Biosurfactant Production by Pseudomonas aeruginosa BN10 Cells Entrapped in Cryogels

Christova, Nelly; Petrov, Petar; Kabaivanova, Lyudmila

doi:10.1515/znc-2013-1-207

Cited by 8 publications

(2 citation statements)

References 22 publications

(27 reference statements)

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“…18 Fabrication of CNT-cryogel composites with nanotubes embedded into the cryogel walls (top) and nanotubes deposited onto the inner surface of the cryogel (bottom). Reprinted from [27] with permission from Elsevier Production of a rhamnolipid biosurfactant by cells of Pseudomonas aeruginosa strain BN10 immobilized into PEO and PAAm cryogels was investigated under semicontinuous shake flask conditions and compared to biosurfactant secretion by free cells [33]. The yield of rhamnolipids in the immobilized system exceeded that of the free bacterial cells, distinguishing an effective bioprocess.…”

Section: Applicationsmentioning

confidence: 99%

Cryogels via UV Irradiation

Petrov

Tsvetanov

2014

Polymeric Cryogels

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Section: Applicationsmentioning

confidence: 99%

Cryogels via UV Irradiation

Petrov

Tsvetanov

2014

Polymeric Cryogels

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“…An important cost-reduction approach involves the immobilized cell method, where mi-croorganisms are retained or immobilized on solid supports or polymeric matrices, as opposed to the conventional free cell method. This immobilized cell technique is noteworthy for yielding enhanced chemical and biological stability, facilitating nal product recovery and extending the shelf life of microorganism strains, achieved through the reuse of strains across a greater number of production cycles (29).…”

Section: Introductionmentioning

confidence: 99%

Biosurfactant production by Pseudomonas: a systematic review

Araujo,

Santana,

Otenio

et al. 2024

Preprint

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It is of fundamental interest to research and develop innovative biotechnologies, as well as bioproducts that replace or are alternatives to those of non-renewable origin, such as biosurfactants in relation to traditional surfactants used in various sectors. Consequently, there are a large number of experimental studies addressing different subjects, especially with the use of bacteria of the genus Pseudomonas, however, there is a lack of work that demonstrates the evaluation of this science produced to date. Therefore, this article discusses the production of biosurfactants by Pseudomonas with the aim of surveying and analyzing experimental articles on this topic. To realize this, a systematic search was carried out with well-defined temporal space, databases and inclusion and exclusion criteria, based on metric studies that guided what information would be collected and the method of evaluation. Therefore, a large number of articles were selected, which demonstrated Pseudomonas aeruginosa as the bioagent mostly used in the tests, which aimed to improve the process in the area. Furthermore, interest in this field has increased over the years, predominantly in emerging market countries, where the most prominent authors on the topic are found. Therefore, it is necessary that there is an expansion of interest in the area to make the production of biosurfactants cheaper in areas that currently have greater development deficiencies, such as means of purifying the bioprocess and reducing foam formation in the bioprocess.

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Generation of biosurfactants by P. aeruginosa gi |KP163922| on waste engine oil in a free and immobilized cells system

Jujaru,

Pradhan,

Gaur

et al. 2024

Can J Chem Eng

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This study investigated biosurfactant production by the bacterial strain of P. aeruginosa gi |KP 163922| for a free and immobilized cells system using waste engine oil (WEO) as a substrate. The polyurethane foam (PUF) cubes (1 cm × 1 cm × 1 cm) were used as carriers for the immobilization. The batch experiments were performed in Erlenmeyer flasks and monitored at every 24‐h interval for both cell systems. The microbial population was counted using the plate count method, and the hydrocarbon degradation percentage was calculated to evaluate the bacterial activity. Surface tension was measured at regular intervals to ensure the presence of biosurfactants. The maximum reduction was 37 and 35 mN/m in a free and immobilized cells system. Immobilization of cells using PUF was found to be efficient in supporting bacterial growth, and after 48 h of incubation, the growth was 2.5 (±0.58) × 1011 CFU/mL. The chemical characterization using Fourier transform infrared (FTIR) spectroscopy confirmed the obtained product as rhamnolipid. Crude biosurfactant yield was found to be maximum in the case of the immobilized system, which was approximately 18 g/L. Scanning electron micrographs (SEM) of the used PUF cubes showed the strong adherence of biofilm to the cube surface and the potential of its reuse in multiple cycles. Gas chromatography–mass spectrometry (GC–MS) analysis confirms that the immobilized strain of P. aeruginosa exhibited superior biodegradation capabilities compared to free cells. Specifically, it was capable of reducing the concentration of polyaromatic hydrocarbons and converting more significant aliphatic compounds into metabolic byproducts such as alkanes, alkenes, cycloalkanes, and carbonyl groups.

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Biosurfactant Production by Pseudomonas aeruginosa BN10 Cells Entrapped in Cryogels

Cited by 8 publications

References 22 publications

Cryogels via UV Irradiation

Cryogels via UV Irradiation

Biosurfactant production by Pseudomonas: a systematic review

Generation of biosurfactants by P. aeruginosa gi |KP163922| on waste engine oil in a free and immobilized cells system

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