Salicylic acid stabilizes Staphylococcus aureus biofilm by impairing the agr quorum-sensing system

Dotto, Cristián; Serrat, Andrea Lombarte; Ledesma, Martín; Vay, Carlos; Ehling‐Schulz, Monika; Sordelli, D O; Grunert, Tom; Buzzola, Fernanda R.

doi:10.1038/s41598-021-82308-y

Cited by 34 publications

(31 citation statements)

References 64 publications

(92 reference statements)

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“…A previous report demonstrated that deletion of multiple lytic genes, such as prophages in Gram-positive bacteria, e.g., Bacillus subtilis , can prevent cell lysis and enhance biomass 80 . A recent study showed that the salicylic acid interferes with the regulatory agar QS system in pathogenic Staphylococcus aureus , consequently negatively affecting autolysis 58 . The delay of autolysis in DT mutants of DSM 7029 was probably due to the deletion of lytic genes or their regulatory genes during the genome reduction procedure, e.g., lysozyme or XRE family transcriptional regulator-coding genes (Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria

et al. 2021

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Heterologous expression of biosynthetic gene clusters (BGCs) avails yield improvements and mining of natural products, but it is limited by lacking of more efficient Gram-negative chassis. The proteobacterium Schlegelella brevitalea DSM 7029 exhibits potential for heterologous BGC expression, but its cells undergo early autolysis, hindering further applications. Herein, we rationally construct DC and DT series genome-reduced S. brevitalea mutants by sequential deletions of endogenous BGCs and the nonessential genomic regions, respectively. The DC5 to DC7 mutants affect growth, while the DT series mutants show improved growth characteristics with alleviated cell autolysis. The yield improvements of six proteobacterial natural products and successful identification of chitinimides from Chitinimonas koreensis via heterologous expression in DT mutants demonstrate their superiority to wild-type DSM 7029 and two commonly used Gram-negative chassis Escherichia coli and Pseudomonas putida. Our study expands the panel of Gram-negative chassis and facilitates the discovery of natural products by heterologous expression.

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Section: Discussionmentioning

confidence: 99%

Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria

et al. 2021

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“…To verify the effect of boron derivative on biofilm formation, the growth curve of recombinant E. coli was detected ( Figure 2 a). This addition had no effect on the strain’s growth, which had reached a stable phase after 15 h. Therefore, the period of the highest biofilm index of recombinant E. coli was at the initial stage of stable growth of the strain, which corresponded to the period with the highest AI-2 activity in previous studies [ 25 ]. Figure 2 b illustrates the growth of recombinant E. coli biofilm with H 3 BO 3 , NaBO 2 , and NaBO 3 .…”

Section: Resultsmentioning

confidence: 76%

Boron Derivatives Accelerate Biofilm Formation of Recombinant Escherichia coli via Increasing Quorum Sensing System Autoinducer-2 Activity

Chen

Yan

Zhan

et al. 2022

IJMS

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Boron is an essential element for autoinducer-2 (AI-2) synthesis of quorum sensing (QS) system, which affects bacterial collective behavior. As a living biocatalyst, biofilms can stably catalyze the activity of intracellular enzymes. However, it is unclear how boron affects biofilm formation in E. coli, particularly recombinant E. coli with intracellular enzymes. This study screened different boron derivatives to explore their effect on biofilm formation. The stress response of biofilm formation to boron was illuminated by analyzing AI-2 activity, extracellular polymeric substances (EPS) composition, gene expression levels, etc. Results showed that boron derivatives promote AI-2 activity in QS system. After treatment with H3BO3 (0.6 mM), the AI-2 activity increased by 65.99%, while boron derivatives increased the biomass biofilms in the order H3BO3 > NaBO2 > Na2B4O7 > NaBO3. Moreover, treatment with H3BO3 (0.6 mM) increased biomass by 88.54%. Meanwhile, AI-2 activity had a linear correlation with polysaccharides and protein of EPS at 0–0.6 mM H3BO3 and NaBO2 (R2 > 0.8). Furthermore, H3BO3 upregulated the expression levels of biofilm formation genes, quorum sensing genes, and flagellar movement genes. These findings demonstrated that boron promoted biofilm formation by upregulating the expression levels of biofilm-related genes, improving the QS system AI-2 activity, and increasing EPS secretion in E. coli.

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“…Colony spreading of E. coli was performed as described in 20,21 . Isolates were grown in Luria-Bertani broth for 24 hours.…”

Section: Colony Spreading Of E Colimentioning

confidence: 99%

Isolation and identification of Biofilm-producing E. coli from drinking water.

Fatima

Mirani

Siddiqui

et al. 2022

Int. j. endorsing health sci. res.

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Background: The increasing rate of water-borne diseases in Karachi demands the characterization of associated pathogens. Nowadays, water-borne infections become more resistant to treatment due to biofilm formation and excessive use of antibiotics. Biofilm formation in water deteriorates the quality of water. This study identified and characterized biofilm-producing E. coli in drinking water. Methodology: Water samples were analyzed for heterotrophic plate count and total coliforms, fecal coliforms, and E. coli. The Kirby disk bar diffusion method was used to investigate antibiotic susceptibility in biofilm-producing E. coli. The Congo red and tube ring methods were used to identify biofilm producers. The effect of biofilm formation on the hydrophobicity of E. coli was performed by the BATH method. The soft agar method determines the colony spreading ability of biofilm and non-biofilm producers. Molecular characterization of virulence genes of E. coli was performed by a PCR. Scanning electron microscopy for biofilm construction was conducted. Results: The total 120 water samples were tested for heterotrophic plate count and total coliforms, fecal coliforms, and E. coli. 78% were unfit in this study, and 21.66% were fit. 38 E. coli strains were found in water samples. According to findings, the hydrophobicity of biofilm-producing isolates increased with the incubation period. Colony-forming unit drops one logarithm in the biofilm state compared to the planktonic stage. Biofilm producers were more resistant to antibiotics. The virulence genes, pet, lt, and stx2, were used for the molecular characterization. Conclusion: The presence of biofilm-producing E. coli in drinking water is alarming, and it indicates inappropriate treatment of the water supply system. To prevent rapid water-borne diseases, adequate actions are required to control drinking-water biofilm producers.

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Salicylic acid stabilizes Staphylococcus aureus biofilm by impairing the agr quorum-sensing system

Cited by 34 publications

References 64 publications

Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria

Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria

Boron Derivatives Accelerate Biofilm Formation of Recombinant Escherichia coli via Increasing Quorum Sensing System Autoinducer-2 Activity

Isolation and identification of Biofilm-producing E. coli from drinking water.

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