Spectrofluorometry study of β-cyclodextrin and N-phenyl-1-naphthylamine inclusion complex and its analytical application via artificial neural network

Abstract

The interaction between b-cyclodextrin (bCD) and N-phenyl-1-naphthylamine has been studied spectrofluorimetrically and found to form a 1:1 (bCD:NPN) inclusion complex at optimum conditions of pH 8 with the formation time of 120 s. The association constant of the complex was established to be 6.0 9 10 2 M, while a remarkable enhancement in fluorescence intensity was also observed at 445 nm with excitation of 334 nm. A spectroflurometric method for the detection of N-phenyl-1-naphthylamine has been developed having a dynamic range linear up to 4.67 9 10 -7 M with a limit of detection of 0.58 nM. The repeatability study at two different bCD concentrations of 1.0 9 10 -4 and 4.0 9 10 -4 M was found to give RSD values of 2.40 and 1.42%, respectively. Artificial neural network (ANN) has been utilised to model the analytical system and successfully extended the analytical dynamic range up to 8.0 9 10 -7 M from the original 4.67 9 10 -7 M, brief network training and the optimum parameters of are described in this work.

Spectrofluorometric analytical applications of cyclodextrins

“…Recently Ng and Narayanaswamy investigated the host–guest interaction between β‐CD and N‐phenyl‐1‐naphthylamine . Based on the enhancement in fluorescence intensity observed at 445 nm upon excitation at 334 nm, a spectrofluorometric method for determination of N‐phenyl‐1‐naphthylamine was proposed.…”