Enhancing stabilities of lipase by enzyme aggregate coating immobilized onto ionic liquid modified mesoporous materials

Zou, Bin; Song, Chunyan; Xu, Xiaping; Xia, Jiaojiao; Huo, Shuhao; Cui, Fengjie

doi:10.1016/j.apsusc.2014.04.210

Cited by 49 publications

(33 citation statements)

References 32 publications

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“…Efficient immobilization is the result of the perfect matching of several factors depending on the enzyme, the process, the immobilization support, and the additives used for modification supports [ 11 , 12 , 13 , 14 ]. Therefore, the use of an appropriate support can influence the biocatalyst physical-chemical properties, such as diffusion and catalytic efficiency on each specific reaction system [ 15 , 16 ]. Thus, tailored solutions must be developed for each specific process of interest.…”

Section: Introductionmentioning

confidence: 99%

Lipase Immobilization on Silica Xerogel Treated with Protic Ionic Liquid and its Application in Biodiesel Production from Different Oils

Carvalho

Vidal

Barbosa

et al. 2018

IJMS

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Treated silica xerogel with protic ionic liquid (PIL) and bifunctional agents (glutaraldehyde and epichlorohydrin) is a novel support strategy used in the effective immobilization of lipase from Burkholderia cepacia (LBC) by covalent binding. As biocatalysts with the highest activity recovery yields, LBC immobilized by covalent binding with epichlorohydrin without (203%) and with PIL (250%), was assessed by the following the hydrolysis reaction of olive oil and characterized biochemically (Michaelis–Menten constant, optimum pH and temperature, and operational stability). Further, the potential transesterification activity for three substrates: sunflower, soybean, and colza oils, was also determined, achieving a conversion of ethyl esters between 70 and 98%. The supports and the immobilized lipase systems were characterized using Fourier transform infrared spectra (FTIR), scanning electron microscopy (SEM), elemental analysis, and thermogravimetric (TG) analysis.

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Section: Introductionmentioning

confidence: 99%

Lipase Immobilization on Silica Xerogel Treated with Protic Ionic Liquid and its Application in Biodiesel Production from Different Oils

Carvalho

Vidal

Barbosa

et al. 2018

IJMS

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“…An enzymatic reaction is affected by temperature and many studies showed that the optimum activity of the enzyme occurs at a temperature between 35 -40 °C [22,32]. In the case of lipase, the optimal temperature was reported at 37-40 °C [33].…”

Section: Temperature Effect On Pyridinium Ligands In the Absence And mentioning

confidence: 99%

“…Commercial lipases could also be used in biocatalytic reactions to obtain indolizines [9]. Due to their eco-friendly and recyclable properties, lipases are involved in the synthesis of tricyanovinylated compounds [21] and in the design of mesoporous materials [22], green polymers [23] and bioelectrodes used to detect triglycerides in human serum [24]. There are many methods, both analytical and electrochemical, to detect lipase activity.…”

Section: Introductionmentioning

confidence: 99%

The interactions between lipase and pyridinium ligands investigated by electrochemical and spectrophotometric methods

Patriche

Lupu

Cârâc

et al. 2016

J. Electrochem. Sci. Eng.

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The interaction between pyridinium ligands derived from 4,4’-bipyridine (N,N’-bis(p-bromophenacyl)-4,4’-bipyridinium dibromide – Lr) and (N,N’-bis(p-bromophenacyl)-1,2-bis (4-pyridyl) ethane dibromide – Lm) with lipase enzyme was evaluated. The stability of the pyridinium ligands, having an essential role in biological systems, in 0.1 M KNO3 as supporting electrolyte is influenced by the lipase concentration added. The pH and conductometry measurements in aqueous solution suggest a rapid ionic exchange process. The behavior of pyridinium ligands in the presence of lipase is investigated by cyclic voltammetry and UV/Vis spectroscopy, which indicated bindings and changes from the interaction between them. The voltammograms recorded on the glassy carbon electrode showed a more intense electronic transfer for the Lr interaction with lipase compared to Lm, which is due to the absence of mobile ethylene groups from Lr structure.

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“…12,13 Other advantages is the fact that silica is insoluble in aqueous media, thermally, mechanically and chemically stable, in addition to resistance to microbial attacks. 14 In addition, silica have functional groups on its surface that facilitate the enzyme binding and also the possibility of surface modification, such as pore enlargement and volume with the use of modifying agents, 15 which in this work is used the protic ionic liquid for this modification.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Performance of a Packed Bed Reactor to Production Ethyl Esters From Crude Vegetable Oil Using Lipase Immobilized in Silica Modified With Protic Ionic Liquid

Santana¹,

Oliveira²,

Carvalho³

et al. 2018

Quim. Nova

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The objective this study was to evaluated the influence of silica modified with protic ionic liquid (PIL) on an immobilized biocatalyst by covalent binding of Burkholderia cepacia lipase (BCL) in a PBR and in the transesterification reaction, by characterization and mass transfer of the system studied. The results showed that maximum conversion of ethyl esters was 39 ± 1.73% at 96 h (IB on control silica) and 47 ± 2.0% in 72 h (IB on modified silica). The PIL also influenced the analysis of residence time distribution (RTD) with the mean time ranging from 14.94 (control silica) to 25 min (modified silica). Variation of the reaction parameters (temperature, flow rate and molar ratio) positively influenced the mass transfer coefficient (kc) which varied from 0.95 × 10 −4 to 2.39 × 10 −4 m s −1 (IB on control silica) and from 1.22 × 10 −4 to 3.06 × 10 −4 m s −1 (IB on modified silica).

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Enhancing stabilities of lipase by enzyme aggregate coating immobilized onto ionic liquid modified mesoporous materials

Cited by 49 publications

References 32 publications

Lipase Immobilization on Silica Xerogel Treated with Protic Ionic Liquid and its Application in Biodiesel Production from Different Oils

Lipase Immobilization on Silica Xerogel Treated with Protic Ionic Liquid and its Application in Biodiesel Production from Different Oils

The interactions between lipase and pyridinium ligands investigated by electrochemical and spectrophotometric methods

Analysis of the Performance of a Packed Bed Reactor to Production Ethyl Esters From Crude Vegetable Oil Using Lipase Immobilized in Silica Modified With Protic Ionic Liquid

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