Enhancement of Paclitaxel Production in Plant Cell Culture Including Increased Amount of Water-immiscible 1-Butyl-1-methylpyrrolidinium Bis(trifluoromethanesulfonyl)imide

Abstract

We report here the effect of increased amount of a water-immiscible ionic liquid (IL), 1-butyl-1-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide (P14-TFSI), enhancing the production amount of paclitaxel with in situ extraction from an aqueous medium in a plant cell culture. The productivity of paclitaxel in the culture including the IL of 10 vol% was 1.5 times greater than that of 2.5 vol%. Moreover, addition of an elicitor, methyl jasmonate (MJ) enhanced the production amount of paclitaxel in the culture including 10% P14-TFSI. The amount of paclitaxel in the culture including 1 µM MJ and 10 vol% P14-TFSI was more than 78 times greater than those in the control culture without both P14-TFSI and MJ, and two times greater than those in the culture including 2.5 vol% P14-TFSI alone.

Effective Production of Paclitaxel and the Related Taxanes in a Plant Cell Culture by<i> in situ</i> Extraction with Sequential Refreshment of Water-Immiscible 1-Butyl-1- methylpyrrolidinium Bis(trifluoromethanesulfonyl)imide

“…In previous research, increasing the amount of P14TFSI from 2.5 to 10 vol% in the two-phase culture system to reduce the PTX's feedback inhibition improved the cell growth rate and enhanced the production amount of the PTX by factor of 1.5 [19]. The productivity of PTX in the culture with twice refreshment of 2.5 vol% P14TFSI in this research is 7 times that without the refreshment (Figure 5).…”