Boron Derivatives Accelerate Biofilm Formation of Recombinant Escherichia coli via Increasing Quorum Sensing System Autoinducer-2 Activity

Chen, Huan; Yan, Chenghai; Zhan, Yu-Fan; Geng, Li-Tian; Zhu, Linlin; Gong, Lu-Chan; Wang, Jun

doi:10.3390/ijms23158059

Cited by 7 publications

(6 citation statements)

References 51 publications

(51 reference statements)

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“…The biofilm biomass of E. coli was determined using crystal violet as reported previously [ 5 , 45 ]. The seed liquid of the two strains with a concentration of 10 7 CFU/mL was inoculated into a 96-well plate, and it was cultured at 37 °C for 24 h. Each group was set with 6 wells as parallel, and the wells without inoculated strains were regarded as a negative control.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, 4% glutaraldehyde was added to a 12-well plate and placed in a refrigerator at 4 °C overnight. Then, the 4% glutaraldehyde was removed, and the biofilm adsorbed on the glass slide was stained with SYBR Green I dye for 30 min, which was used for fluorescence inversion microscopy (Eclipse TS100, Nikon, Tokyo Metropolis, Japan) and laser focusing scanning microscopy (LEXTOLS4000, Olympus, Tokyo, Japan) observation according to the method of Chen and Zhu et al [ 5 , 50 ]. In addition, ethanol was used to dehydrate the biofilm fixed with 4% glutaraldehyde and freeze-dried for scanning electron microscope (Regulus-8100, Hitachi, Tokyo, Japan) observation [ 51 ].…”

Section: Methodsmentioning

confidence: 99%

“…Biofilms are not only simple bacterial community, but also involve necessary physiological and phenotypic changes. It is a highly structured multicellular microbial community, which is composed of bacterial cells and an extracellular polymeric substance (EPS) matrix, which could provide niches and enhance stress resistance [ 5 ]. With an in-depth understanding of the formation mechanism, the advantages of biofilms in beneficial bacteria have been gradually discovered and have played an important role in environmental restoration, sewage treatment and biocatalysis [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

Yan,

Chen,

Yang

et al. 2023

IJMS

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The high cell density, immobilization and stability of biofilms are ideal characteristics for bacteria in resisting antibiotic therapy. CsgD is a transcription activating factor that regulates the synthesis of curly fimbriae and cellulose in Escherichia coli, thereby enhancing bacterial adhesion and promoting biofilm formation. To investigate the role of CsgD in biofilm formation and stress resistance in bacteria, the csgD deletion mutant ΔcsgD was successfully constructed from the engineered strain E. coli BL21(DE3) using the CRISPR/Cas9 gene-editing system. The results demonstrated that the biofilm of ΔcsgD decreased by 70.07% (p < 0.05). Additionally, the mobility and adhesion of ΔcsgD were inhibited due to the decrease in curly fimbriae and extracellular polymeric substances. Furthermore, ΔcsgD exhibited a significantly decreased resistance to acid, alkali and osmotic stress conditions (p < 0.05). RNA-Seq results revealed 491 differentially expressed genes between the parent strain and ΔcsgD, with enrichment primarily observed in metabolism-related processes as well as cell membrane structure and catalytic activity categories. Moreover, CsgD influenced the expression of biofilm and stress response genes pgaA, motB, fimA, fimC, iraP, ompA, osmC, sufE and elaB, indicating that the CsgD participated in the resistance of E. coli by regulating the expression of biofilm and stress response. In brief, the transcription factor CsgD plays a key role in the stress resistance of E. coli, and is a potential target for treating and controlling biofilm.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

Yan,

Chen,

Yang

et al. 2023

IJMS

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“…Addition of various boron derivatives (H 3 BO 3 , NaBO 2 , Na 2 B 4 O 7 , and NaBO 3 ) to E. coli culture medium, stimulated the biofilm formation by increasing AI-2 QS system activity. [16] AI-2 is synthesized from 4,5-dihydroxy-2,3-pentanedione (DPD) via cyclized form pro-AI-2. Although reaction of pro-AI-2 with borate (B(HO) 4 À ) produces AI-2, pro-AI-2 can give other related diester signaling molecules if boron is depleted.…”

Section: Introductionmentioning

confidence: 99%

Development of a Highly Boron Tolerant Pseudomonas sp.−Fe‐MOF Bioanode

Avcı,

Sezer Kürkçü,

Çöl

et al. 2023

ChemistrySelect

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In this work, a bioanode which was based on carbon felt electrode (CFE) that contained highly boron tolerant bacterium Pseudomonas sp. isolated from a boron mine (Pseudomonas isolate BC4B) and iron‐metal organic framework (Fe‐MOF) was developed. To the best of our knowledge, this is the first study in which a highly boron tolerant bacterium, Pseudomonas isolate BC4B was investigated as a bioanode material. It was observed that Fe‐MOF increased the signal significantly by behaving as a catalyst. During the study, the optimization of Fe‐MOF amount, bacteria amount and substrate concentration were done. Also, the effect of different substrate amounts on the bioanode electrochemical signal was investigated. Consequently, the highest current density value was obtained with 5 mM substrate amount. Meanwhile, in order to investigate the reproducibility of developed bioanode, relative standard deviation value was calculated and found as 1.39 % (n : 3) for 4 mM concentration of H3BO3.

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“…These authors found that adhesion to the oral environment cannot be controlled by structural means. Chen et al [8] found that boron (an essential element for autoinducer-2 synthesis of quorum sensing systems) promoted biofilm formation by upregulating the expression levels of biofilm-related genes, improving the autoinducer-2 activity and increasing the secretion of extracellular polymeric substances by Escherichia coli.…”

mentioning

confidence: 99%

Contribution to Understanding the Mechanisms Involved in Biofilm Formation, Tolerance and Control

Simões

2023

IJMS

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Biofilms constitute a protected mode of growth that allows the colonizing microbial cells to survive in hostile environments, even when an antimicrobial agent is present. The scientific community has come to understand many things about the growth dynamics and behavior of microbial biofilms. It is now accepted that biofilm formation is a multifactorial process that starts with the adhesion of individual cells and (auto-)coaggregates of cells to a surface. Then, attached cells grow, reproduce and secrete insoluble extracellular polymeric substances. As the biofilm matures, biofilm detachment and growth processes come into balance, such that the total amount of biomass on the surface remains approximately constant in time. The detached cells retain the phenotype of the biofilm cells, which facilitates the colonization of neighboring surfaces. The most common practice to eliminate unwanted biofilms is the application of antimicrobial agents. However, conventional antimicrobial agents often show inefficacy in the control of biofilms. Much remains to be understood in the biofilm formation process and in the development of effective strategies for biofilm prevention and control. The articles contained in this Special Issue deal with biofilms of some important bacteria (including pathogens such as Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) and fungi (Candida tropicalis), providing novel insights into their formation mechanisms and implications, together with novel methods (e.g., use of chemical conjugates and combinations of molecules) that can be used to disrupt the biofilm structure and kill the colonizing cells.

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Boron Derivatives Accelerate Biofilm Formation of Recombinant Escherichia coli via Increasing Quorum Sensing System Autoinducer-2 Activity

Cited by 7 publications

References 51 publications

The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses

Development of a Highly Boron Tolerant Pseudomonas sp.−Fe‐MOF Bioanode

Contribution to Understanding the Mechanisms Involved in Biofilm Formation, Tolerance and Control

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