The data here, in addition to prove the heterogeneity of hUC-MSCs, confirmed that the stronger therapeutic effects of hUC-MSC1 were attributed to its stronger anti-apoptotic effect, paracrine of trophic factors and potential RPE cell differentiation capacity. Thus, the subset hUC-MSC1, not the other subset or the ungrouped hUC-MSCs should be used for effective treatment of RD.
Polysaccharides from Dendrobium officinale possess unparalleled medicinal value. In order to produce the active polysaccharides from D. officinale through tissue culture, the effects of salicylic acid on the accumulation of polysaccharides were investigated. Salicylic acid (SA) was beneficial to increasing the contents of polysaccharides. The highest polysaccharide production occurred on the medium supplied with 100 μmol·L −1 salicylic acid. After 30 days of culture the production of polysaccharides reached 10.09% and 15.81% by phenol-sulfuric acid method and 3, 5-dinitrosalicylic (DNS) colorimetric method respectively. The major constituent of polysaccharides, glucose and mannose, was determined by pre-column derivatization-HPLC. The results showed that the glucose contents were reduced and mannose contents were increased with different salicylic acid concentration. The ANOVA of the mannose contents demonstrated that among the different SAs concentration was significantly different. The SA application influenced polysaccharide production rather than degradation because the sucrose metabolic enzyme activities were modified by SA. The experiments suggest that salicylic acid could be an effective compound to enhance the production of active polysaccharides from D. officinale.
The genetic relationship of 36 Dendrobium species in China was determined based on sequence analysis of the internal transcribed spacer (ITS) region of ribosomal DNA. Aligned sequences of the complete ITS region obtained from the 36 Dendrobium species and 2 outgroup species (Epigeneium amplum and Epigeneium nakaharaei) by using PCR amplification and direct DNA sequencing. The nrDNA ITS1 of Dendrobium was 225 -234 bp and ITS2 was 239 -248 bp. Phylogenetic tree was constructed, and seven main clusters were generated among the 36 Dendrobium species. From the results, D. moulmeinense was not grouped in the classification of Dendrobium and E. amplum and E. nakaharaei were shown to be divergent from Dendrobium species. The phylogenetic relationships revealed by ITS DNA analysis partially supported previously published morphological data.
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