Enhanced Antigen-Specific Delayed-Type Hypersensitivity and Immunoglobulin G2b Responses after Oral Administration of ViableLactobacillus caseiYIT9029 in Wistar and Brown Norway Rats

Abstract

In this study, the effects of orally administered viable Lactobacillus casei Shirota strain YIT9029 on the immunity parameters of Wistar and Brown Norway rats were examined. For this purpose, we used the Trichinella spiralis host resistance model. Two weeks before and during T. spiralis infection, rats were fed 10 9 viable L. casei bacteria 5 days per week. The T. spiralis-specific delayed-type hypersensitivity (DTH) response was significantly enhanced in both Wistar and Brown Norway rats given L. casei. In both rat strains fed L. casei, serum T. spiralis-specific immunoglobulin G2b (IgG2b) concentrations were also significantly increased. In the model, no significant effects of L. casei on larval counts or inflammatory reactions in the tongue musculature, body weights, or lymphoid organ weights were observed. Serum specific antibody responses, other than IgG2b, were not changed by feeding of L. casei. In contrast to L. casei, it was shown that orally administered Bifidobacterium breve or Bifidobacterium bifidum had no influence on the measured infection and immunity indices in the rat infection model. Since the rat DTH response is considered to be a manifestation of Th1 cell-mediated immunity and the IgG2b isotype has been associated with Th1 activity, it was concluded that Th1 cells could play an active role in the immunomodulatory effects of orally administered L. casei. Furthermore, our data do not indicate that the effect of oral supplementation with L. casei is dependent on the genetic background of the host.

Suppressive Effect on Activation of Macrophages by Lactobacillus casei Strain Shirota Genes Determining the Synthesis of Cell Wall-Associated Polysaccharides

“…There are several reports that indicate host immune responses to lactic acid bacteria and bifidobacteria, in which the involvement of various surface components of these bacteria are demonstrated (8,12,23,25,33,43). Lactobacillus casei Shirota is one of the pioneer strains of probiotics, whose immune modulation activities have been studied extensively (9,17,23,24,35), and the contributions of lipoteichoic acid (23) and polysaccharide-peptidoglycan (PS-PG) complex (24) on its cell surface to immune stimulation and immune suppression activities have been suggested. However, the role and the function of cellular components often change their intrinsic functional properties once they are extracted from the original positions.…”

Effect of spray application ofLactobacillus plantarumonin vivoperformance, caecal fermentations and haematological traits of suckling rabbits

“…Probiotic Lactobacillus are known to confirm various health promoting activities on their host after either parenteral or oral administration in rats (de Waard et al, 2001;Oyetayo et al, 2003). Some of their beneficial effects include prevention of intestinal infection (Tannock, 1983;Casas and Dobrogosz, 2000), control of serum cholesterol (Bertazzoni et al, 2001), enhancement of immunity (Aattouri et al, 2001) in human and rats, and growth enhancement of poultry and pigs (Baird, 1977;Chang et al, 2001).…”

“…Some of their beneficial effects include prevention of intestinal infection (Tannock, 1983;Casas and Dobrogosz, 2000), control of serum cholesterol (Bertazzoni et al, 2001), enhancement of immunity (Aattouri et al, 2001) in human and rats, and growth enhancement of poultry and pigs (Baird, 1977;Chang et al, 2001). The mechanisms by which these probiotics affect their host and improve gut barrier can be due to: competition for adhesion site, production of inhibitory compounds, and rebalancing of disturbed gastrointestinal microbial composition and metabolism (de Waard et al, 2001;FAO/WHO, 2001). Lactobacillus are not regular inhabitants of the digestive tract in rabbits and, according to some authors (Maertens et al, 2006), poorly adhere to epithelial cells; therefore, their usefulness is doubtful in such species (Yu and Tsen, 1993).…”

Probiotics: Immunomodulation and Evaluation of Safety and Efficacy

“…1 The most commonly used probiotics are lactic acid bacteria, mainly Lactobacillus and Bifidobacterium strains. Health effects that are ascribed to the intake of probiotics include beneficial effects on disease resistance, [2][3][4][5][6] gastrointestinal diseases, [7][8][9] and allergies. [10][11][12] Mechanisms underlying these beneficial effects are not completely understood, so the effects of probiotics should not be generalized, because different strains can exert different effects.…”