A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions

Abstract

The widespread exploitation of biosensors in the analysis of molecular recognition has its origins in the mid-1990s following the release of commercial systems based on surface plasmon resonance (SPR). More recently, platforms based on piezoelectric acoustic sensors (principally 'bulk acoustic wave' (BAW), 'thickness shear mode' (TSM) sensors or 'quartz crystal microbalances' (QCM)), have been released that are driving the publication of a large number of papers analysing binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This article highlights salient theoretical and practical aspects of the technologies that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and lipidic and polymeric interfaces. Key differentiators between optical and acoustic sensing modalities are also reviewed.

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

“…[58][59][60][61][62] Efficient immobilization protocols yielding high surface coverage, well-established alignment and proper orientation of single-strand DNA (ssDNA) on surfaces as a probe molecule are primary issues in the construction of high-performance DNA-based sensors. The immobilization of probe ssDNA molecules onto a gold surface is generally accomplished by using sulfanylanchored DNA (DNA-SH; It was well known that 'mercapto-' and '-thiol' have been used in the scientific literature as prefix and suffix, respectively.…”

Real-time monitoring of electrochemically active biofilm developing behavior on bioanode by using EQCM and ATR/FTIR

“…Especially, Sauerbrey equation will underestimate biomass because the biofilm is elastic and dissipation energy was not considered (Voinova et al 1999). Just like Cooper et al suggested that QCM is primarily sensitive to interactions local to the quartz surface and not to multi-cellular layers (Cooper and Singleton 2007). But QCM technique still is attractive for the study of changes in surface adherent cells (1993;Kleijin et al 2010;Redepenning et al 1993).…”

“…Quartz Crystal Microbalance (QCM) can provide the attached cells as sensed biological element via a piezoelectric mechanism for signal transduction. QCM as a technique to examine interfacial phenomena has been focused on studies of living cells for the process of cellular adhesion for traditional biofilms (Cooper and Singleton 2007;Speight and Cooper) such as osteoblasts (Redepenning et al 1993), human platelets (Muratsugu et al 1997), other mammalian cells (Wegener et al 1998), etc. to reveal normal cell phenotype.…”

Determinação dos parâmetros cinéticos e termodinâmicos da adsorção de L-cisteína em ouro por meio da técnica de microbalança a cristal de quartzo

“…[22][23][24] Esse material pode ser posto a oscilar através da aplicação de uma diferença de potencial alternada, sendo que sua frequência de oscilação é monitorada em função do tempo. Por meio da relação de Sauerbrey 24,25 (Equação 1) é possível relacionar a variação de frequência de oscilação do cristal com a massa aderida em sua superfície.…”

“…Nesses casos, apenas os valores de frequência não são adequados para estimar a massa aderida sobre o substrato e se faz necessário conhecer os valores de dissipação energética (fator D), fornecendo informações a respeito da viscoelasticidade da camada formada. 24 De forma geral, quanto maior for a viscoelasticidade da camada formada, maior será o amortecimento e, portanto, o valor de D. Esse parâmetro é definido como 24 (Equação 3):…”