Environmental factors modulate biofilm formation by <i>Staphylococcus aureus</i>

Liu, Ying; Zhang, Jiang; Ji, Yinduo

doi:10.1177/0036850419898659

Cited by 34 publications

(40 citation statements)

References 77 publications

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“…Different environmental factors modulate biofilm formation by S. aureus 16 . During infection, certain pharmacological agents or their derivatives may become a signal for bacteria to promote regulatory changes, and consequently alter gene expression thus allowing S. aureus adaptation to persist in the host.…”

Section: Introductionmentioning

confidence: 99%

Salicylic acid stabilizes Staphylococcus aureus biofilm by impairing the agr quorum-sensing system

Dotto

Serrat

Ledesma

et al. 2021

Sci Rep

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Salicylic acid (SAL) has recently been shown to induce biofilm formation in Staphylococcus aureus and to affect the expression of virulence factors. This study was aimed to investigate the effect of SAL on the regulatory agr system and its impact on S. aureus biofilm formation. The agr quorum-sensing system, which is a central regulator in S. aureus pathogenicity, plays a pivotal role in the dispersal of S. aureus mature biofilms and contributes to the creation of new colonization sites. Here, we demonstrate that SAL impairs biofilm dispersal by interfering with agr expression. As revealed by our work, protease and surfactant molecule production is diminished, and bacterial cell autolysis is also negatively affected by SAL. Furthermore, as a consequence of SAL treatment, the S. aureus biofilm matrix revealed the lack of extracellular DNA. In silico docking and simulation of molecular dynamics provided evidence for a potential interaction of AgrA and SAL, resulting in reduced activity of the agr system. In conclusion, SAL stabilized the mature S. aureus biofilms, which may prevent bacterial cell dissemination. However, it may foster the establishment of infections locally and consequently increase bacterial persistence leading to therapeutic failure.

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Section: Introductionmentioning

confidence: 99%

Salicylic acid stabilizes Staphylococcus aureus biofilm by impairing the agr quorum-sensing system

Dotto

Serrat

Ledesma

et al. 2021

Sci Rep

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“…Their implantation leads to a host biological response: chronic inflammation, foreign-body response, and host-protein adsorption like fibronectin cover, which also facilitates biofilm development ( Fischer et al, 1996 ; Gibon et al, 2017a , b ; Masters et al, 2019 ). According to the infection site, the proportion of viable adherent bacteria, dead cells, and matrix within the biofilm could be very variable ( Nandakumar et al, 2013 ; Liu et al, 2020 ). Focusing on bone context, primary periprosthetic joint infections (PJIs) after surgery represent approximately 2% of total joint arthroplasty ( Grammatico-Guillon et al, 2012 ; Springer et al, 2017 ; Shoji and Chen, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment

et al. 2021

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Staphylococcus aureus species is an important threat for hospital healthcare because of frequent colonization of indwelling medical devices such as bone and joint prostheses through biofilm formations, leading to therapeutic failure. Furthermore, bacteria within biofilm are less sensitive to the host immune system responses and to potential antibiotic treatments. We suggested that the periprosthetic bone environment is stressful for bacteria, influencing biofilm development. To provide insights into S. aureus biofilm properties of three strains [including one methicillin-resistant S. aureus (MRSA)] under this specific environment, we assessed several parameters related to bone conditions and expected to affect biofilm characteristics. We reported that the three strains harbored different behaviors in response to the lack of oxygen, casamino acids and glucose starvation, and high concentration of magnesium. Each strain presented different biofilm biomass and live adherent cells proportion, or matrix production and composition. However, the three strains shared common responses in a bone-like environment: a similar production of extracellular DNA and engagement of the SOS response. This study is a step toward a better understanding of periprosthetic joint infections and highlights targets, which could be common among S. aureus strains and for future antibiofilm strategies.

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“…Comparing the effect of sugars on the formation of biofilms by strains of S. aureus (Voronkova & Shevchenko, 2017) and S. epidermidis, the following can be noted: the general features of the effect of sugars on strains of both species were a pronounced inhibitory effect at concentrations of 2.5% or more, and an almost neutral reaction at concentration of 0.5%. In experimental research (Liu et al, 2020) it was shown that at concentration of glucose 0.5% with presence of 3.0% NaCl in trypticase soy broth medium for different S. aureus strains variable capacities to form biofilms were observed, which indicates the role of genetic background in biofilm formation. However, there is a tendency to increase in biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

Influence of sugars on biofilm formation of Staphylococcus epidermidis

Vashchenko

Voronkova

Kulyk

et al. 2021

Regul. Mech. Biosyst.

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The problem of biofilm formation by clinical strains of opportunistic bacteria is one of the most significant for medicine, because in a state of biofilm bacteria become more resistant to environmental factors, including antibiotics, a situation that can cause failure of treatment. Among opportunistic pathogens staphylococci are of special interest. Knowledge about the peculiarities of biofilm formation of these strains, in particular the polysaccharide biosynthesis, can be used for creation of a strategy of prophylaxis of different lesions that bind with staphylococci. The effect of different concentrations of the most widespread sugars (glucose, sucrose, lactose, galactose) on the activity of biofilm formation by strains of Staphylococcus epidermidis was investigated. Strains of S. epidermidis (n = 7) were isolated from the reproductive tract of women with dysbiosis. The cultures were grown in universal synthetic media with concentration of one of the listed sugars (0.5–3.0%) during 72 h. Results were obtained colorimetrically. We studied the number of cells in biofilm and the index of biofilm formation. The largest number of cells in the biofilm was observed when the culture incubated in a medium with 2.0% of glucose (increase of 25.3 times compared to control). The amount of CFU in the control biofilm was 9.96 lg CFU/mL. The glucose concentration of 3.0% inhibited the biofilm formation: the number of cells in the biofilm was 569 times less compared to the control. The highest value of biofilm formation index was 7.2, which was 1.3 times higher than the control (5.4). In the presence of lactose and galactose in nutrient medium in concentrations from 1.0% a decrease in the number of cells and biofilm formation index were observed. The received data show that process of biofilm formation is significantly dependent on external sources of sugars, which can indicate the possibility of their use as antibiofilm drug compounds, which inhibit membrane transport of sugars in bacteria.

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Environmental factors modulate biofilm formation by Staphylococcus aureus

Cited by 34 publications

References 77 publications

Salicylic acid stabilizes Staphylococcus aureus biofilm by impairing the agr quorum-sensing system

Salicylic acid stabilizes Staphylococcus aureus biofilm by impairing the agr quorum-sensing system

Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment

Influence of sugars on biofilm formation of Staphylococcus epidermidis

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