Biochemical defense mechanism associated with host-specific disease resistance pathways against Rhizoctonia solani AG3-PT potatoes canker disease

Soheili-Moghaddam, Bita; Esfahani, Mehdi Nasr; Mousanejad, S.; Hassanzadeh-Khankahdani, Hamed; Karbalaei, Reza

doi:10.1007/s00425-022-04039-2

Cited by 11 publications

(7 citation statements)

References 73 publications

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“…For instance, the key enzymes of phenylpropanoid/ avonoid pathway consistent to our related enzymes; CAT, POD, and SOD, respectively (Qalavand et al, 2022;Soheili et al, 2022). There is enough evidence to show the vital role of signaling molecules and phytohormones in regulating defense responses in plants (Bauer et al, 2021;Soheili et al, 2023). For instant, there are reports indicating that after pathogen inoculation, the early accumulation of iso avonoid phytoalexins kievitone and phaseollidin was occurred in FOC-treated plants (Marín-Pimentel et al, 2023).…”

Section: Discussionsupporting

confidence: 80%

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Poursakhi,

Asadi-Gharneh,

Esfahani

et al. 2024

Preprint

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Fusarium basal rot (FBR) is a serious disease of onion that causes significant losses globally. There are several control measures, of which planting of resistant FBR-cultivars is the most advantageous control option. Thus, to elucidate the involving mechanisms to FBR infection, we consequently carried out quantification changes in antioxidant enzymes activity including ROS (reactive oxygen species); superoxide dismutase (SOD), peroxidase (POX), and catalase (CAT) in two resistant onion genotypes ‘Saba’ and ‘Saba – HS’; and susceptible ‘Savannah Sweet’ and ‘Golden eye’ following inoculation with FBR agent. As a complimentary to the related antioxidant enzymes, we also characterized the critical transcription factors (TFs); R1, R5 and RGA29 marker genes using qRT-PCR involved in endowing resistance at three time course/week post inoculation (wpi). Additionally, we analyzed the changes in biomass growth parameters (BGPs) accordingly. The results showed that the lowest disease severity percentage (DSP) was in resistant ‘Saba’ and ‘Saba – HS’ (6.7 and 4.7 %), and the highest ones in susceptible ‘Savannah Sweet’ and ‘Golden eye’ (88.9 and 89.6%), respectively. There was also a significant increase in CAT, POX, and SOD activities in resistant genotypes. The highest CAT activity was recorded in resistant ‘Saba’- 4.91, and Saba-HS - 3.75 fold, followed by the same trend relatively for POX (‘Saba’- 3.53 and ‘Saba-HS’- 2.35 fold); and SOD (‘Saba’ 14.46 -fold and ‘Saba-HS’ 12.95 –fold) significantly. It was also found that FOC could up-regulate the defense marker genes; R1, PR5, and RGA29 expression in resistant '‘Saba’' and ‘Saba’-HS ranging from 1.23 to 5.01-fold. The highest regulation was in PR5-5.01 fold followed by R1-4.78; and RGA29-3.83-fold, respectively. In addition, BGPs displayed almost similar trends as was in enzyme and gene expression accordingly. These findings lead to introduction of newly FBR-resistant onion genotypes, and also can be implicated in integrated pest management planning programs against FBR disease. Cumulatively, the proteomic-transcriptomic-data provide novel-insights for improving onion-breeding programs against FBR disease.

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Section: Discussionsupporting

confidence: 80%

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Poursakhi,

Asadi-Gharneh,

Esfahani

et al. 2024

Preprint

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“…Root tissues were collected at different time points after infection. Antioxidant enzyme activities were analyzed according to Zhuo et al [ 41 ] and Soheili-Moghaddam et al [ 42 ]. There are three biological replicates of each sample.…”

Section: Methodsmentioning

confidence: 99%

Screening of Alfalfa Varieties Resistant to Phytophthora cactorum and Related Resistance Mechanism

Tao

Zhao

et al. 2023

Plants

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Alfalfa is one of the most important legume forages in the world. Root rot caused by soil-borne pathogens severely restricts the production of alfalfa. The knowledge of the interaction between alfalfa and root rot-pathogens is still lacking in China. Phytophthora cactorum was isolated from symptomatic seedlings of an alfalfa field in Nanjing with high levels of damping-off. We observed the different infection stages of P. cactorum on alfalfa, and found that the purified P. cactorum strain was aggressive in causing alfalfa seed and root rot. The infecting hyphae penetrated the epidermal cells and wrapped around the alfalfa roots within 48 h. By evaluating the resistance of 37 alfalfa cultivars from different countries to P. cactorum, we found Weston is a resistant variety, while Longdong is a susceptible variety. We further compared the activities of various enzymes in the plant antioxidant enzyme system between Weston and Longdong during P. cactorum infection, as well as gene expression associated with plant hormone biosynthesis and response pathways. The results showed that the disease-resistant variety Weston has stronger antioxidant enzyme activity and high levels of SA-responsive PR genes, when compared to the susceptible variety Longdong. These findings highlighted the process of interaction between P. cactorum and alfalfa, as well as the mechanism of alfalfa resistance to P. cactorum, which provides an important foundation for breeding resistant alfalfa varieties, as well as managing Phytophthora-caused alfalfa root rot.

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“…The mechanism of antioxidant activity of vitamin C in the treatment of diabetes mellitus (DM) is through its intracellular action (49,21). This defense mechanism relies on the presence of antioxidant enzymes, such as superoxide dismutase (SOD), catalases (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), which catalyze the decomposition of reactive oxygen species (ROS) and maintain redox homeostasis.…”

Section: Antioxidant Enzymes Of Ethanolic Extract Of Cspicatusmentioning

confidence: 99%

Phytochemical profiling and In-vitro antioxidant activity of In-vivo α- amylase and α- glucosidase inhibitory activities of kidney Costus spicatus extract in diabetic Albino wistar Rats

Sivalingam

Pandian

Rengarajan

et al. 2023

Preprint

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The study found that C. spicatus extract had a beneficial effect on body weight and blood glucose levels in diabetic rats. Additionally, the extract contained polyphenol compounds with antioxidant, enzyme inhibition, and antiglycation activity. The blood glucose levels of the experimental groups were measured over a 28-day period, and the lipid profile was also assessed. The important result of this study is that the plant extract from C.spicatus was able to recover the antioxidant activity parameters, as well as improve the activity of glycolysis enzymes in the kidney of diabetic rats. This suggests that the plant extract has potential as a natural remedy for diabetes and its complications. Diabetic control + C.spicatus 500 mg/kg at 0.99 ± 0.05ab, Pyruvate kinase as a normal control at 20.01 ± 0.05a and 14.41 ± 1.2c, and Glucose-6-Phosphatase Dehydrogenase as a normal control at 0.52 ± 0.02a and Diabetic control + C.spicatus 500 mg/kg at 0.66 ± 0.05c. The study found that the C. spicatus leaf extract was effective in improving the antioxidant activity of important parameters such as SOD, CAT, GPx, and GR, and protected against hyperglycemia and oxidative damage in type 2 diabetes-induced hepatorenal functions both in vitro and in vivo. The kidney glycolysis enzymes, Hexokinase, Pyruvate kinase, and Glucose-6-Phosphatase Dehydrogenase, also showed improvement in the experimental group. Overall, the C. spicatus leaf extract proved to be effective in protecting against hyperglycemia and oxidative damage in type 2 diabetes-induced hepatorenal functions in both in vitro and in vivo studies.

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Biochemical defense mechanism associated with host-specific disease resistance pathways against Rhizoctonia solani AG3-PT potatoes canker disease

Cited by 11 publications

References 73 publications

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot

Screening of Alfalfa Varieties Resistant to Phytophthora cactorum and Related Resistance Mechanism

Phytochemical profiling and In-vitro antioxidant activity of In-vivo α- amylase and α- glucosidase inhibitory activities of kidney Costus spicatus extract in diabetic Albino wistar Rats

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