Entrapment of l-tryptophan producing Escherichia coli in different matrices: activity of immobilized cells

Langrené, Sylvie; Sicsic, Sames

doi:10.1016/0141-0229(84)90040-1

Cited by 7 publications

(2 citation statements)

References 11 publications

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“…Also it was found that indole addition near to the end of the logarithmic phase was more effective because L-Trp as well as other amino acids is produced in the stationary phase ( Figure 2 ). Langrene et al ( 33 ) used three-indolacrylic acid and observed an increased L-Trp production by ten folds. As the main result, L-Trp was produced from processed Orumiyeh beet molasses, as the L-Ser source.…”

Section: Discussionmentioning

confidence: 99%

Optimization of L-Tryptophan Biosynthesis From L-Serine of Processed Iranian Beet and Cane Molasses and Indole by Induced Escherichia coli ATCC 11303 Cells

Sadeghiyan-Rizi

Fooladi

Heravi

et al. 2014

Jundishapur J Microbiol

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Background:L-tryptophan is an important ingredient in medicines, especially in neuromedicines such as antidepressants. Many commercial processes employ various microorganisms with high tryptophan synthase activity to produce L-tryptophan from indole and L-serine, but these processes are very costly due to the costs of precursors, especially L-serine.Objectives:For this reason, we studied the ability to use processed Iranian cane and beet molasses as L-serine sources for L-tryptophan production, which enables us to reach a cost-effective process.Materials and Methods:Whole cells of Escherichia coli ATCC 11303 were induced for L-tryptophan synthase by addition of indole to the growth medium and bacterial cells harvested from the growth medium were used as biocatalysts in the production medium. Conditions of the production medium were optimized and Iranian cane and beet molasses were processed by solvent extraction with ethanol and n-butanol and used as L-serine sources of the production medium. Amount of L-tryptophan and theoretical yield of L-tryptophan production were determined by High Performance Liquid Chromatography and by a colorimetrical method on the basis of the remaining indole assay, respectively.Results:L-tryptophan production increased by 15 folds, when indole was used as an inducer. L-tryptophan was produced from processed Iranian beet molasses in satisfactory amounts (0.53 mM) and no exogenous pyridoxal phosphate was required as a cofactor under our experimental conditions.Conclusions:The obtained results proved that Iranian beet molasses include significant amounts of L-serine that makes them a suitable substitution for L-serine. Findings of the present study give impetus to use of Iranian beet molasses for cost-effective L-Trp production in the amino acid industry.

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Section: Discussionmentioning

confidence: 99%

Optimization of L-Tryptophan Biosynthesis From L-Serine of Processed Iranian Beet and Cane Molasses and Indole by Induced Escherichia coli ATCC 11303 Cells

Sadeghiyan-Rizi

Fooladi

Heravi

et al. 2014

Jundishapur J Microbiol

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“…However, there has been controversy about which of the two has better properties for immobilization of microorganisms. Some researchers pointed out that carrageenan immobilized beads offered optimal conditions (Langrene et al, 1984;Tosa et al, 1979), whereas others found alginate had better stability (Marwaha and Kennedy, 1984;Nilsson et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Alginate and Carrageenan Immobilization Effects on Schizosaccharomyces Pombe Activity and Stability

Bonilla

Rand

1991

Journal of Food Science

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Production of L-DOPA from pyrocatechol and DL-serine by bioconversion using immobilized Erwinia herbicola cells

1984

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Entrapment of l-tryptophan producing Escherichia coli in different matrices: activity of immobilized cells

Cited by 7 publications

References 11 publications

Optimization of L-Tryptophan Biosynthesis From L-Serine of Processed Iranian Beet and Cane Molasses and Indole by Induced Escherichia coli ATCC 11303 Cells

Optimization of L-Tryptophan Biosynthesis From L-Serine of Processed Iranian Beet and Cane Molasses and Indole by Induced Escherichia coli ATCC 11303 Cells

Alginate and Carrageenan Immobilization Effects on Schizosaccharomyces Pombe Activity and Stability

Production of L-DOPA from pyrocatechol and DL-serine by bioconversion using immobilized Erwinia herbicola cells

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