59 publications

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Inhibition effect of preservatives or disinfectants on F. concentricum from postharvest asparagus ( Asparagus officinalis L.) spear in vitro and in vivo

The Biomolecular Spectrum Drives Microbial Biology and Functions in Agri-Food-Environments

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