Enhancement of Microbubble Mediated Gene Delivery by Simultaneous Exposure to Ultrasonic and Magnetic Fields

Stride, Eleanor; Porter, Colin D.; Prieto, A.; Pankhurst, Quentin A.

doi:10.1016/j.ultrasmedbio.2008.11.010

Cited by 93 publications

(75 citation statements)

References 16 publications

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“…This study shows that DSPC tMBs keep their spherical shapes after binding ( Figure 1B1) and have significantly smaller binding areas than DPPC tMBs, which had a dome shape after binding ( Figure 1B2). Magnetic MBs have also been developed to enhance the targeting in UMI and therapy [53][54][55]. Magnetic targeting uses an externally applied magnetic field, typically applied using a permanent magnet, to control the location of magnetically responsive MBs.…”

Section: Techniques To Enhance Bindingmentioning

confidence: 99%

“…Magnetic targeting uses an externally applied magnetic field, typically applied using a permanent magnet, to control the location of magnetically responsive MBs. Various magnetic MB preparations and applications have been published by Stride et al in 2009 and2012 [54,56]. For instance, the gas core of the MB can be surrounded by a ferrofluid and stabilised by an outer coating of L-a-PC.…”

Section: Techniques To Enhance Bindingmentioning

confidence: 99%

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Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Rooij

Daeichin

Skachkov

et al. 2015

International Journal of Hyperthermia

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Ultrasound contrast agents (UCAs) are used routinely in the clinic to enhance contrast in ultrasonography. More recently, UCAs have been functionalised by conjugating ligands to their surface to target specific biomarkers of a disease or a disease process. These targeted UCAs (tUCAs) are used for a wide range of pre-clinical applications including diagnosis, monitoring of drug treatment, and therapy. In this review, recent achievements with tUCAs in the field of molecular imaging, evaluation of therapy, drug delivery, and therapeutic applications are discussed. We present the different coating materials and aspects that have to be considered when manufacturing tUCAs. Next to tUCA design and the choice of ligands for specific biomarkers, additional techniques are discussed that are applied to improve binding of the tUCAs to their target and to quantify the strength of this bond. As imaging techniques rely on the specific behaviour of tUCAs in an ultrasound field, it is crucial to understand the characteristics of both free and adhered tUCAs. To image and quantify the adhered tUCAs, the state-of-the-art techniques used for ultrasound molecular imaging and quantification are presented. This review concludes with the potential of tUCAs for drug delivery and therapeutic applications.

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Section: Techniques To Enhance Bindingmentioning

confidence: 99%

Section: Techniques To Enhance Bindingmentioning

confidence: 99%

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Rooij

Daeichin

Skachkov

et al. 2015

International Journal of Hyperthermia

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show abstract

“…The transducer featured a rectangular cutout through which an imaging linear array (model L11-4v; Verasonics Inc., USA) was aligned. [25] Samples were exposed to 10 s of ultrasound (pulse center frequency of 500 kHz, 1 MPa peak-to-peak focal pressure, 40 cycles per burst, 1 kHz pulse repetition frequency [26] ) at five points 3 mm apart, while the imaging array passively recorded the acoustic www.advhealthmat.de www.advancedsciencenews.com emissions from every 10th burst for the first second of each exposure (100 frames, 128 channels, 170 µs per run) to an ultrasound research platform (Verasonics V1; Verasonics Inc., USA) for subsequent analysis. After ultrasound exposure, cells were further incubated at 37 °C for 24 h. The number of viable cells was determined using the MTS colorimetric assay.…”

Section: Methodsmentioning

confidence: 99%

Ultrasound‐Enhanced siRNA Delivery Using Magnetic Nanoparticle‐Loaded Chitosan‐Deoxycholic Acid Nanodroplets

Lee

Crake

Teo

et al. 2017

Adv Healthcare Materials

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Small interfering RNA (siRNA) has significant therapeutic potential but its clinical translation has been severely inhibited by a lack of effective delivery strategies. Previous work has demonstrated that perfluorocarbon nanodroplets loaded with magnetic nanoparticles can facilitate the intracellular delivery of a conventional chemotherapeutic drug. The aim of this study is to determine whether a similar agent can provide a means of delivering siRNA, enabling efficient transfection without degradation of the molecule. Chitosandeoxycholic acid nanoparticles containing perfluoropentane and iron oxide (d 0 = 7.5 ± 0.35 nm) with a mean hydrodynamic diameter of 257.6 ± 10.9 nm are produced. siRNA (AllStars Hs cell death siRNA) is electrostatically bound to the particle surface and delivery to lung cancer cells and breast cancer cells is investigated with and without ultrasound exposure (500 kHz, 1 MPa peakto-peak focal pressure, 40 cycles per burst, 1 kHz pulse repetition frequency, 10 s duration). The results show that siRNA functionality is not impaired by the treatment protocol and that the nanodroplets are able to successfully promote siRNA uptake, leading to significant apoptosis (52.4%) 72 h after ultrasound treatment.

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“…In terms of overcoming these inconveniences, magnetofection provides an alternative way of targeted gene delivery using magnetic fields. As a result, many studies related to gene delivery via magnetofection are now available in the literature [1][2][3][4][5][6][7][8][9][10][11]. For example, Scherer et al…”

Section: Introductionmentioning

confidence: 99%

“…Namiki et al [7] showed a new nanoparticle formulation composed of oleic acid-coated magnetic nanocrystal core and a cationic lipid shell, which can be magnetically guided to deliver genes more efficiently in cells and tumors. Stride et al [8] investigated the effect of both ultrasonically and magnetically-mediated DNA transfection of Chinese hamster ovary (CHO) cells. They compared in vitro uptake of DNA by cells exposed to ultrasound and/or a magnetic field in the presence of phospholipid coated microbubbles prepared with or without a suspension of magnetic nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Effect of Varying Magnetic Fields on Targeted Gene Delivery of Nucleic Acid-Based Molecules

et al. 2015

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The importance of high transfection efficiency has been emphasized in many studies investigating methods to improve gene delivery. Accordingly, non-viral transfection agents are widely used as transfection vectors to condense oligonucleotides, DNA, RNA, siRNA, deliver into the cell, and release the cargo. Polyethyleneimine (PEI) is one of the most popular non-viral transfection agents. However, the challenge between high transfection efficiency and toxicity of the polymers is not totally resolved. The delivery of necessary drugs and genes for patients and their transport under safe conditions require carefully designed and controlled delivery systems and constitute a critical stage of patients' treatment. Compact systems are considered as the strongest candidate for the preparation and delivery of drugs and genes under leak free and safe conditions because of their low energy consumption, low waste disposal, parallel and fast processing capabilities, removal of human factor, high mixing capabilities, enhanced safety, and low amount of reagents. Motivated by this need in the literature, a platform for gene delivery via magnetic actuation of nanoparticles was developed in this study. The use of PEI-SPION (Super paramagnetic ironoxide nanoparticles) as transfection agents in in vitro studies was investigated with the effect of varying magnetic fields provided by a special magnetic system design, which was used as magnetic actuator offering different magnet's turn speeds and directions in the system. Results obtained from magnetic actuator systems were compared to the experiments without actuation and significant enhancement was observed in the transfection efficacies.

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Enhancement of Microbubble Mediated Gene Delivery by Simultaneous Exposure to Ultrasonic and Magnetic Fields

Cited by 93 publications

References 16 publications

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Ultrasound‐Enhanced siRNA Delivery Using Magnetic Nanoparticle‐Loaded Chitosan‐Deoxycholic Acid Nanodroplets

Effect of Varying Magnetic Fields on Targeted Gene Delivery of Nucleic Acid-Based Molecules

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