Enzymatic activities and pathogenesis-related genes expression in sunflower inbred lines affected by<i>Sclerotinia sclerotiorum</i>culture filtrate

Monazzah, Maryam; Enferadi, Sattar Tahmasebi; Rabiei, Zohreh

doi:10.1111/jam.13766

Cited by 6 publications

(3 citation statements)

References 83 publications

(158 reference statements)

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“…Most pathogenesis-related proteins have low molecular weight ranging from 8 kDa to 50 kDa [22]. In our previous study, we observed that the transcript abundance of two pathogenesis-related genes, PDF 1.2 and PR5-1, were higher in the partially resistant line compared to those in the susceptible line [23,24]. However, the identities of these new proteins need to be more clarify in future investigations.…”

Section: Protein Patterns Of Total Proteins In Susceptible and Partiamentioning

confidence: 99%

“…The Phloroglucinol-HCl staining also showed that more lignin deposition in the cell walls of xylem of partially resistant line after pathogen filtrate treatment. In our previous studies [23,24], we found that the activity of phenylalanine ammonia lyase enzyme (PAL) as well as shikimate dehydrogenase enzyme were much more pronounced in partially resistant line than susceptible line. In addition, some proteins such as peroxidase and laccase were only up-regulated in partially resistant line that might point out the critical role of the phenylpropanoid pathway in sunflower defense mechanism against S. sclerotiorum infection.…”

Section: Changes In Xylem Vessels Of Sunflower Lines After Culture Fimentioning

confidence: 99%

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Changes in Anatomical Features and Protein Pattern of Sunflower Partially Resistant and Susceptible Lines During Infection By Virulence Factors of Sclerotinia Sclerotiorum

Monazzah¹,

Enferadi

Rabiei³

et al. 2019

Phyton

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Helianthus annuus L. as an oil seed crop is widely grown throughout the world. One of the most destructive diseases of sunflower is stem rot caused by Sclerotinia sclerotiorum. Oxalic acid is the major virulence factor of this necrotrophic pathogen. It is important to further investigate plant responses to this non-specific toxin. Therefore, in the present study, we compared the patterns of total soluble proteins and xylem morphology of partially resistant and susceptible sunflower lines after treatment with Sclerotinia culture filtrate. The basal stems of both lines were treated with 40 mM oxalic acid (pH 3.7) of fungus culture filtrate and samples were collected at 24, 48 and 72 hours post treatment. In SDS-PAGE protein pattern new protein bands appeared in both lines after treatment. These observations suggest induction of stress-related proteins upon culture filtrate treatment. The identities of these new proteins need to be more clarify in future investigations. The changes in xylem morphology and degree of lignification of both lines was studied by light microscopy and microtome sectioning techniques after treatment with S. sclerotiorum culture filtrate. Anatomical investigations revealed changes in xylem diameter and xylem lignification of treated lines at various time points. More lignin deposition in xylem vessels of partially resistant line has been observed after treatment. In addition, the size of xylem vessels in partially resistant line has been sharply decreased upon pathogen filtrate treatment. The results of this study will help us gain a more complete understanding of resistance mechanisms to this cosmopolitan and devastating pathogen.

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Section: Protein Patterns Of Total Proteins In Susceptible and Partiamentioning

confidence: 99%

Section: Changes In Xylem Vessels Of Sunflower Lines After Culture Fimentioning

confidence: 99%

Changes in Anatomical Features and Protein Pattern of Sunflower Partially Resistant and Susceptible Lines During Infection By Virulence Factors of Sclerotinia Sclerotiorum

Monazzah¹,

Enferadi

Rabiei³

et al. 2019

Phyton

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“…A few studies conducted in cultivated sunflower have exploited the advantages and sensitivity of this methodology to identify differentially-expressed genes under biotic or abiotic stress conditions 27,28 , and none of them addressed the S. sclerotiorum-sunflower interaction. Indeed, only two low-throughput transcriptomic studies have focused on this pathosystem 29,30 . In addition, most transcriptomic analyses of Sclerotinia infection were made with data obtained from symptomatic plants [20][21][22] .…”

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confidence: 99%

Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis

Fass

Rivarola

Ehrenbolger

et al. 2020

Sci Rep

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Sclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance are still largely unknown. Here, we investigated the Sclerotiniasunflower pathosystem by analysing temporal changes in gene expression in one susceptible and two tolerant inbred lines (IL) inoculated with the pathogen under field conditions. Differential expression analysis showed little overlapping among ILs, suggesting genotype-specific control of cell defense responses possibly related to differences in disease resistance strategies. Functional enrichment assessments yielded a similar pattern. However, all three ILs altered the expression of genes involved in the cellular redox state and cell wall remodeling, in agreement with current knowledge about the initiation of plant immune responses. Remarkably, the over-representation of long non-coding RNAs (lncRNA) was another common feature among ILs. Our findings highlight the diversity of transcriptional responses to SHR within sunflower breeding lines and provide evidence of lncRNAs playing a significant role at early stages of defense. Sunflower is one of the most important crops for the production of high-quality oil and seeds consumed by both humans and livestock. In recent years, sunflower production showed a steady increase driven by a boost in sunflower oil consumption (FAO, 2017). However, the projected expansion of the sunflower oil market requires appropriate agronomic management and improved genetic resources to cope with abiotic and biotic stresses. Among the latter, special attention should be paid to fungal diseases, as they have the greatest impact on yield and seed quality 1. The necrotrophic fungus Sclerotinia sclerotiorum is the causal agent of Sclerotinia head (SHR) and stalk (SSR) rots in sunflower. In particular, SHR is a recurrent disease in sunflower-growing areas worldwide. It affects oil quality and, under favourable conditions, may lead to total production loss 1,2. Chemical fungicides proved to be ineffective and breeding of resistant genotypes has emerged as the most promising control strategy 3. So far, there is no evidence of any major gene controlling the resistance to SHR in sunflower. Instead, inbred lines (ILs) show a broad range of responses in accordance with quantitative disease resistance (QDR) patterns depending on the genotype 4-8. During the last 20 years, QTL mapping techniques have been used to unravel the complexity of the defense response to both SHR and SSR in sunflower. Biparental mapping has led to the discovery of several main effect loci and epistatic interactions 9-11 , whereas association mapping has served to identify candidate genes

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Biochemical defense mechanism associated with host-specific disease resistance pathways against Rhizoctonia solani AG3-PT potatoes canker disease

Soheili-Moghaddam

Esfahani²,

Mousanejad³

et al. 2022

Planta

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Enzymatic activities and pathogenesis-related genes expression in sunflower inbred lines affected bySclerotinia sclerotiorumculture filtrate

Abstract: The time of antioxidant system activation in host is important in order to contribute to defence responses. To date, the changes in the expression of PR1 and PDF 1.2 and contribution of (1,4)-β-glucanase enzyme in sunflower defence responses were not reported in previous studies.

Cited by 6 publications

References 83 publications

Changes in Anatomical Features and Protein Pattern of Sunflower Partially Resistant and Susceptible Lines During Infection By Virulence Factors of Sclerotinia Sclerotiorum

Changes in Anatomical Features and Protein Pattern of Sunflower Partially Resistant and Susceptible Lines During Infection By Virulence Factors of Sclerotinia Sclerotiorum

Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis

Biochemical defense mechanism associated with host-specific disease resistance pathways against Rhizoctonia solani AG3-PT potatoes canker disease

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