Production of Paclitaxel in a Plant Cell Culture by<i> In Situ</i> Extraction with Sequential Refreshment of Water-Immiscible 1-Butyl-1-methylpyrrolidinium Bis(trifluoromethanesulfonyl)imide

Effect of Water-Soluble Coronatine on Production of Paclitaxel in a Plant Cell Culture with Water-immiscible 1-Butyl-1-methylpyrrolidinium Bis(trifluoromethanesulfonyl)imide

“…Oxidative burst, characterized by rapid and transient production of ROS, is an early event in the defense response by elicitors and an abiotic stress [19]. As discussed in the previous research, the trend of the ROS content was similar to that of the amount of paclitaxel produced in the cultures including P14TFSI [16].…”

“…Symes et al described that significant extraction of antioxidants from green asparagus root with an ionic liquid, 1-butyl-3-methylimidazolium chloride [15]. The enhanced production amount of paclitaxel in two-phase culture system with in situ extraction by a hydrophobic ionic liquid, 1-butyl-1-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide (P14TFSI, Figure 1) was previously reported [16].…”

“…Intracellular reactive oxygen species (ROS) content with the aid of 2',7'-dichlorofluorescein diacetate (DCFH-DA, Nacalai tesque, Japan) by modifying Kellett's method [20] was measured [16]. Briefly, a black plate, where 200 µM DCFH-DA in phosphate buffered saline (pH7.4) and the homogenized 5 mg cells collected from each culture condition were mixed, was excited at 484 nm in the Fluorometer (Fluoroskan Ascent, Thermo Scientific, Japan) and the emitted fluorescence intensity at 527 nm was measured as the ROS content.…”

“…Table 1. Properties of P14TFSI [16]. Coronatine (Figure 2) purchased from Sigma-Aldrich (USA) was used in the plant cell culture as an elicitor to enhance the productivity of paclitaxel.…”

Effective Production of Paclitaxel and the Related Taxanes in a Plant Cell Culture by<i> in situ</i> Extraction with Sequential Refreshment of Water-Immiscible 1-Butyl-1- methylpyrrolidinium Bis(trifluoromethanesulfonyl)imide

“…It is reported amino acid ester based phenolic ionic liquids can be a potential solvent for poorly soluble bioactive compound luteolin [16]. We previously reported the enhanced production amount of paclitaxel in plant cell culture with the in situ extraction by sequential refreshment of a hydrophobic ionic liquid, 1-butyl-1-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide (P14TFSI) [17].…”