Lipase Immobilization on Silica Xerogel Treated with Protic Ionic Liquid and its Application in Biodiesel Production from Different Oils

Abstract

Treated silica xerogel with protic ionic liquid (PIL) and bifunctional agents (glutaraldehyde and epichlorohydrin) is a novel support strategy used in the effective immobilization of lipase from Burkholderia cepacia (LBC) by covalent binding. As biocatalysts with the highest activity recovery yields, LBC immobilized by covalent binding with epichlorohydrin without (203%) and with PIL (250%), was assessed by the following the hydrolysis reaction of olive oil and characterized biochemically (Michaelis–Menten constant, optimum pH and temperature, and operational stability). Further, the potential transesterification activity for three substrates: sunflower, soybean, and colza oils, was also determined, achieving a conversion of ethyl esters between 70 and 98%. The supports and the immobilized lipase systems were characterized using Fourier transform infrared spectra (FTIR), scanning electron microscopy (SEM), elemental analysis, and thermogravimetric (TG) analysis.

Proteins in Ionic Liquids: Reactions, Applications, and Futures

“…Lipase biotransformations have significant application, and this is reflected in the ionic liquid field by a substantial literature (Itoh et al, 2002; Zhao et al, 2010a, 2018; Latif et al, 2014; Kim et al, 2016; Carvalho et al, 2018; Itoh, 2018; Lisboa et al, 2018; Park et al, 2018; Zhong et al, 2018). As such, lipase-catalyzed biotransformations have been the topic of recent reviews, covering enantioselective transesterification, biofuel production and polymer synthesis in ionic liquids (Itoh, 2017a; Elgharbawy et al, 2018), and ionic liquid-mediated activation of lipase-catalyzed reactions (Itoh, 2018).…”

“…This challenge may soon be overcome, with an effective model reaction to describe the inactivation of the lipase Novozyme 435 recently being proposed, and a circulating feed mixture being introduced into the reactor design to remove the by-product glycerine (Endo et al, 2018). The immobilization of Burkholderia cepacia lipase onto silica xerogel with protic ionic liquid ( N -methylmonoethanolamine pentanoate) and in-depth characterization of the activity and operational stability could afford appropriate catalysts for such a circulating reactor (Carvalho et al, 2018). Experimental data of the physical adsorption of B. cepacia lipase on an aerogel-ionic liquid system based on [N 1, 1, 1, 18 ][NTf 2 ], with an isotherm model fitted to the data, provides an alternative, characterized immobilization system (Lisboa et al, 2018).…”

Immobilization of lipase in silica gel from rice husk ash and its activity assay to hydrolyze palm oil

“…Immobilized lipase in silica gel had stronger interaction between siloxane and silanol group than silica gel blank, so the peak was sharper since the immobilized one had reacted with lipase. Compared to silica gel blank, there was no indication of C=N and C=O (1797.66 cm -1 ) which are the protein groups [15].…”

Facilitating enzymatic reactions by using ionic liquids: A mini review

“…Lipases are common components of ILe enzyme systems. Such reactions include surfactant synthesis [23], food and medicinal applications [24], ester synthesis [25], biodiesel preparation [26], Network visualization of keywords from the Scopus database (2018-2020) for the search terms 'ionic liquids' and 'enzyme,' as of July 20, 2020.…”