Enhancing Copper and Lead Bioaccumulation in Rapeseed by Adding Hemp Shives as Soil Natural Amendments

Abstract

The current study reveals the results of a phytoremediation process applied to a multi metal contaminated soil, located in close proximity to an energy power plant. Phytoremediation process was studied using the rapeseed cultivation, both in the presence and absence of hemp shives, considered as potential natural soil amendments. The physiological responses of the rapeseed plants, such as variations in length and accumulation of biomass, as well as the content of assimilating pigments were investigated. The concentrations of Cu (II) and Pb (II) ions have also been determined in soil and plants aiming at locating the metal ions in different organs of the plants. By using hemp shives as amendments, bioaccumulation of copper and lead ions in plant increases. The plants cultivated in the presence of high amounts of heavy metal ions have been characterized by an inhibition of growth and development that consisted in decrease of root and stem lengths, as well in the amount of biomass accumulated in different vegetative organs.

Histo-anatomic Aspects on Zea Mays L. Influenced by Hemp Shives Polyphenolic Extract

“…The characterization of hemp shives extract was made in a past work (Tanase et al, 2014b). Thus, the total polyphenols content of hemp shives aqueous extract was 164 mg GAE/100g.…”

“…For the HPLC analysis, the cumulated aqueous extracts were concentrated under vacuum to 10 mL. These results as well as the extraction method were published in our previous work (Tanase et al, 2014b).…”

“…At low concentrations in soil the presence of hemp shives stimulates lead and copper bioaccumulation, promoting the translocation of heavy metals to the aerial part of the plant. In this case the polyphenolic compounds from hemp shives increase the solubilisation of heavy metals and improve the phytoextraction process (Tanase et al, 2014b). The present research is concerned with the use of the wastes from the industrial hemp, Cannabis sativa L., to obtain polyphenolic extract and to identify the internal structure changes in maize (Zea mays L.) seedlings, under the influence of hemp shives polyphenolic extract.…”

Determination of Traces Heavy Metals from Solid Residues Formed by Combustion of Lignite in Relation to Degree of Accessibility in Soil and Plants

“…Thus, the lead concentration in soil in the ash deposit area (70.20 mg Kg -1 ) exceeds the set threshold for susceptible soils of 50 mg.kg -1 . The transfer capacity of heavy metals from soil to plants was generally described using translocation factor (TF), calculated as the ratio of the metal concentration in the plants and the concentration of the same metal in the plant's own soil [27][28][29]. TF values around 0.1 do not indicate bioaccumulation of metals; values around 0.5 indicate an accumulation that should be considered for food safety (value of reference in this study).…”

New Aspects of Biomass Waste Valorization: Spruce Bark Crude Extracts as Plant Growth Regulators

“…Natural polyphenols' utilization in the plant science world is an innovative concept, which shows increasing success. The effects of polyphenolic extracts as antioxidant agents, plant growth regulators, or bioremediation agents have been successfully tested on a large group of plants, such as soybean, sunflower, bean, oat, rape, maize, and tomatoes (Bălaş and Popa 2007;Stingu et al 2009a;Tanase et al 2014;Bujor et al 2015;Talmaciu et al 2015). Also, prior research details the influence of phenolic compounds in seed germination and plant development processes, either as individual compounds or as global extracts recovered from different bioresources (Almaghrabi 2012).…”