Enzymatic activity of alkaline phosphatase adsorbed on dimyristoylphosphatidic acid Langmuir–Blodgett films

Caseli, Luciano; Zaniquelli, Maria Elisabete Darbello; Furriel, Rosa dos Prazeres Melo; Leone, Francisco de Assis

doi:10.1016/s0927-7765(01)00302-2

Cited by 54 publications

(68 citation statements)

References 31 publications

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“…In this sense, to rebuild the surface structure with a high mechanical stability, the conformational and orientation changes must not be highly restricted. In our case, the presence of a phospholipid (hydrophobic anchor) covalently bound to the polypeptidic moiety of the protein gives the GPI-anchored alkaline phosphatase extremely peculiar surface properties, with the GPI-anchor driving the processes of adsorption, orientation, 10,25,[46][47][48] and packing 9 and, as stated by the present work, also influencing the surface elasticity properties. To the best of our knowledge, this is the first report concerning the elasticity properties of a GPI-anchored protein.…”

Section: Elasticity Measurementsmentioning

confidence: 49%

“…Figure 3A brings results for DSAP concentrations below and above CAC of the system (3.6x10 -6 mol L -1 of C 12 E 9 for 180 μg L -1 of the enzyme). 25 It is observed that for an enzyme concentration of 5 μg L -1 , (C 12 E 9 10 -7 mol L -1 ), the system takes 15 minutes to become stable. Above the CAC, a constant surface tension of about 53 mN m -1 is reached in less than 5 min.…”

Section: Resultsmentioning

confidence: 99%

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Adsorption kinetics and dilatational rheological studies for the soluble and anchored forms of alkaline phosphatase at the air/water interface

Caseli¹,

Masui²,

Furriel³

et al. 2005

J. Braz. Chem. Soc.

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Este trabalho apresenta aspectos de equilíbrio e dinâmicos da adsorção na interface ar/líquido de duas formas de fosfatase alcalina de placa óssea de ratos: DSAP, solubilizada com tensoativo não-iônico (C 12 E 9 ), contendo uma âncora de glicosilfosfatidilinositol (GPI), e PLSAP, com a porção hidrofóbica da âncora clivada por fosfolipase-C. A tensão superficial dinâmica, γ dyn , e o módulo de elasticidade superficial dilatacional, ε, foram determinados para soluções de PLSAP, DSAP e C 12 E 9 pelo método de oscilação harmônica e análise do formato da gota eixo-simétrica. Cinéticas de adsorção revelaram que DSAP adsorve trinta vezes mais rapidamente que PLSAP, apresentando um mínimo, e, para PLSAP, a tensão superficial cai continuamente. Para o sistema DSAP/C 12 E 9 , ε atinge um máximo na concentração crítica de agregação (CAC), mas para PLSAP, ε diminui continuamente com a concentração. Soluções de C 12 E 9 apresentam ε mais elevados, decrescentes com a concentração. Um modelo, baseado na influência da âncora GPI, é proposto para explicar os resultados obtidos.This work presents equilibrium and dynamic aspects for the adsorption at the air/liquid interface of two rat osseous plate alkaline phosphatase forms: DSAP, solubilized by a surfactant, C 12 E 9 , and containing a glycosylphosphatidylinositol (GPI) anchor; and PLSAP, resulting from phospholipase-C cleavage of the hydrophobic portion of the GPI anchor. Dynamic surface tension, γ dyn , and surface elasticity modulus, ε, were determined for PLSAP, DSAP and pure C 12 E 9 solutions using harmonic oscillation and axisymmetric drop shape analysis Adsorption kinetics studies revealed that DSAP adsorbs thirty times faster than PLSAP, presenting a minimum in the curve. For DSAP/ C 12 E 9 mixed system, ε increases with concentration and a maximum appears at the critical aggregation concentration (CAC). For PLSAP, a continuous decreasing with concentration for γ dyn and ε was observed. For pure C 12 E 9 solution , the elasticity modulus increases with concentration and ε values are higher when compared to the mixed system. A model based on the influence of the GPI anchor is proposed.Keywords: dynamic surface tension, adsorption kinetics, dilatational surface elasticity, alkaline phosphatase, axisymmetric drop shape analysis IntroductionSurface elasticity is associated with the ability of a system to establish a new surface tension value due to a timely area variation. Considering that it is a nonequilibrium phenomenon, changes in surface tension occur either as a consequence of a sudden compression/ expansion of the interface, or due to a local concentration variation. Since elasticity values are associated with diffusion processes, adsorption/desorption, as well as the rearrangement of molecules at the interface, they depend on the time scale (or frequency) in which the perturbation is applied. In addition, these processes are influenced by molecular weight, shape and molecular interactions of the species present at the interface.Surface elasticity is directly relate...

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Section: Elasticity Measurementsmentioning

confidence: 49%

Section: Resultsmentioning

confidence: 99%

Adsorption kinetics and dilatational rheological studies for the soluble and anchored forms of alkaline phosphatase at the air/water interface

Caseli¹,

Masui²,

Furriel³

et al. 2005

J. Braz. Chem. Soc.

Self Cite

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“…DMPA is one of the most used phospholipids in this context owing to its suitability to deposit multilayers [131,132]. Examples include studies of a mucin protein in LB films of DMPA, where chitosan was able to gradually remove the protein, according to FTIR measurements [116] and interaction studies between DMPA and the protein-polysaccharide complex extracted from the mushroom Agaricus blazei Murill [133].…”

Section: Films Deposited On Solid Substratesmentioning

confidence: 99%

Vibrational spectroscopy for probing molecular-level interactions in organic films mimicking biointerfaces

Volpati

Aoki

Aléssio

et al. 2014

Advances in Colloid and Interface Science

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“…QCM has been used with supported lipid monolayers (Liebau et al, 2001), bilayers (Reimhult et al, 2002a), captured vesicle layers (Reimhult et al, 2003) and LB and Langmuir-Schäfer films (Caseli et al, 2002). Measurements of the viscoelasticity of membranes related to the motional resistance of the sensor, have been used to determine a variety of physical parameters including: adsorption kinetics (Snellings et al, 2001;Eing et al, 2002;Glasmastar et al, 2002;Kastl et al, 2002), lipid-phase transitions (Seantier et al, 2004(Seantier et al, , 2005, lipid leaflet distribution (Richter et al, 2005b), membrane morphology (Ha and Kim, 2001) and a wide variety of other parameters central to supported membrane formation from solution phase (Stalgren et al, 2001(Stalgren et al, , 2002Gotoh and Tagawa, 2002;Reimhult et al, 2002b;Graneli et al, 2003Graneli et al, , 2004Luthgens et al, 2003;Munro and Frank, 2003;Richter et al, 2003;Faiss et al, 2004;Michel et al, 2004;Olofsson et al, 2004;Weng et al, 2004;Reviakine et al, 2005;Richter and Brisson, 2005;Richter et al, 2005a).…”

Section: Lipids and Membranesmentioning

confidence: 99%

A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions

Cooper¹,

Singleton²

2007

J of Molecular Recognition

333

203

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The widespread exploitation of biosensors in the analysis of molecular recognition has its origins in the mid-1990s following the release of commercial systems based on surface plasmon resonance (SPR). More recently, platforms based on piezoelectric acoustic sensors (principally 'bulk acoustic wave' (BAW), 'thickness shear mode' (TSM) sensors or 'quartz crystal microbalances' (QCM)), have been released that are driving the publication of a large number of papers analysing binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This article highlights salient theoretical and practical aspects of the technologies that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and lipidic and polymeric interfaces. Key differentiators between optical and acoustic sensing modalities are also reviewed.

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Enzymatic activity of alkaline phosphatase adsorbed on dimyristoylphosphatidic acid Langmuir–Blodgett films

Cited by 54 publications

References 31 publications

Adsorption kinetics and dilatational rheological studies for the soluble and anchored forms of alkaline phosphatase at the air/water interface

Adsorption kinetics and dilatational rheological studies for the soluble and anchored forms of alkaline phosphatase at the air/water interface

Vibrational spectroscopy for probing molecular-level interactions in organic films mimicking biointerfaces

A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions

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