Enhancement of resistance in mice treated with Lactobacillus casei: Effect on Trichinella spiralis infection

Garfias, Carlos Ramón Bautista; Ixta, O; Orduña, M; Martínez, F.; Aguilar, Blanca R.; Cortés, Alba

doi:10.1016/s0304-4017(98)00210-6

Cited by 41 publications

(26 citation statements)

References 21 publications

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“…Stefanski and Przyjalkowski showed that monoassociation of mice with various rat lactobacilli was not favorable to the establishment of T. spiralis in the intestines compared to those of control conventional animals (29). Moreover, in a recent study, intraperitoneal administration of L. casei has been demonstrated to enhance protective immunity to T. spiralis in NIH mice (2). In our studies, in which the lactobacilli and bifidobacteria were administered orally instead of intraperitoneally, as described in the study by Bautista-Garfias et al, no definite effects on pathology could be found, despite the enhanced DTH and IgG2b responses directed against T. spiralis antigen.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Antigen-Specific Delayed-Type Hypersensitivity and Immunoglobulin G2b Responses after Oral Administration of ViableLactobacillus caseiYIT9029 in Wistar and Brown Norway Rats

Waard¹,

Garssen²,

Snel³

et al. 2001

Clin Diagn Lab Immunol

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In this study, the effects of orally administered viable Lactobacillus casei Shirota strain YIT9029 on the immunity parameters of Wistar and Brown Norway rats were examined. For this purpose, we used the Trichinella spiralis host resistance model. Two weeks before and during T. spiralis infection, rats were fed 10 9 viable L. casei bacteria 5 days per week. The T. spiralis-specific delayed-type hypersensitivity (DTH) response was significantly enhanced in both Wistar and Brown Norway rats given L. casei. In both rat strains fed L. casei, serum T. spiralis-specific immunoglobulin G2b (IgG2b) concentrations were also significantly increased. In the model, no significant effects of L. casei on larval counts or inflammatory reactions in the tongue musculature, body weights, or lymphoid organ weights were observed. Serum specific antibody responses, other than IgG2b, were not changed by feeding of L. casei. In contrast to L. casei, it was shown that orally administered Bifidobacterium breve or Bifidobacterium bifidum had no influence on the measured infection and immunity indices in the rat infection model. Since the rat DTH response is considered to be a manifestation of Th1 cell-mediated immunity and the IgG2b isotype has been associated with Th1 activity, it was concluded that Th1 cells could play an active role in the immunomodulatory effects of orally administered L. casei. Furthermore, our data do not indicate that the effect of oral supplementation with L. casei is dependent on the genetic background of the host.

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Section: Discussionmentioning

confidence: 99%

Enhanced Antigen-Specific Delayed-Type Hypersensitivity and Immunoglobulin G2b Responses after Oral Administration of ViableLactobacillus caseiYIT9029 in Wistar and Brown Norway Rats

Waard¹,

Garssen²,

Snel³

et al. 2001

Clin Diagn Lab Immunol

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“…Percent parasitemia and percentage of CF were recorded from days 0 to 31 post-infection. This treatment was chosen on the basis of results of previous studies in which protection against T. spiralis was observed when L. casei was administered 7 days before infection (Bautista-Garfias et al 1999.…”

Section: Experimental Designmentioning

confidence: 99%

“…Isospora felis infections, BCG from Mycobacterium bovis or M. phlei, and killed Corynebacterium parvum can also protect mice against babesial infections (Clark et al 1976Corrier and Wagner 1984;Takahashi et al 1993;Tewari et al 1996). On the other hand, it has been demonstrated that the immunostimulant non-pathogenic bacteria Lactobacillus casei, when inoculated 1 week before infection, can induce a protective response against infections with parasites such as Trichinella spiralis in mice (Bautista-Garfias et al 1999 and Eimeria spp. in broiler chickens (BautistaGarfias et al 2003).…”

Section: Introductionmentioning

confidence: 97%

The treatment of mice with Lactobacillus casei induces protection against Babesia microti infection

Garfias

Gómez²,

Aguilar³

et al. 2005

Parasitol Res

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In this study, we report that administration of Lactobacillus casei confers protection to mice against the intracellular protozoan Babesia microti. Mice treated with L. casei orally or intraperitoneally were inoculated 7 days later with an infectious dose of B. microti. Mice treated with lactobacilli showed significant reduction in the percentage of parasitized erythrocytes (PPE) compared to untreated mice. When mice were inoculated intraperitoneally with L. casei 3 or 0 days before challenge with B. microti, the PPE was significantly lower compared to untreated mice and there were no differences between treated mice and mice immune to B. microti infection. When mice treated with live or dead L. casei were compared to mice inoculated with Freund Complete Adjuvant before a B. microti infection, a significant reduction of PPE was observed. These results show the protective effect of L. casei administered to mice against a B. microti infection and suggest that it might act by stimulating the innate immune system.

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“…A reduced shortening of the intestinal villi during trichinellosis was also observed in mice infected with Lactobacillus casei, which produce lactic acid (Bautista-Garfias et al, 1999). In the study of Petkevicius et al (2003) cited above, the lower number of parasites in the large intestines in pigs after administration of highly fermentable dietary carbohydrates was accompanied by changes in the structure of intestinal mucosa.…”

Section: Resultsmentioning

confidence: 88%

The influence of orally administered short chain fatty acids on intestinal histopathological changes and intensity of Trichinella spiralis infection in mice

Miśta¹,

Piekarska²,

Houszka³

et al. 2010

Vet. Med. - Czech

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ABSTRACT:The influence of short chain fatty acids (SCFA) on histopathological changes in the small intestine and the intensity of invasion of T. spiralis in mice were investigated in this study. The animals were infected with doses of 500 and 250 T. spiralis larvae per mouse. A SCFA solution containing acetic, propionic and butyric acid (30 : 15 : 20mM) was administered orally to the mice starting from the 5 th day before infection to the 20 th day after infection (day). Fragments of the jejunum collected during dissection on the 7 th and 10 th day were used to prepare specimens to assess the histopathological changes. In the infected animals, the intestinal trichinellae were counted on the 7 th and 10 th day, while on the 42 nd day the muscle larvae number were determined. The strongest host reaction in the intestine was observed on the 7 th day at a dose of T. spiralis 500 larvae, and on the 10 th day at a dose of 250 larvae. Numerous inflammatory infiltrations, strong shortening of the intestinal villi, extension of the intestinal crypts, and the lowest ratio of the villi length to the intestinal crypts depth were observed. The ratio was 1.3 ± 0.3 on the 7 th day at a dose of 500 larvae, and on the 10 th day, at dose of 250 larvae the ratio reached 1.5 ± 0.5. Both values differed significantly from the control group: 3.3 ± 0.5 (P < 0.01). Administration of SCFA to the animals infected with T. spiralis caused remission of local histopathological changes resulting from the presence of the parasite in the small intestine after the mentioned periods. This manifested as limited villi shortening and reduced deepening of intestinal crypts. At the higher infectious dose, in animals receiving the acid solution, on the 7 th day the intestinal villi were considerably longer (356 µm ± 35) than in the group infected with T. spiralis but not treated with the acids (279 µm ± 57; P < 0.01). At a lower dose of parasites, on the 10 th day these values were 339 µm ± 88 and 306 µm ± 47 respectively and the observed differences were not statistically significant. The solution of SCFA also caused a decrease in the numbers of mature parasites in the intestine and the muscle larvae at a dose of 500 larvae/mouse. In animals receiving the SCFA, 24 050 ± 10 415 larvae were observed in muscles, while in the infected mice, which did not receive the acids, 32 875 ± 16 762 larvae were detected (P < 0.05). An increase in the intensity of infection accelerated the rate of host reaction to the presence of T. spiralis in the intestines (self-cure). To summarize, the administered solution of short chain fatty acids alleviated the formation of histopathological changes in the intestine in response to the parasite's presence, and lowered the intensity of T. spiralis invasion after infection with a higher dose of larvae.

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Enhancement of resistance in mice treated with Lactobacillus casei: Effect on Trichinella spiralis infection

Cited by 41 publications

References 21 publications

Enhanced Antigen-Specific Delayed-Type Hypersensitivity and Immunoglobulin G2b Responses after Oral Administration of ViableLactobacillus caseiYIT9029 in Wistar and Brown Norway Rats

Enhanced Antigen-Specific Delayed-Type Hypersensitivity and Immunoglobulin G2b Responses after Oral Administration of ViableLactobacillus caseiYIT9029 in Wistar and Brown Norway Rats

The treatment of mice with Lactobacillus casei induces protection against Babesia microti infection

The influence of orally administered short chain fatty acids on intestinal histopathological changes and intensity of Trichinella spiralis infection in mice

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