Dalbergia ecastaphyllum is a native Brazil plant with importance for beekeeping, and widely used in folk medicine. For the first time, the extracts of this plant were assessed for the presence of hydrophilic and lipophilic antioxidants, as well as inhibition of tyrosinase, free radicals scavenging and sunscreen protection. The antioxidant activity was evaluated by free radical scavenging (DPPH) and β-carotene bleaching assay. The tyrosinase inhibitory activity was evaluated and calculated the EC50. The photoprotective activity was measured using different concentrations of D. ecastaphyllum extracts. The Sun Protection Factor (SPF) of the samples was higher than 6, and the sample from Ilhéus showed the most pronounced photoprotective effect. Sample from Canavieiras presented the highest antioxidant activity by free radical scavenging DPPH and β-carotene bleaching method, with 92.41% and 48.34%, respectively. All samples inhibited the tyrosinase, especially the sample from Prado that was most effective (124.62 μg.mL-1). Significant negative correlation was found between flavonoid contents and inhibition of tyrosinase. The overall results provide relevant information about the Dalbergia ecastaphyllum species, indicating as potential material to cosmetic and pharmaceutical industry.
This study compared the nutraceutical potential of Apis mellifera and Melipona quadrifasciata anthidioides honeys from the semiarid region of Bahia, Brazil, using microbiological, melissopalynological, and physicochemical techniques. Total phenols and flavonoids and the antioxidant activity were also determined. All samples had good microbiological quality and safety with an absence of coliforms, Escherichia coli, Salmonella spp., Staphylococcus coagulase positive and Clostridium sulphite reducing spores. Were identified 41 pollen types belonging to 23 botanical families. Myrtaceae, Anacardiaceae, and Sapindaceae were the predominant pollen types in A. mellifera honey, while honey Malvaceae was the most representative in the M. q. anthidioides. Regarding the physicochemical parameters evaluated, 75% complied with the standards established by the Brazilian and European quality legislation. The honey samples of A. mellifera had higher values of phenolic compounds and flavonoids (260.0 to 341.51 mgGAE.kg À1 and from 114.44 to 216.29 mgQE.kg À1 , respectively). The samples from M. q. anthidioides presented higher antioxidant activity. The honeys of A. mellifera and M. q. anthidioides from the semiarid regions presented distinct botanical compositions, suggesting that both species use different plant sources, which possibly influenced the parameters related to honey quality as well as the content of phenolic compounds.
The aim of this study was to evaluate the influence of lyophilization and drying in stove on phenolic compounds content and the biological activity of Apis mellifera and Trigona spinipes pollens produced in Brazil. In general, the bee pollen produced by T. spinipes presented highest antioxidant activity in dried and fresh samples assessed either by test of plasma ferric reduction capacity (FRAP) and free radical scavenging assay (DPPH) methods. For A. mellifera bee pollen the antioxidant activity was higher on the fresh samples. Nevertheless, b-carotene bleaching assay (BCB) and linoleic acid content were higher in T. spinipes samples, mainly in the fresh ones. Higher antioxidant activity was owing to higher content in phenolic compounds. Lyophilization method was the best for phenolic compounds' conservation for both species. The bee pollen of both species has a high amount of flavonoids: kaempferol-3-O-glucoside was the most abundant in A. mellifera while for T. spinipes the most prevalent was resorcylic acid þ epicatechin. All extracts presented antibacterial activity against Saphylococcus aureus (ATCC 43300) TM , (ESA 83138150), (ESA 32), Pseudomonas aeruginosa (ATCC 15442) TM , Pseudomonas aeruginosa (MRC.4) and (MRC.10). The methods used for storage influenced the biological properties of bee pollen from both species. Regarding the content of phenolic compounds, differences were observed amongst the pollen types: for A. mellifera these were best preserved with lyophilization, while for T. spinipes the three storage methods were equivalent.
Resumo O estudo foi realizado com o objetivo de verificar tanto a condição microbiológica quanto as diferenças físico-químicas existentes em pólens armazenados por diferentes espécies de abelhas sem ferrão no Nordeste do Brasil. Os pólens armazenados foram coletados nas colônias mantidas em meliponários das seguintes espécies: Melipona subnitida, M.scutellaris, M. mandacaia, Scaptotrigona sp. e Frieseomellita varia. Com relação às análises de qualidade microbiológica, as mesmas foram realizadas em micro-organismos indicadores de qualidade comercial (mesófilos, bolores e leveduras), qualidade sanitária (Staphylococcus coagulase positiva, coliformes fecais e Escherichia coli), e de segurança (esporos de Clostridium sulfito redutores e Salmonella spp.). Para os parâmetros físico-químicos, os mesmos foram avaliados de acordo com a exigência do Regulamento Técnico (RT) de qualidade para pólen apícola, e também para compostos fenólicos. Todas as amostras apresentaram condição microbiológica de acordo com as recomendações aceitáveis para o consumo humano. Os resultados confirmaram as diferenças na composição físico-química do pólen armazenado das cinco espécies; no entanto, houve formação de três grupos de pólens armazenados com base nos parâmetros físico-químicos. As amostras da espécie M. scutellaris apresentaram conteúdo de compostos fenólicos cinco vezes mais elevado aos outros méis das espécies avaliadas. Logo, este estudo permitiu conhecer a composição dos pólens armazenados por abelhas sem ferrão, por conseguinte reforçando a necessidade de reformular o Regulamento Técnico (RT) existente no Brasil para pólen apícola, de forma a abranger os pólens armazenados das espécies nativas.
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