Enhancement of radiation effect and increase of apoptosis in lung cancer cells by thio-glucose-bound gold nanoparticles at megavoltage radiation energies

Wang, Cuihong; Li, Xiaohong; Wang, Yan; Liu, Zhen; Fu, Lei; Hu, Likuan

doi:10.1007/s11051-013-1642-1

Cited by 55 publications

(41 citation statements)

References 28 publications

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“…This consequently results in the retention of the mNPs within cancer cells [6]. It was proposed that gold nanoparticles (GNPs) (1–1,000 nm) modified by glucose have been considered to increase the toxicity of radiotherapy in human malignant cells [22]. There are other reports indicating that thiolamines enhance the radiation-induced apoptosis [23] We note that 2-mercaptoethyl derivative of FDG conjugated-Fe3O4 NPs has less apoptotic effect comparing to 2-mercaptoethyl derivative of FDG.…”

Section: Resultsmentioning

confidence: 99%

A Pilot Study Into the Use of FDG-mNP as an Alternative Approach in Neuroblastoma Cell Hyperthermia

Subramanian

Pearce

Guldu

et al. 2016

IEEE Trans.on Nanobioscience

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Purpose Herein, we present a pilot study concerning the use of fluorodeoxy glucose conjugated magnetite nanoparticles as a potential agent in magnetic nanoparticle mediated neuroblastoma cancer cell hyperthermia. This approach makes use of the ‘Warburg effect’, utilising the fact that cancer cells have a higher metabolic rate than normal cells. Materials and methods FDG-mNP were synthesized, then applied to the SH-SY5Y neuroblastoma cancer cell line and exposed to an AC magnetic field. 3D Calorimetry was performed on the FDG-mNP compound. Simulations were performed using SEMCAD X software using Thelonious, (an anatomically correct male child model) in order to understand more about the end requirements with respect to cancer cell destruction. Results We investigated FDG-mNP mediated neuroblastoma cytotoxicity in conjunction with AC magnetic field exposure. Results are presented for 3D FDG-mNP SARmnp (10.86 ± 0.99 W/g of particles) using a therapeutic dose of 0.83 mg/mL. Human model simulations suggest that 43 W/kg SARTheo would be required to obtain 42 °C within the centre of a liver tumour (Tumour size, bounding box x=64, y=61, z=65 [mm]), and that the temperature distribution is inhomogeneous within the tumour. Conclusion Our study suggests that this approach could potentially be used to increase the temperature within cells that would result in cancer cell death due to hyperthermia. Further development of this research will also involve using whole tumours removed from living organisms in conjunction with magnetic resonance imaging and positron emission tomography.

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Section: Resultsmentioning

confidence: 99%

A Pilot Study Into the Use of FDG-mNP as an Alternative Approach in Neuroblastoma Cell Hyperthermia

Subramanian

Pearce

Guldu

et al. 2016

IEEE Trans.on Nanobioscience

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“…The first one was the cell redistribution, as a consequence of NP exposure, into a radiosensitive phase of cell cycle such as G 2 /M. Indeed, for gold NP, several groups reported such an increase in G2/M cells for different cell lines (DU-145 (Roa et al, 2009), MDA-MB-231 , SK-OV-3 (Geng et al, 2011), HepG2 (Zhu et al, 2015), A549 (Wang et al, 2013). The NP used have in common a medium size ( > 10 nm in diameter) and a coating containing sugar (thioglucose or galactose).…”

Section: Is There a "Biological" Effect?mentioning

confidence: 99%

Actual questions raised by nanoparticle radiosensitization

Brun

Sicard-Roselli

2016

Radiation Physics and Chemistry

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“…DOX is a broad-spectrum anti-cancer drug and was reported to cure many kinds of cancers, including numerous reports for melanoma and hepatic carcinoma (13,(35)(36)(37). The cellular uptake results presented that Au concentration decreased after reaching the peak concentration, which could be explained by the exocytosis of AuNPs in the later stage (38). For biocompatibility, the AuNPs were coated with PEG which is extensively used to improve the stability in biological media (8).…”

Section: Discussionmentioning

confidence: 99%

“…These could be explained by the different radiosensitivities of different cells and by the different cellular uptake rates of AuNPs on different cells (48.5% for B16, 28.9% for HepG2 at 24 h) (4,29,39). Previous studies on cell viability were mostly carried out with X-ray dose constant or changed without a gradation (38). This study, however, gave more details on the relationship of sensitization activity not only with AuNP concentrations but also with the X-ray doses, offering more information in understanding the radiosensitization mechanism.…”

Section: Discussionmentioning

confidence: 99%

Thermosensitive Hydrogel Co-loaded with Gold Nanoparticles and Doxorubicin for Effective Chemoradiotherapy

Zhang

Wang

et al. 2015

AAPS J

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Abstract. Chemoradiotherapy, as a well-established paradigm to treat various cancers, still calls for novel strategies. Recently, gold nanoparticles (AuNPs) have been shown to play an important role as a radiosensitizer in cancer radiotherapy. The aim of this study was to evaluate the combination of polyethylene glycol (PEG) modified AuNPs and doxorubicin (DOX) to improve cancer chemoradiotherapy, in which the AuNPs was the radiosensitizer and the DOX was the model chemotherapeutic. A Pluronic® F127-based thermosensitive hydrogel (Au-DOX-Gel) loading AuNPs and DOX was developed by Bcold method^for intratumoral injection. The formulation was optimized at a F127 concentration of 22% for Au-DOX-Gel. The release profiles compared to a control group were assessed in vitro and in vivo. Au-DOX-Gel showed sustained release of AuNPs and DOX. The cell viability and surviving fraction of mouse melanoma (B16) and Human hepatocellular liver carcinoma (HepG2) cells were significantly inhibited by the combination treatment of DOX and AuNPs under radiation. Tumor sizes of mice were significantly decreased by Au-DOX-Gel compared to controls. Interestingly, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and Ki-67 staining results showed that tumor cell growth and proliferation were inhibited by AuNPs combined with DOX under radiation, suggesting that the radiosensitization activity and combination effects might be caused by inhibition of tumor cell growth and proliferation. Furthermore, the results of skin safety tests, histological observation of organs, and the body weight changes indicated in vivo safety of Au-DOX-Gel. In conclusion, the Au-DOX-Gel developed in this study could represent a promising strategy for improved cancer chemoradiotherapy.

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Enhancement of radiation effect and increase of apoptosis in lung cancer cells by thio-glucose-bound gold nanoparticles at megavoltage radiation energies

Cited by 55 publications

References 28 publications

A Pilot Study Into the Use of FDG-mNP as an Alternative Approach in Neuroblastoma Cell Hyperthermia

A Pilot Study Into the Use of FDG-mNP as an Alternative Approach in Neuroblastoma Cell Hyperthermia

Actual questions raised by nanoparticle radiosensitization

Thermosensitive Hydrogel Co-loaded with Gold Nanoparticles and Doxorubicin for Effective Chemoradiotherapy

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