Analysis of the Performance of a Packed Bed Reactor to Production Ethyl Esters From Crude Vegetable Oil Using Lipase Immobilized in Silica Modified With Protic Ionic Liquid

Abstract

The objective this study was to evaluated the influence of silica modified with protic ionic liquid (PIL) on an immobilized biocatalyst by covalent binding of Burkholderia cepacia lipase (BCL) in a PBR and in the transesterification reaction, by characterization and mass transfer of the system studied. The results showed that maximum conversion of ethyl esters was 39 ± 1.73% at 96 h (IB on control silica) and 47 ± 2.0% in 72 h (IB on modified silica). The PIL also influenced the analysis of residence time distribution (RTD) with the mean time ranging from 14.94 (control silica) to 25 min (modified silica). Variation of the reaction parameters (temperature, flow rate and molar ratio) positively influenced the mass transfer coefficient (kc) which varied from 0.95 × 10 −4 to 2.39 × 10 −4 m s −1 (IB on control silica) and from 1.22 × 10 −4 to 3.06 × 10 −4 m s −1 (IB on modified silica).

Repeated batches as a strategy for high 2G ethanol production from undetoxified hemicellulose hydrolysate using immobilized cells of recombinant Saccharomyces cerevisiae in a fixed-bed reactor

“…Furthermore, the use of a fixed-bed reactor can also contribute to an economically feasible process because of its specific advantages, such as high conversion rate per weight of biocatalyst, high productivity and reduced cost of operation and maintenance, due to the possibility of long operating periods and easy biocatalyst recovery [20,21]. The fixed-bed bioreactor allows process operation in repeated batches, which is an interesting approach for bioethanol production using immobilized yeast cells since the microorganism can be immediately reutilized in a new fermentation batch, with no need of a centrifugation step, reducing process time and cost and consequently improving productivity [22].…”

Continuous Enzymatic Synthesis of 2-Ethylhexyl Oleate in a Fluidized Bed Reactor: Operating Conditions, Hydrodynamics, and Mathematical Modeling

“…The k cs values shown in Table 5 were calculated from the literature. 23 To reduce the number of parameters of the model, we considered that the k cs of oleic acid was equal to that of alcohol, ester, and water for each bed porosity condition evaluated.…”

“…However, with increasingly high recycle flow rates (such as that used in the present study, 1.4 mL min –1 ), the difference increases, promoting reagent dilution and consequently affecting mass transfer rates. The k cs values shown in Table were calculated from the literature . To reduce the number of parameters of the model, we considered that the k cs of oleic acid was equal to that of alcohol, ester, and water for each bed porosity condition evaluated.…”

“…20−22 To optimize the performance of a bioreactor, it is necessary to understand its operational behaviors, hydrodynamic characteristics, and mass transfer parameters. 23 Mathematical modeling is an important tool to describe and predict the behavior of a bioprocess. This study aimed to analyze the operational parameters, characterize the hydrodynamic behavior, and model the synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase (CALB) immobilized on magnetic poly(styrene-co-divinylbenzene) (STY-DVB-M) in a continuous fluidized bed bioreactor.…”

“…To optimize the performance of a bioreactor, it is necessary to understand its operational behaviors, hydrodynamic characteristics, and mass transfer parameters . Mathematical modeling is an important tool to describe and predict the behavior of a bioprocess.…”