Enhancing the Electrochemiluminescence of Luminol by Chemically Modifying the Reaction Microenvironment

Qiao, Yali; Li, Yuan; Fu, Wen; Guo, Zhihui; Zheng, Xusheng

doi:10.1021/acs.analchem.8b02577

Cited by 48 publications

(31 citation statements)

References 41 publications

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“…More importantly, the performance comparison between the obtained biosensor and the previous reports illustrated that the biosensor possessed a higher sensitivity and lower LOD, which testified the potential of this biosensor in bioassay (Table ). − …”

Section: Resultsmentioning

confidence: 99%

Target-Induced 3D DNA Network Structure as a Novel Signal Amplifier for Ultrasensitive Electrochemiluminescence Detection of MicroRNAs

et al. 2019

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Here, a target-induced three-dimensional DNA network structure (T-3D Net) produced by catalytic hairpin assembly (CHA) was proposed as a novel signal amplifier to fabricate an ultrasensitive electrochemiluminescence (ECL) biosensor for microRNAs detection. Usually, conventional CHA can produce only one output DNA in each target cycle, while the proposed strategy could produce multiple output DNA by using DNA-functionalized magnetic beads (MBs) and gold nanoparticles (AuNPs) to form T-3D Net. Then, the T-3D Net with high loading capacity could be completely collapsed by dissolving AuNPs to efficiently convert trace microRNA-21 into a large amount of output DNA. Furthermore, the nanocomposite containing Ru(bpy)3 2+ as luminophore and boron nitride quantum dots (BNQDs) as coreactant provided a strong initial ECL response owing to the short electron transfer distance between luminophore and coreactant (signal-on). Next, the DNA duplex probes labeled with N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and dopamine (DA) (S1-ABEI/S2-DA) were further immobilized on the nanocomposite to reduce the background signal due to the double quenching effect of DA for both ABEI and Ru(bpy)3 2+ (signal-off). In the presence of the output DNA with enzyme-assisted self-recycling, S2-DA was displaced and detached from the electrode surface to achieve ECL signal recovery. Simultaneously, S1-ABEI restored a stable hairpin structure, making ABEI close to the electrode surface for more effective resonance energy transfer (RET) between ABEI and Ru(bpy)3 2+, which greatly improved the final ECL response (signal-super on). Thus, the ECL biosensor demonstrated superior performance for ultrasensitive detection of microRNA-21 with low detection limit (0.33 aM) and was successfully applied to monitor the expression of microRNA-21 in human cancer cell lysates. This strategy provided an ultrasensitive way for the detection of biomolecules and revealed an effective avenue for diseases diagnosis.

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Section: Resultsmentioning

confidence: 99%

Target-Induced 3D DNA Network Structure as a Novel Signal Amplifier for Ultrasensitive Electrochemiluminescence Detection of MicroRNAs

et al. 2019

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“…Electrochemiluminescence (ECL), presently, is widely reported in clinical laboratory diagnosis, , food safety supervision, environmental pollution monitoring, cell imaging, and the biosensing field due to the high sensitivity, facile controllability, and simplified optical equipment. , To further boost the sensitivity of an ECL sensor, nanomaterials , as a signal amplification strategy played an increasingly important role for their excellent functions, for instance, luminophore load, biomolecules immobilization, interface modification, and enzyme simulation, which have been extensively applied to construct ECL biosensors. Since Bard’s group reported the first ECL study on quantum dots (QDs), miscellaneous nanomaterials were extensively applied as ECL emitters. − Although these nanomaterials have the advantages of excellent immobilization capacity, the dense nanomaterials caused a serious inner filter effect to reduce the ECL efficiency.…”

mentioning

confidence: 99%

Three-Dimensional Cadmium Telluride Quantum Dots–DNA Nanoreticulation as a Highly Efficient Electrochemiluminescent Emitter for Ultrasensitive Detection of MicroRNA from Cancer Cells

et al. 2019

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In this work, a novel three-dimensional cadmium telluride quantum dots–DNA nanoreticulation (3D CdTe QDs–DNA-NR) was used as a signal probe with the dual-legged DNA walker circular amplification as target conversion strategy to establish a pioneering electrochemiluminescence (ECL) biosensing strategy for ultrasensitive detection of microRNA-21 form cancer cells. Herein, such a 3D luminous nanomaterial with reticular structure not only supported abundant CdTe QDs to avoid the inner filter effect for obtaining a high ECL efficiency but also contained the hemin/G-quadruplex as coreaction accelerator in the 3D CdTe QDs–DNA-NR/S2O8 2– system for the enhancement of ECL intensity. Furthermore, with the target-induced dual-legged DNA walker circular amplification strategy, a mass of output DNA was produced to connect with the 3D CdTe QDs–DNA-NR for the construction of the ECL biosensor, which realized the ultrasensitive detection of microRNA-21 from 100 aM to 100 pM and the detection limit down to 34 aM. Significantly, this work could be readily extended for the detection of other biomolecules to provide a neoteric channel for disease diagnosis.

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“…0.50 V under cyclic voltammetry in PBS with H2O2 as the co-reagent. Such oxidation could be assigned to the electron loss of the LU donor, 59,60 as it was not observed for the single dyes (CO and NA) in the same condition (Figure S16). Simultaneously, LU-CO and LU-NA exhibited ECL signals from the starting of the electrochemical oxidation, and reached the maximum intensity at ca.…”

Section: Resultsmentioning

confidence: 72%

Lighting up Electrochemiluminescent Inactive Dyes by Intramolecular Resonance Energy Transfer

Zheng¹,

Yang²,

Zhao³

et al. 2021

Preprint

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By virtue of near-zero optical background and photobleaching, electrochemiluminescence (ECL), an optical phenomenon excited by electrochemical reactions, has drawn extensive attention in both fundamental studies and wide applications especially of ultrasensitive bioassay. Developing diverse ECL emitters is crucial to unlock their multiformity and performances, but remains a formidable challenge, due to the rigorous requirements for ECL. Herein, we report a general intramolecular ECL resonance energy transfer (iECL-RET) strategy to light up ECL-inactive dyes in aqueous solutions using an existing high-performance ECL initiators. As a proof-of-concept, a series of luminol donor-dye acceptor based ECL emitters with near unity RET efficiency and coarse/fine tunable emission wavelengths were demonstrated. Different to previous exploitation of new molecule single-handedly to address all the prerequisites of ECL, each unit in the proposed ECL ensemble performed maximally its own functions. The iECL-RET strategy would greatly expand the family members of ECL emitters for more demanding future applications.

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Enhancing the Electrochemiluminescence of Luminol by Chemically Modifying the Reaction Microenvironment

Cited by 48 publications

References 41 publications

Target-Induced 3D DNA Network Structure as a Novel Signal Amplifier for Ultrasensitive Electrochemiluminescence Detection of MicroRNAs

Target-Induced 3D DNA Network Structure as a Novel Signal Amplifier for Ultrasensitive Electrochemiluminescence Detection of MicroRNAs

Three-Dimensional Cadmium Telluride Quantum Dots–DNA Nanoreticulation as a Highly Efficient Electrochemiluminescent Emitter for Ultrasensitive Detection of MicroRNA from Cancer Cells

Lighting up Electrochemiluminescent Inactive Dyes by Intramolecular Resonance Energy Transfer

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