Enhancement of polysaccharides accumulation in Dendrobium officinale by exogenously applied methyl jasmonate

Yuan, Zuoqing; Zhang, Jianyong; Liu, T.

doi:10.1007/s10535-016-0702-7

Cited by 13 publications

(7 citation statements)

References 24 publications

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“…In this study, tetraploid plantlets produced significantly higher polysaccharide contents in leaves, stems, and roots than diploid plantlets. The results suggested that young plantlets produce abundant polysaccharide, as documented by Yuan et al (2017), and that the polysaccharide contents in tetraploid plantlets were much higher than those in diploid parental plantlets. Similar results were also reported by Jiang et al (2014).…”

Section: Discussionmentioning

confidence: 90%

Changes in Morphological Characteristics, Regeneration Ability, and Polysaccharide Content in Tetraploid Dendrobium officinale

Pham¹,

Li²,

Guo³

et al. 2019

horts

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Dendrobium officinale Kimura et Migo is a famous traditional Chinese medicinal plant. It produces various phytochemicals, particularly polysaccharides, which have nutraceutical and pharmaceutical values. To increase its biomass production and polysaccharide content, our breeding program has generated a series of polyploid cultivars through colchicine treatment of protocorm-like bodies (PLBs). The present study compared two tetraploid cultivars, 201-1-T1 and 201-1-T2, with their diploid parental cultivar, 201-1, in an established in vitro culture system. Tetraploid ‘201-1-T1’ and ‘201-1-T2’ had shorter leaves and shorter and thicker stems and roots, and they produced higher biomass compared with the diploid cultivar. The length and width of stomata significantly increased, but stomatal density decreased in tetraploid cultivars. The PLB induction rates from the stem node explants of the tetraploid cultivars were significantly higher than those of diploid. However, the PLB proliferation of tetraploids was lower than that of the diploid. The mean number of plantlets regenerated from tetraploid PLBs was also lower than that of the diploid after 4 months of culture. Polysaccharide contents in stems, leaves, and roots of 6-month-old tetraploid plantlets were significantly higher than those of diploids. The polysaccharide content in the stem of ‘201-1-T1’ was 12.70%, which was a 2-fold increase compared with the diploid cultivar. Our results showed that chromosome doubling could be a viable way of improving D. officinale in biomass and polysaccharide production.

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Section: Discussionmentioning

confidence: 90%

Changes in Morphological Characteristics, Regeneration Ability, and Polysaccharide Content in Tetraploid Dendrobium officinale

Pham¹,

Li²,

Guo³

et al. 2019

horts

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“…Methyl jasmonate (MeJA) has been identified as a signaling molecule that induces gene expression and elicits secondary metabolic pathways in plant cells [ 13 ]. The effects of MeJA on the production of bioactive compounds (alkaloids, polysaccharides, and flavonoids) in D. officinale protocorm-like bodies has been reported [ 14 , 15 ]. In addition, several attempts to increase the production of TIAs by precursor feeding have been performed, such as the production of reserpine from Rauvolfia serpentina fed with tryptamine and the production of ajmalicine, vindoline and catharanthine from C. roseus fed with tryptophan, tryptamine, secologanin and loganin [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate

Chen

Song

Zheng

et al. 2018

IJMS

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Alkaloids are the main active ingredients in the medicinal plant Dendrobium officinale. Based on the published genomic and transcriptomic data, a proposed terpenoid indole alkaloid (TIA) biosynthesis pathway may be present in D. officinale. In this study, protocorm-like bodies (PLBs) with a high-yielding production of alkaloids were obtained by the optimization of tryptophan, secologanin and methyl jasmonate (MeJA) treatment. The results showed that the total alkaloid content was 2.05 times greater than that of the control group when the PLBs were fed with 9 µM tryptophan, 6 µM secologanin and 100 µM MeJA after 36 days. HPLC analysis showed that strictosidine synthase (STR) activity also increased in the treated plants. A total of 78 metabolites were identified using gas chromatography-mass spectrometry (GC-MS) in combination with liquid chromatography-mass spectrometry (LC-MS) methods; 29 differential metabolites were identified according to the multivariate statistical analysis. Among them, carapanaubine, a kind of TIA, exhibited dramatically increased levels. In addition, a possible underlying process of the metabolic flux from related metabolism to the TIA biosynthetic pathway was enhanced. These results provide a comprehensive view of the metabolic changes related to alkaloid biosynthesis, especially TIA biosynthesis, in response to tryptophan, secologanin and MeJA treatment.

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“…Jasmonate (JA) is a plant-specific signaling molecule broadly associated with the biosynthesis of various secondary metabolites. The exogenous application of methyl jasmonate (MeJA) has been frequently used to manipulate the production of polysaccharides [ 44 ] and alkaloids in D. catenatum [ 45 ]. In the present study (Fig.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterization of active ingredients related β-Glucosidases in Dendrobium catenatum

et al. 2022

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Background Dendrobium catenatum/D. officinale (here after D. catenatum), a well-known economically important traditional medicinal herb, produces a variety of bioactive metabolites including polysaccharides, alkaloids, and flavonoids with excellent pharmacological and clinical values. Although many genes associated with the biosynthesis of medicinal components have been cloned and characterized, the biosynthetic pathway, especially the downstream and regulatory pathway of major medicinal components in the herb, is far from clear. β-glucosidases (BGLUs) comprise a diverse group of enzymes that widely exist in plants and play essential functions in cell wall modification, defense response, phytohormone signaling, secondary metabolism, herbivore resistance, and scent release by hydrolyzing β-D-glycosidic bond from a carbohydrate moiety. The recent release of the chromosome-level reference genome of D. catenatum enables the characterization of gene families. Although the genome-wide analysis of the BGLU gene family has been successfully conducted in various plants, no systematic analysis is available for the D. catenatum. We previously isolated DcBGLU2 in the BGLU family as a key regulator for polysaccharide biosynthesis in D. catenatum. Yet, the exact number of DcBGLUs in the D. catenatum genome and their possible roles in bioactive compound production deserve more attention. Results To investigate the role of BGLUs in active metabolites production, 22 BGLUs (DcBGLU1-22) of the glycoside hydrolase family 1 (GH1) were identified from D. catenatum genome. Protein prediction showed that most of the DcBGLUs were acidic and phylogenetic analysis classified the family into four distinct clusters. The sequence alignments revealed several conserved motifs among the DcBGLU proteins and analyses of the putative signal peptides and N-glycosylation site revealed that the majority of DcBGLU members dually targeted to the vacuole and/or chloroplast. Organ-specific expression profiles and specific responses to MeJA and MF23 were also determined. Furthermore, four DcBGLUs were selected to test their involvement in metabolism regulation. Overexpression of DcBGLU2, 6, 8, and 13 significantly increased contents of flavonoid, reducing-polysaccharide, alkaloid and soluble-polysaccharide, respectively. Conclusion The genome-wide systematic analysis identified candidate DcBGLU genes with possible roles in medicinal metabolites production and laid a theoretical foundation for further functional characterization and molecular breeding of D. catenatum.

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Enhancement of polysaccharides accumulation in Dendrobium officinale by exogenously applied methyl jasmonate

Cited by 13 publications

References 24 publications

Changes in Morphological Characteristics, Regeneration Ability, and Polysaccharide Content in Tetraploid Dendrobium officinale

Changes in Morphological Characteristics, Regeneration Ability, and Polysaccharide Content in Tetraploid Dendrobium officinale

Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate

Genome-wide identification and characterization of active ingredients related β-Glucosidases in Dendrobium catenatum

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