Vibrational spectroscopy for probing molecular-level interactions in organic films mimicking biointerfaces

Volpati, Diogo; Aoki, Pedro H.B.; Aléssio, Priscila; Pavinatto, Felippe J.; Miranda, Paulo B.; Constantino, Carlos J. L.; Oliveira, Osvaldo N.

doi:10.1016/j.cis.2014.01.014

Cited by 38 publications

(28 citation statements)

References 186 publications

(208 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In order to address these mechanisms, one has to resort to suitable methods not only to mimic the cell membrane but also to characterize photochemical reactions at the molecular level. Currently available methods do not afford interrogation of these effects on whole cells, which is the reason why cell membrane models such as Langmuir monolayers have been used extensively [23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Evidence of photoinduced lipid hydroperoxidation in Langmuir monolayers containing Eosin Y

Pereira

Camacho

Malfatti-Gasperini

et al. 2018

Colloids and Surfaces B: Biointerfaces

Self Cite

View full text Add to dashboard Cite

Photodynamic therapy (PDT) efficiency depends on many factors including the incorporation of the photosensitizer (PS) in cell membranes and possible lipid hydroperoxidation. In this study, we show that hydroperoxidation may be photoinduced when eosin Y is incorporated into Langmuir monolayers that serve as cell membrane models. This occurs for Langmuir monolayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), which have unsaturation in their hydrophobic chains. In contrast, light irradiation had no effect on monolayers of saturated 1,2-dipalmitoyl-sn-glycero-3phosphocholine (DPPC). Evidence of hydroperoxidation was obtained from the area increase in eosin-containing DOPC and POPC monolayers upon irradiation, which was accompanied by a decrease in monolayer thickness according to grazing incidence X-ray off-specular scattering (GIXOS) data. Furthermore, the changes in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) induced by irradiation were consistent with hydroperoxide migration toward the lipid hydrophilic heads.. In summary, this combination of experimental methods allowed us to determine the effects of eosin Y interaction with cell membrane models under irradiation, which may be associated with the underlying mechanisms of eosin Y as photosensitizer in PDT.

show abstract

Section: Introductionmentioning

confidence: 99%

Evidence of photoinduced lipid hydroperoxidation in Langmuir monolayers containing Eosin Y

Pereira

Camacho

Malfatti-Gasperini

et al. 2018

Colloids and Surfaces B: Biointerfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…This unique property has made second‐order NLO techniques unavoidable tools to probe molecular films at interfaces, for unravelling new fundamental knowledge of their physicochemical properties and processes . More specifically, second‐order NLO techniques have set one′s heart on biological interfaces for multiple reasons, first of all the possibility to univocally probe those nano‐bio‐interfaces while surrounded by a macroscopic liquid environment, with the unique advantage that the latter does not contribute to the signal . For example, lipid mono‐ or bilayers mimicking cell membranes are still extensively investigated in interaction with peptides or membrane proteins .…”

Section: Methodsmentioning

confidence: 99%

“…[7,8] More specifically,s econd-order NLO techniques have set one'sh eart on biological interfaces for multiple reasons, first of all the possibility to univocally probe those nanobio-interfaces while surrounded by am acroscopic liquid environment,w ith the unique advantage that the latter does not contributet ot he signal. [9,10,11] For example, lipid mono-or bilayers mimicking cell membranes are still extensively investigated in interaction with peptides or membrane proteins. [12][13][14][15][16][17][18][19][20][21][22][23][24][25] In addition to fundamental research purposes, second-order NLO spectroscopies have provided successful proofs in detecting biological recognition in nanoscale devices.…”

mentioning

confidence: 99%

Unique Vibrational Features as a Direct Probe of Specific Antigen–Antibody Recognition at the Surface of a Solid‐Supported Hybrid Lipid Bilayer

Lis

Cecchet

2016

ChemPhysChem

View full text Add to dashboard Cite

Here, we demonstrate how sum frequency generation (SFG), a vibrational spectroscopy based on a nonlinear three-photon mixing process, may provide a direct and unique fingerprint of bio-recognition; This latter can be detected with an intrinsically discriminating unspecific adsorption, thanks to the high sensitivity of the second-order nonlinear optical (NLO) response to preferential molecular orientation and symmetry properties. As a proof of concept, we have detected the biological event at the solid/liquid interface of a model bio-active antigen platform, based on a solid-supported hybrid lipid bilayer (ss-HLB) of a 2,4-dinitrophenyl (DNP) lipid, towards a monoclonal mouse anti-DNP complementary antibody.

show abstract

“…PM-IRRAS, which is insensitive to IR absorption of water vapor, was introduced to avoid such problems. In PM-IRRAS, the incident beam is alternated between s-and p-polarization at a frequency of tens of kHz [63] and differential reflectivity is calculated. Besides these two IR spectroscopic techniques, there are others such as surface-enhanced infrared-absorption spectroscopy (SEIRA) and attenuated total reflection ATR-FTIR spectroscopy.…”

Section: Infrared Spectroscopymentioning

confidence: 99%

Monolayers of Carbohydrate-Containing Lipids at the Water- Air Interface

Nepal¹,

Stine²

2019

Cell Culture

View full text Add to dashboard Cite

Glycolipids are important members of the glycoconjugate family that are distributed on cell surfaces and are important in aspects of cellular behavior including signal transduction, protein trafficking, cell surface recognition and cell adhesion. Errors in the synthesis or mutations of these glycoconjugates are often linked with various human pathological conditions. The complex nature of their molecular structures coupled with the complexity of cellular structure make their study a challenging process, which can be simplified by fabrication of model membrane systems. Liposomes and monolayers of lipids at the airwater interface are two of the most frequently used model membrane systems. Techniques for fabrication of monolayer models and methods used for their studies are discussed with a focus on glycolipids.

show abstract

Vibrational spectroscopy for probing molecular-level interactions in organic films mimicking biointerfaces

Cited by 38 publications

References 186 publications

Evidence of photoinduced lipid hydroperoxidation in Langmuir monolayers containing Eosin Y

Evidence of photoinduced lipid hydroperoxidation in Langmuir monolayers containing Eosin Y

Unique Vibrational Features as a Direct Probe of Specific Antigen–Antibody Recognition at the Surface of a Solid‐Supported Hybrid Lipid Bilayer

Monolayers of Carbohydrate-Containing Lipids at the Water- Air Interface

Contact Info

Product

Resources

About