Enumeration of Total Coliforms and E. coli in Foods by the SimPlate® Coliform and E. coli Color Indicator Method and Conventional Culture Methods: Collaborative Study

Abstract

The relative effectiveness of the SimPlate® Coliform and E. coli Color Indicator (CEc-CI) method was compared to the AOAC 3-tube Most Probable Number (MPN) methods for enumerating and confirming coliforms and Escherichia coli in foods (966.23 and 966.24). In this study, test portions were prepared and analyzed according to the conditions stated in both the AOAC methods and SimPlate directions for use. Six food types were artificially contaminated with coliform bacteria and E. coli: frozen burritos, frozen broccoli, fluid pasteurized milk, whole almond nut meats, cheese, and powdered cake mix. Method comparisons were conducted. Overall, the SimPlate method demonstrated <0.3 log difference for total coliform and E. coli counts compared to the AOAC reference methods for the majority of food types and levels analyzed. In all cases, the repeatability and reproducibility of the SimPlate CEc-CI method were not different from those of the reference methods and in certain cases, were statistically better than those of the AOAC 3-tube MPN methods. These results indicate that the SimPlate CEc-CI method and the reference culture methods are comparable for enumeration of both total coliforms and E. coli in foods.

Use of Propolis in the Sanitization of Lettuce

“…Enumeration of coliforms and E. coli was done using the SimPlate CEc-CI method [ 38 ] with multiple test medium (BioControl System, Bellevue, WA, USA), according to the manufacturer’s instructions and previously procedures of [ 39 ]. One milliliter from the basic dilution was placed in the center of the SimPlate plating device, and 9 mL of a mixed nutrient agar with blue color was added at the same spot.…”

COMPARISON OF CONVENTIONAL PLATING AND SIMPLATE METHODS FOR ENUMERATION OF AEROBIC MICROORGANISMS, COLIFORM AND ESCHERICHIA COLI IN FARM ENVIRONMENTAL SAMPLES

“…The plates were incubated at 35C for 24 h prior to counting coliforms (purple‐red colonies) and E. coli (fluorescent colonies under long‐wave UV light). Enumeration of coliforms and E. coli using the SimPlate CEc‐CI method with multiple test medium (BioControl System) was performed according to the manufacturer's instructions and procedures of Feldsine et al. (2005).…”

“…1998; Russel 2000; Lee and Lewis 2003). The SimPlate CEc‐CI method has recently been approved by the Research Institute of the AOAC, Method 2005.3 (Feldsine et al. 2005).…”

Caracterizações microbiológica e físico-química de pólens armazenados por abelhas sem ferrão

“…No estudo dos indicadores de qualidade sanitária, a determinação de Staphylococcus coagulase positiva foi realizada em Agar Baird Parker suplementado com gema de ovo e telurito de potássio 1% (Himedia, Índia) por 24 h a 37 °C. A quantificação de coliformes totais e Escherichia coli foi realizada com um Kit Simplate da Bio Control (FELDSINE et al, 2005). Utilizou-se a tabela Braz.…”