Production of L-DOPA from pyrocatechol and DL-serine by bioconversion using immobilized Erwinia herbicola cells

Formation of melanin pigment by a mutant of Bacillus thuringiensis H-14

“…for 10 min, followed by S. L. Hoti and K. Balaraman filtration through a 045 pm pore-size membrane filter. The filtrate was tested for the production of ~-3 , 4 dihydroxyphenylalanine (L-DOPA) using the chemical method of Arnow (1937), high-performance liquid chromatography (HPLC) (Para et al, 1984), and mass spectroscopy (MS) (Huizing et al, 1985).…”

High performance microbiological transformation of L-tyrosine to L-dopa by Yarrowia lipolytica NRRL-143

“…The process of bioconversion of L-tyrosine to L-dopa in microorganisms is generally slow, but is accelerated by a small amount of L-dopa in the broth [ 7 ]. L-dopa has also been produced with Erwinia herbicola cells carrying a mutant transcriptional regulator TyrR from pyrocatechol and DL-serine [ 8 , 9 ]. It can also be produced using L-tyrosine as a substrate, tyrosinase as a biocatalyst and L-ascorbate as the reducing agent [ 10 , 11 ].…”

Biocatalysis with Immobilized Cells

“…Immobilized cells of Erwinia herbicola were used for the production of Ldopa from pyrocatechol and DL-serine (Para, Rifai and Baratti, 1984). The optimum conditions for L-dopa synthesis were 37 C, 130 mm ammonium acetate, 100 mm DL-serine and 64 Mt pyrocatechol.…”