Enzymatic Analysis of Positional Fatty Acid Distributions in Triacylglycerols by 1(3)-Selective Transesterification with &lt;i&gt;Candida antarctica&lt;/i&gt; Lipase B: a Collaborative Study

Watanabe, Yomi; Sato, Shinichi; Asada, Mihoko; Arishima, Toshiharu; Iida, Yasuhiro; Imagi, Jun; Saito, Katsuyoshi; Sano, Takashi; Sasaki, Akiko; Sasaki, Ryo; Sato, Chiemi; Shibuya, Tadahisa; Tsukahara, Yuki; Nagai, Toshiharu; Fukazawa, Toru; Hori, Ryuji; Homma, Rika; Miyazaki, Yosuke; Yamashita, Atsushi; Yoshinaga, Kazuaki; Watanabe, Shimpei

doi:10.5650/jos.ess15182

Cited by 14 publications

(11 citation statements)

References 10 publications

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“…The linoleic acid yield of trilinolein hydrolysis was 56.8% in this study. Candida antarctica lipase B is reported to cleave triglycerides selectively in position sn-1 and sn-3 (Rogalska et al, 1993;Villeneuve et al, 1995;Watanabe et al, 2015). For long chain fatty acids Candida antarctica lipase B has a low sn-1 selectivity and for short chain fatty acids a low sn-3 selectivity compared to a high typoselectivity for short chain fatty acids (Villeneuve et al, 1995).…”

Section: Enzymatic Hydrolysis Of Triglyceridesmentioning

confidence: 99%

Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194

Gorte

Hollenbach

Papachristou

et al. 2020

Front. Bioeng. Biotechnol.

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Evaluation of SCO Downstream Processing At disruption efficiencies of >90%, lipids can be extracted at high yields, whereas at lower cell disruption efficiencies, considerable amounts of lipids will not be accessible for extraction regardless of the solvents used. Furthermore, it was shown that hexaneethanol which is commonly used for extraction of algal lipids is also highly efficient for yeasts.

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Section: Enzymatic Hydrolysis Of Triglyceridesmentioning

confidence: 99%

Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194

Gorte

Hollenbach

Papachristou

et al. 2020

Front. Bioeng. Biotechnol.

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“…The method has previously been employed in a collaborative study by 12 labs for determining the positional distribution of FAs in liquid and solid vegetable oils soybean and palm oils and PUFA-containing fish oil sardine oil 9 , using the CALB provided by Novozymes Novozym 435 . This paper reports a collaborative study for the analysis of the positional distribution of FAs in milk fat containing SCFAs using the same method, but with a different CALB, Chirazyme L2-C4 supplied by Roche Diagnostics K.K.…”

Section: Introductionmentioning

confidence: 99%

The Collaborative Study on the Enzymatic Analysis of Positional Distribution of Short- and Medium-chain Fatty Acids in Milk Fat Using Immobilized <i>Candida antarctica</i> Lipase B

et al. 2016

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The positional distributions of fatty acids (FAs) in milk fat containing short-and medium-chain FAs were analyzed by sn-1(3)-selective transesterification of triacylglycerols (TAGs) with ethanol using immobilized Candida antarctica lipase B (CALB), in a collaborative study conducted by 10 laboratories. The mean C4:0, C6:0, and C8:0 FA contents, when analyzed as propyl esters (PEs) using gas chromatography (GC) with a DB-23 capillary column, were found to be 3.0, 2.0, and, 1.3 area%, respectively. Their reproducibility standard deviations were 0.33, 0.18, and 0.19, respectively. The mean C4:0, C6:0, and C8:0 contents at the sn-2 position were 0.3, 0.4, and 1.0 area%, respectively. Their reproducibility standard deviations were 0.17, 0.11, and 0.19, respectively. The reproducibility standard deviations of C4:0, C6:0, and C8:0 FAs at the sn-2 position were either the same as or smaller than those for milk fat, although the FA contents at the sn-2 position were smaller than those in the milk fat. Therefore, it was concluded that the CALB method for estimating the regiospecific distribution is applicable to TAGs containing short-and medium-chain FAs. When estimating the short-chain (SC) FA contents in fats and oils by GC, it is better to analyze SCFAs as PEs or butyl esters, and not as methyl esters, in order to prevent loss of SCFAs during the experimental procedure because of their volatility and water solubility. This study also revealed that the stationary phase of the GC capillary column affected the flame ionization detector (FID) response of SCFAs. The theoretical FID correction factor (MW FA / active carbon number / atomic weight of carbon) fitted well with the actual FID responses of C4:0-C12:0 FAs when they were analyzed as PEs using a DB-23 column; however, this was not the case when the GC analysis was performed using wax-type columns.

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“…The structured triglycerols formed a positional analysis and showed a proliferation for the primary positions paralleled to the secondary positions in preference of the lipase action [ 392 , 393 ]. In the Sn-2 position the targeted structured triglycerols with palmitoyl moieties and in the Sn-1, 3 positions of medium chain acyl moieties should be beneficial for infant nutrient and clinical in food formulation as well as parental sustenance uses [ 394 , 395 ]. Lipase isolated from R. miehei was used as the biocatalyst for the acidolysis are commercially immobilized Sn-1, 3-specific lipase, Lipozyme RM IM.…”

Section: Industrial Applications Of Lipasesmentioning

confidence: 99%

Microbial lipases and their industrial applications: a comprehensive review

et al. 2020

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Lipases are very versatile enzymes, and produced the attention of the several industrial processes. Lipase can be achieved from several sources, animal, vegetable, and microbiological. The uses of microbial lipase market is estimated to be USD 425.0 Million in 2018 and it is projected to reach USD 590.2 Million by 2023, growing at a CAGR of 6.8% from 2018. Microbial lipases (EC 3.1.1.3) catalyze the hydrolysis of long chain triglycerides. The microbial origins of lipase enzymes are logically dynamic and proficient also have an extensive range of industrial uses with the manufacturing of altered molecules. The unique lipase (triacylglycerol acyl hydrolase) enzymes catalyzed the hydrolysis, esterification and alcoholysis reactions. Immobilization has made the use of microbial lipases accomplish its best performance and hence suitable for several reactions and need to enhance aroma to the immobilization processes. Immobilized enzymes depend on the immobilization technique and the carrier type. The choice of the carrier concerns usually the biocompatibility, chemical and thermal stability, and insolubility under reaction conditions, capability of easy rejuvenation and reusability, as well as cost proficiency. Bacillus spp., Achromobacter spp., Alcaligenes spp., Arthrobacter spp., Pseudomonos spp., of bacteria and Penicillium spp., Fusarium spp., Aspergillus spp., of fungi are screened large scale for lipase production. Lipases as multipurpose biological catalyst has given a favorable vision in meeting the needs for several industries such as biodiesel, foods and drinks, leather, textile, detergents, pharmaceuticals and medicals. This review represents a discussion on microbial sources of lipases, immobilization methods increased productivity at market profitability and reduce logistical liability on the environment and user.

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Enzymatic Analysis of Positional Fatty Acid Distributions in Triacylglycerols by 1(3)-Selective Transesterification with <i>Candida antarctica</i> Lipase B: a Collaborative Study

Cited by 14 publications

References 10 publications

Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194

Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194

The Collaborative Study on the Enzymatic Analysis of Positional Distribution of Short- and Medium-chain Fatty Acids in Milk Fat Using Immobilized <i>Candida antarctica</i> Lipase B

Microbial lipases and their industrial applications: a comprehensive review

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