Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194

Gorte, Olga; Hollenbach, Rebecca; Papachristou, Ioannis; Steinweg, Christian; Silve, Aude; Frey, Wolfgang; Syldatk, Christoph; Ochsenreither, Katrin

doi:10.3389/fbioe.2020.00355

Cited by 26 publications

(20 citation statements)

References 78 publications

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“…Indirect quantification of produced lipids was conducted by direct transesterification of yeast cell mass to fatty acid methyl esters (FAMEs) as described in Schulze et al [ 15 ] and further optimized in Gorte et al [ 44 ]. 15 mL of fresh cell mass was collected daily from the cultivation broth and washed with 0.9% w/v NaCl at 4800× g for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Gorte

Kugel

Ochsenreither

2020

Biotechnol Biofuels

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Background Biotechnologically produced microbial lipids are of interest as potential alternatives for crude and plant oils. Their lipid profile is similar to plant oils and can therefore be a substitute for the production of biofuels, additives for food and cosmetics industry as well as building blocks for oleochemicals. Commercial microbial lipids production, however, is still not profitable and research on process optimization and cost reduction is required. This study reports on the process optimization using glucose or xylose with the unconventional oleaginous yeast Saitozyma podzolica DSM 27192 aiming to reduce the applied carbon source amount without sacrificing lipid productivity. Results By optimizing the process parameters temperature and pH, lipid productivity was enhanced by 40%. Thereupon, by establishing a two-phase strategy with an initial batch phase and a subsequent fed-batch phase for lipid production in which a constant sugar concentration of about 10 g/L was maintained, resulted in saving of ~ 41% of total glucose and ~ 26% of total xylose. By performing the automated continuous sugar feed the total sugar uptake was improved to ~ 91% for glucose and ~ 92% for xylose and thus, prevented waste of unused carbon source in the cultivation medium. In addition, reduced glucose cultivation resulted in to 28% higher cell growth and 19% increase of lipid titer. By using xylose, the by-product xylonic acid was identified for the first time as by-product of S. podzolica. Conclusions These findings provide a broad view of different cultivation process strategies with subsequent comparison and evaluation for lipid production with S. podzolica. Additionally, new biotechnological characteristics of this yeast were highlighted regarding the ability to produce valuable organic acids from sustainable and renewable sugars.

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Section: Methodsmentioning

confidence: 99%

Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Gorte

Kugel

Ochsenreither

2020

Biotechnol Biofuels

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“…The yeasts growth was monitored by daily sampling and analysis of OD 600nm , CDW, glucose and ammonium consumption. SCO production was indirectly analyzed by gas chromatography via acidic direct transesterification as described in Gorte et al (2020). After 6 days the biomass was harvested and directly further processed.…”

Section: Yeast Cultivationmentioning

confidence: 99%

“…Note that Bligh and Dyer as well as Folch protocols were also tested as described in our previous work (Gorte et al, 2020) but gave lower estimations of the total lipid content.…”

Section: Total Lipidmentioning

confidence: 99%

“…In this context, we have recently evaluated industrially relevant cell disruption methods on laboratory scale using frozen and freeze-dried biomass of the unconventional yeast Saitozyma podzolica DSM 27192 (Gorte et al, 2020). With regard to energy consumption, the direct transesterification of freeze-dried biomass to fatty acid methyl esters (FAMEs) for biodiesel production turned out to be the most energy consuming technique with 5588 kWh/kg FAME ≈ 20116.8 MJ/ kg FAME.…”

Section: Introductionmentioning

confidence: 99%

“…This method can therefore only be considered as an analytical technique. Other tested lipid recovery methods were high pressure homogenization (95 kWh/kg lipid = 342 MJ/kg lipid) and bead milling (96 kWh/kg lipid = 345.6 MJ/kg lipid) of frozen biomass prior to mixed solvent extraction (Gorte et al, 2020). Although latter pre-treatment methods consumed several orders of magnitudes less energy than the first one, they remain too energy consuming.…”

Section: Introductionmentioning

confidence: 99%

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Pulsed Electric Field Treatment Promotes Lipid Extraction on Fresh Oleaginous Yeast Saitozyma podzolica DSM 27192

Gorte

Nazarova

Papachristou

et al. 2020

Front. Bioeng. Biotechnol.

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Pulsed electric field treatment performed on fresh yeast biomass induced permeabilisation of cells and greatly improved the yields of subsequent lipid extraction using ethanol and hexane. of oil raises the competition between food and raw materials production, since agricultural land is limited (Lee and Lavoie, 2013). Into the bargain, an expansion of oil seed cultures would lead to forest land destruction (Escobar et al., 2009). Microbial oils, known as single cell oils (SCO), produced by yeast, microalgae, fungi, and bacteria may overcome all these challenges and act as potential feedstock for crude and plant oil for various applications such as fuels, additives for food and cosmetics, and building blocks for oleochemicals (Ochsenreither et al., 2016; Probst et al., 2016; Vasconcelos et al., 2019). One of the most promising oleaginous microorganisms are yeasts, since they are able to accumulate more than 70% of their cell dry weight (CDW) of lipids (Ratledge, 1991; Probst et al., 2016). The production of SCO by yeasts is independent of season, climate, and location and requires limited amounts of area for cultivation. Additionally, high growth rate and oil productivity are achieved due to short duplication time and the possibility to up-scale cultivation processes, which renders an industrial use realistic (Li et al., 2008; Ageitos et al., 2011). Furthermore, unconventional carbon sources, e.g., lignocellulosic material and waste from food and other industries can be metabolized, enabling waste recycling and guaranteeing a sustainable process

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Oleaginous yeasts: Time to rethink the definition?

López

Vandeputte

Bogaert

2022

Yeast

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Oleaginous yeasts are typically defined as those able to accumulate more than 20% of their cell dry weight as lipids or triacylglycerides. Research on these yeasts has increased lately fuelled by an interest to use biotechnology to produce lipids and oleochemicals that can substitute those coming from fossil fuels or offer sustainable alternatives to traditional extractions (e.g., palm oil). Some oleaginous yeasts are attracting attention both in research and industry, with Yarrowia lipolytica one of the best-known and studied ones. Oleaginous yeasts can be found across several clades and different metabolic adaptations have been found, affecting not only fatty acid and neutral lipid synthesis, but also lipid particle stability and degradation. Recently, many novel oleaginous yeasts are being discovered, including oleaginous strains of the traditionally considered nonoleaginous Saccharomyces cerevisiae. In the face of this boom, a closer analysis of the definition of "oleaginous yeast" reveals that this term has instrumental value for biotechnology, while it does not give information about distinct types of yeasts.Having this perspective in mind, we propose to expand the term "oleaginous yeast"

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Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194

Cited by 26 publications

References 78 publications

Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Pulsed Electric Field Treatment Promotes Lipid Extraction on Fresh Oleaginous Yeast Saitozyma podzolica DSM 27192

Oleaginous yeasts: Time to rethink the definition?

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