Entrapment of Saccharomyces cerevisiae cells in u.v. crosslinked hydroxyethylcellulose/poly(ethylene oxide) double-layered gels

Velickova, Elena; Petrov, Petar; Tsvetanov, Christo B.; Kuzmanova, Slobodanka; Cvetkovska, Meri; Winkelhausen, Eleonora

doi:10.1016/j.reactfunctpolym.2010.09.004

Cited by 10 publications

(5 citation statements)

References 25 publications

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“…Moreover, it was established that the immobilization procedure employed preserves the cells viability and biodegradation capability. Similar results were described by Velickova et al (2010). The increased stability of the cells during the long time of use with preserved vitality and catabolic activity could be explained by the protecting effect of the matrix in which they were immobilized.…”

supporting

confidence: 84%

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Hristov

Christova

Kabaivanova

et al. 2016

Polish Journal of Microbiology

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A b s t r a c tThe capability of the biosurfactantproducing strain Rhodococcus wratislawiensis BN38 to mineralize both aromatic and aliphatic xeno biotics was proved. During semicontinuous cultivation 11 g/l phenol was completely degraded within 22 cycles by Rhodococcus free cells. Immobilization in a cryogel matrix was performed for the first time to enhance the biodegradation at multiple use. A stable simultaneous hydrocarbon biodegradation was achieved until the total depletion of 20 g/l phenol and 20 g/l nhexadecane (40 cycles). The alkanotrophic strain R. wratislawiensis BN38 preferably degraded hexadecane rather than phenol. SEM revealed well preserved cells entrapped in the heterogeneous supermacroporous structure of the cryogel which allowed unhindered mass transfer of xenobiotics. The immobilized strain can be used in real conditions for the treatment of contaminated industrial waste water. 288of materials due to their unique heterogeneous open porous structure, which significantly increases the equilibrium sorption properties and allows unhindered diffusion of solutes, nanoparticles and microparticles. Usually, cryogels possess spongylike structure of huge pores (50-200 µm) containing free water surrounded by thin walls and, therefore, they are often used for immo bilization of enzymes and cells by entrapment inside the channels of interconnected pores (Lozinsky et al., 2003).Many bacterial strains have been isolated with the abilities to degrade nhexadecane and phenol sepa rately, which are often used to represent the aliphatic and aromatic pollutant (Yordanova et al., 2009; Abdel Megeed et al., 2010;Tambekar et al., 2012). However, few strains have been reported to have the dual abilities (Sun et al., 2012).This paper aims to report the first study on simul taneous biodegradation of nhexadecane and phenol by Rhodococcus wratislawiensis BN38 immobilized in hydroxypropylcellulose/poly (Nisopropylacrylamide) cryogel matrix. Experimental Materials and MethodsMicroorganism, media and cultivation. The R. wrati slawiensis BN38, employed in this study was isolated from soil polluted with hydrocarbons by a standard enrichment technique (Tuleva et al., 2008 O, 0.2 and phenol and nhexadecane at 500 mg/l, unless otherwise mentioned, was used. The pH of the medium was adjusted to 7.0. The MSM was solidified as MSM phenol agar by addition of 1.8% agar when necessary. Cultures grown on MSM agar supplemented with 500 mg/l phenol were used to inoculate 500 ml Erlenmeyer flasks, containing 100 ml liquid MSM. At each cycle MSM was supplemented to 100 ml with fresh sterile medium without cell transfer and only phenol was added. Cultures were incubated during the longterm semicontinuous biodegradation processes while shaking (120 rpm) at 29°C. Inocula with cell density (OD 610 nm) of 0.4 were employed in the processes of biodegradation. The pH values were 6.7-6.8 all the time, due to the buffering activity of the nutrient medium used.Bacterial growth was assessed by determination of the optical density (OD 610 nm) of t...

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confidence: 84%

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Hristov

Christova

Kabaivanova

et al. 2016

Polish Journal of Microbiology

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“…Therefore, a mixed system is formed consisting of immobilized and free cells. The leakage of cells was significantly reduced by covering the HEC cryogel, containing S. cerevisiae cells, with an outer layer based on photocrosslinked poly(ethylene oxide) (PEO) hydrogel [30]. The PEO layer did not hinder the diffusion of nutrient and fermentation products.…”

Section: Applicationsmentioning

confidence: 99%

Cryogels via UV Irradiation

Petrov

Tsvetanov

2014

Polymeric Cryogels

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“…This method allows preparation of various biocompatible, biodegradable, and/or thermosensitive supermacroporous cryogels by irradiation of moderately frozen aqueous systems with UV light (irradiation dose rate of 950 J m -2 s -1 ) for 2 -5 minutes in the presence of a photoinitiator. Such materials have been exploited for immobilization of yeast cells (Velickova et al, 2010), bacteria capable to degrade xenobiotics (Satchanska et al, 2009;Topalova et al, 2011), fi broblast cells (Petrov et al, 2011a), enzymes (Petrov et al, 2011b(Petrov et al, , 2012, and drugs Kostova et al, 2011).…”

Section: Bn10 Cells Entrapped In Cryogelsmentioning

confidence: 99%

“…1a). It has been established (Velickova et al, 2010) that the immobilization procedure employed in the present study preserves the cells' viability and biosynthetic capability. Considering both the thickness of cryogel walls (2 -3 μm) and the size of the cells (0.5 -0.7 μm), cells can be considered partly embedded in the polymer matrix (Fig.…”

Section: Infl Uence Of Carrier Type and Quantity On Biosurfactant Promentioning

confidence: 99%

Biosurfactant Production by Pseudomonas aeruginosa BN10 Cells Entrapped in Cryogels

Christova

Petrov

Kabaivanova

2013

Zeitschrift Für Naturforschung C

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Production of a rhamnolipid biosurfactant by cells of Pseudomonas aeruginosa strain BN10 immobilized into poly(ethylene oxide) (PEO) and polyacrylamide (PAAm) cryogels was investigated under semicontinuous shake fl ask conditions and compared to biosurfactant secretion by free cells. The biosurfactant synthesis was followed over 9 cycles of operation of the immobilized system, each cycle comprising 7 days at ambient temperature and neutral pH. Type and quantity of the carrier were optimized for the rhamnolipid production. The highest rhamnolipid yield of 4.6 g l-1 was obtained in the 6th cycle for the immobilized system with 3 g PEO compared to 4.2 g l-1 obtained for the free cells, thus immobilization provided physiological stability of the cells. Scanning electron microscopy revealed preservation of the cell shape and regular distribution of the cells under the matrix surface. The polymer matrices possessed chemical and biological stability and very good physico-mechanical characteristics which are a prerequisite for a high life span of these materials for the production of rhamnolipids

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Entrapment of Saccharomyces cerevisiae cells in u.v. crosslinked hydroxyethylcellulose/poly(ethylene oxide) double-layered gels

Cited by 10 publications

References 25 publications

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Cryogels via UV Irradiation

Biosurfactant Production by Pseudomonas aeruginosa BN10 Cells Entrapped in Cryogels

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