Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate

Chen, Jiao; Song, Cheng; Zheng, Siyan; Zhu, Yingpeng; Jin, Qing; Cai, Yongping; Lin, Yi

doi:10.3390/ijms19030728

Cited by 31 publications

(33 citation statements)

References 53 publications

(61 reference statements)

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“…Many studies have con rmed that the phytohormone methyl jasmonate (MeJA) can enhance the biosynthesis of secondary metabolites in o cinal plants [19][20][21]. MeJA, as an elicitor, plays a signal role in the biosynthesis of plant alkaloids and has been reported to promote the accumulation of metabolites in Dendrobium Plants [22][23][24][25]. In the present study, TIBS and MeJA were used to promote biomass and alkaloid accumulation in D. nobile seedlings.…”

Section: Introductionmentioning

confidence: 84%

Improving large-scale biomass and total alkaloid production of Dendrobium nobile Lindl. using a temporary immersion system and MeJA

Zhang

Niu

et al. 2021

Preprint

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Background Dendrobium nobile Lindl. is an important pharmacopeial plant with medicinal and ornamental value. This study sought to provide technical means for the large-scale artificial production of total alkaloids in D. nobile. Seedlings were cultured in vitro in a temporary immersion bioreactor system (TIBS). The four tested immersion frequencies (min/h; 5/2, 5/4, 5/6, and 5/8) influenced the production of biomass and total alkaloid content. In addition, to compare the effects of different concentrations of phytohormone methyl jasmonate (MeJA) and treatment time on biomass and total alkaloid accumulation, MeJA was added to the TIBS medium after 50 days. Finally, the production of total alkaloids in a semi-solid system (SSS), TIBS, and TIBS combined with the MeJA system (TIBS-MeJA) were compared. Results The best immersion frequency was found to be 5/6 (5 min every 6 h) that ensured good levels of biomass and total alkaloid content in plantlets. 10 µM MeJA and 20 days of culture were the most favorable for alkaloid accumulation in the seedlings cultured in TIBS. The maximum content and productivity of total alkaloid on use of TIBS-MeJA was 2.32- and 4.69- fold, respectively, higher in terms of content, and 2.04- and 10.27- fold, respectively, higher in terms of productivity than those on use of TIBS and SSS. Conclusions Our results show that TIBS-MeJA is suitable for the large-scale production of total alkaloids in in vitro seedlings; therefore, they provide technical means for the large-scale artificial production of total alkaloids in D. nobile.

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Section: Introductionmentioning

confidence: 84%

Improving large-scale biomass and total alkaloid production of Dendrobium nobile Lindl. using a temporary immersion system and MeJA

Zhang

Niu

et al. 2021

Preprint

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“…The differential metabolites were selected on the basis of the combination of the statistically significant threshold of variable influence on projection (VIP) values obtained from the OPLS-DA model and the p -value from a two-tailed Student’s t -test of the normalized peak area. The VIP value means variables have influence on classification [ 35 ]. Through this analysis, whether compounds in different groups significantly contributed (VIP > 1, p < 0.05) was determined [ 17 , 36 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear

Abdullah

et al. 2018

IJMS

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To investigate the effect of pollination on the fruit quality of ‘Dangshan Su’ pear, ‘Dangshan Su’ was fertilized by the pollen of ‘Wonhwang’ (Pyrus pyrifolia Nakai.) (DW) and ‘Jingbaili’ (Pyrus ussuriensis Maxim.) (DJ). The analysis of primary metabolites was achieved through untargeted metabolomics, and the quantitative analysis of intermediate metabolites of lignin synthesis was undertaken using targeted metabolomics. The untargeted metabolomics analysis was performed via gas chromatography-mass spectrometry (GC-MS). The targeted metabolomics analysis was performed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) under the multiple reaction monitoring (MRM) mode. The results showed that the metabolite content was significantly different between DW and DJ. Compared with that in DJ, the sugar and amino acid content in DW was higher and the fatty acid content was lower at 47 days after pollination (DAPs), and the sugar, amino acid, and fatty acid content in DW was lower at 63 DAPs. The intermediate metabolites of lignin synthesis were analyzed using the orthogonal partial least squares discriminant analysis (OPLS-DA) model, and the differential metabolites at 47 DAPs were p-coumaric acid, ferulic acid, sinapaldehyde, coniferyl alcohol, and sinapyl alcohol. The differential significant metabolite at 63 DAPs was p-coumaric acid. At 47 DAPs and 63 DAPs, the p-coumaric acid level was significantly different, and the p-coumaric acid content was positively correlated with lignin synthesis. The pollination pollen affects the quality of ‘Dangshan Su’ pear fruit through regulation of the sugar, amino acid, and fatty acid content; at the same time, regulating the levels of intermediate metabolites of lignin synthesis, especially the p-coumaric acid content, to affect lignin synthesis ultimately affects the stone cell content and improves the quality of the pears.

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“…Among the Dendrobium species, D. officinale Kimura et Migo, commonly known as “Tiepi shihu”, is considered to have the best medicinal properties in traditional Chinese medicine (TCM) [ 2 ]. At least 190 compounds have been isolated from D. officinale , including polysaccharides, phenanthrenes, phenols, bibenzyls, essential oils, alkaloids, flavonoids, and several trace mineral elements [ 3 , 4 ]. Modern pharmacological studies have shown that D. officinale can enhance immunity, lower blood glucose levels, protect the liver and stomach, and has anti-tumor, anti-oxidation, anti-bacteria, and anti-inflammation functions [ 3 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and Metabolome Reveal Salt-Stress Responses of Leaf Tissues from Dendrobium officinale

Zhang

Zeng

et al. 2021

Biomolecules

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Dendrobium officinale Kimura et Migo is a precious traditional Chinese medicine. Despite D. officinale displaying a good salt-tolerance level, the yield and growth of D. officinale were impaired drastically by the increasing soil secondary salinization. The molecular mechanisms of D. officinale plants’ adaptation to salt stress are not well documented. Therefore, in the present study, D. officinale plants were treated with 250 mM NaCl. Transcriptome analysis showed that salt stress significantly altered various metabolic pathways, including phenylalanine metabolism, flavonoid biosynthesis, and α-linolenic acid metabolism, and significantly upregulated the mRNA expression levels of DoAOC, DoAOS, DoLOX2S, DoMFP, and DoOPR involved in the jasmonic acid (JA) biosynthesis pathway, as well as rutin synthesis genes involved in the flavonoid synthesis pathway. In addition, metabolomics analysis showed that salt stress induced the accumulation of some compounds in D. officinale leaves, especially flavonoids, sugars, and alkaloids, which may play an important role in salt-stress responses of leaf tissues from D. officinale. Moreover, salt stress could trigger JA biosynthesis, and JA may act as a signal molecule that promotes flavonoid biosynthesis in D. officinale leaves. To sum up, D. officinale plants adapted to salt stress by enhancing the biosynthesis of secondary metabolites.

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Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate

Cited by 31 publications

References 53 publications

Improving large-scale biomass and total alkaloid production of Dendrobium nobile Lindl. using a temporary immersion system and MeJA

Improving large-scale biomass and total alkaloid production of Dendrobium nobile Lindl. using a temporary immersion system and MeJA

Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear

Transcriptome and Metabolome Reveal Salt-Stress Responses of Leaf Tissues from Dendrobium officinale

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