Enhancement of the oligosaccharide synthetic activity of β-galactosidase in organic solvents by cyclodextrin

Transgalactosylation of lactose for synthesis of galacto-oligosaccharides using Kluyveromyces marxianus NCIM 3551

“…5a). This compared well to the GOS yield of 37% (w/w of substrate) obtained at low substrate concentration (10.8%, w/v) using b-galactosidase from A. oryzae in aqueousorganic co-solvent mixture [22]. A yield of 25.1% (w/w) was attained at 10% lactose concentration using K. marxianus var.…”

The enzymatic production of lactulose via transglycosylation in conventional and non-conventional media

“…Furthermore, it does not have a functional group, such as OH, that might interact with the active site of the enzyme and lead to the production of unwanted by-products . TEP was chosen because it has all the advantages mentioned above for acetone, but, in addition, is less hydrophilic (less polar) (Finich & Yoon, 1997;Hua et al, 2010;Srisimarat & Pongsawasdi, 2008).…”

“…Maugard, Gaunt, Legoy, and Besson (2003) found that the use of hexanolebuffer system (70:30, v/v) as the reaction media for microwave-assisted synthesis of galactooligosaccharides (GOS) catalysed by immobilised lactase results in an increase of 217-fold in the selectivity of the reaction. Srisimarat and Pongsawasdi (2008) observed a 1.6-fold increase in product yield of catalysed synthesis of oligosaccharide when buffer media was replaced with aqueouseorganic co-solvent mixtures (TEP). Isabel and Otero (2003) found a 33% increase in the yield of 6-galactosyl lactose when polyethylene glycol as solvent instead of conventional aqueous solutions.…”

“…The term non-conventional media in biocatalysis alludes to systems that take advantage of solvents other than water, or use the addition of components to aqueous systems in order to favour particular characteristics of the biocatalyst or the reaction catalysed by it (Krieger et al, 2004). The advantages of performing enzyme catalysed biochemical reactions in organic solvents include: (i) increased solubility of non-polar substrates and products, which markedly speeds up overall reaction rates (Srisimarat & Pongsawasdi, 2008;Torres & Castro, 2004); (ii) reversal of thermodynamic equilibrium in favour of synthesis over hydrolysis, allowing reactions usually not favoured in aqueous solutions to occur (e.g., transesterification, thioesterification, aminolysis; Cruz- Guerrero, Gómez-Ruiz, Viniegra-González, Bárzana, & García Garibay, 2006;Hua et al, 2010;Srisimarat & Pongsawasdi, 2008;Torres & Castro, 2004;Yoon & Mckenzie, 2005); (iii) changes the enantioselectivity of the enzyme (Klibanov, 2001;Torres & Castro, 2004;Watanabe & Ueji, 2001). Maugard, Gaunt, Legoy, and Besson (2003) found that the use of hexanolebuffer system (70:30, v/v) as the reaction media for microwave-assisted synthesis of galactooligosaccharides (GOS) catalysed by immobilised lactase results in an increase of 217-fold in the selectivity of the reaction.…”

Spectrofluorometry study of β-cyclodextrin and N-phenyl-1-naphthylamine inclusion complex and its analytical application via artificial neural network

“…It is due to the well-known properties of bCD in forming inclusion complexes with various guest molecules of different polarities and physical dimensions [14][15][16][17]. Based on this, bCD has been readily adopted in various applications such as for developing analytical methods [18,19], enzyme-subtract interactions [20], drug deliveries [21] and catalytic activities [22]. In addition, the formation of inclusion complex can alter the photochemical and photo-physical properties of the guest molecules, which turns to be useful for quantification purposes.…”