Enhancement of maternal recognition of pregnancy with parthenogenetic embryos in bovine embryo transfer

Hirayama, Hiroki; Moriyasu, Satoru; Kageyama, Soichi; Sawai, Ken; Takahashi, Hitomi; Geshi, Masaya; Fujii, Takashi; Koyama, Takeshi; Koyama, Keisuke; Miyamoto, Akio; Matsui, Motozumi; Minamihashi, Akira

doi:10.1016/j.theriogenology.2014.01.039

Cited by 15 publications

(14 citation statements)

References 37 publications

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“…To determine whether genes that were stimulated in vitro by roIFNT were also upregulated during early pregnancy in vivo , CL were collected from pregnant cows on day 18 after AI, when IFNT concentrations are expected to be elevated 14,22 or in cyclic non-inseminated cows on day 18 of the estrous cycle. Plasma progesterone was not different between pregnant (5.97 ± 0.79 ng/ml, n = 6) and cyclic (5.81 ± 1.10 ng/ml, n = 6) cows, confirming that CL were not undergoing regression at the time of collection.…”

Section: Resultsmentioning

confidence: 99%

“…The primary distinguishing feature between IFNT and most other type I IFNs is its unique, trophoblast-specific expression pattern ruminants. In cows, IFNT is secreted by the trophoblastic cells throughout days 10–24 of pregnancy 14,22 . The presence of elevated levels of ISGs in CL of pregnant animals suggested extra-uterine, endocrine effects of IFNT 16,18,23,24 .…”

Section: Introductionmentioning

confidence: 99%

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Interferon-Tau Exerts Direct Prosurvival and Antiapoptotic Actions in Luteinized Bovine Granulosa Cells

Basavaraja

Madusanka

Drum

et al. 2019

Sci Rep

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Interferon-tau (IFNT), serves as a signal to maintain the corpus luteum (CL) during early pregnancy in domestic ruminants. We investigated here whether IFNT directly affects the function of luteinized bovine granulosa cells (LGCs), a model for large-luteal cells. Recombinant ovine IFNT (roIFNT) induced the IFN-stimulated genes (ISGs; MX2, ISG15, and OAS1Y). IFNT induced a rapid and transient (15–45 min) phosphorylation of STAT1, while total STAT1 protein was higher only after 24 h. IFNT treatment elevated viable LGCs numbers and decreased dead/apoptotic cell counts. Consistent with these effects on cell viability, IFNT upregulated cell survival proteins (MCL1, BCL-xL, and XIAP) and reduced the levels of gamma-H2AX, cleaved caspase-3, and thrombospondin-2 (THBS2) implicated in apoptosis. Notably, IFNT reversed the actions of THBS1 on cell viability, XIAP, and cleaved caspase-3. Furthermore, roIFNT stimulated proangiogenic genes, including FGF2, PDGFB, and PDGFAR. Corroborating the in vitro observations, CL collected from day 18 pregnant cows comprised higher ISGs together with elevated FGF2, PDGFB, and XIAP, compared with CL derived from day 18 cyclic cows. This study reveals that IFNT activates diverse pathways in LGCs, promoting survival and blood vessel stabilization while suppressing cell death signals. These mechanisms might contribute to CL maintenance during early pregnancy.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interferon-Tau Exerts Direct Prosurvival and Antiapoptotic Actions in Luteinized Bovine Granulosa Cells

Basavaraja

Madusanka

Drum

et al. 2019

Sci Rep

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“…Although the primary objective of this study was to use parthenogenetic embryos as the control for measuring the quality of cloning, the results produced in the group of embryos generated using parthenotes lacking the zona pellucida paves the way for future research on embryo quality, such as that reported recently (Hirayama et al, 2014). This study demonstrated that the transfer of embryos fertilized in vitro, along with parthenogenetic embryos, could aid in maternal recognition, with increased INT-tau production being observed until day 40 of gestation, in addition to generating investigations into MC transgenesis, such as that conducted in goats (Pereira et al, 2013) The in vitro production of bovine embryos has become a pioneering biotechnology for genetic improvement.…”

Section: Discussionmentioning

confidence: 99%

Handmade cloned bovine embryos, parthenogenesis and in vitro fertilization: a comparison

Rúa

Ramírez

Ruiz-Cortés

2017

RICBA

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<p>The handmade cloning and parthenogenesis as a control leads to the possibility of studying biological processes such as cell reprogramming, epigenetics, embryo genome activation and pre- and post-natal development. The objective of this study was to standardize the process the handmade cloning (HCM) and to compare the resultant embryo production with parthenogenesis and in vitro fertilization (IVF). The primary fibroblast culture was established from explants of ear, the manual cloning technique was standardized with this fibroblast and 10 processes to cloning, parthenogenesis and fertilization in vitro were performed, and the compared production and embryo quality was performed by an ANOVA. An in vitro culture of fibroblast cells was established with optimal characteristics, allowing the genetic material to be used in the process of cloning. The parthenote group without zona pellucida (ZP) exhibited a higher cleavage rate compared with the other groups of parthenotes with ZP and IVF embryo (p<0.05). The number of blastomeres was greater in the IVF (109.81±11.70) compared with the parthenote with ZP (73.73±7.09), without ZP (78.16±7.65) and clone (CL, 77.5±8.23) groups. Embryo production in the CL, ZP, without ZP and IVF groups was not significantly different (29.7, 37.6, 33.8 and 35.2% respectively). The HCM technique was successfully standardized in the laboratory. The resultant embryo production was similar between hand-made cloned embryos, parthenogenesis and in vitro fertilization. The findings of this work give rise to different routes for studying embryology and contribute to the optimization of this technique for commercial purposes.</p>

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“…It was reported successful transfer of in vivo fertilized embryos of bovine [ 8 – 10 ]. To date, most techniques of in vitro embryos production were relied on cows already slaughtered hence they are limited in a way that genetic traits of maternal bovine were not known (Rosenkrans et al, [ 11 – 13 ]; Hasler et al, [ 14 ]; Blondin et al, [ 15 , 16 ]).…”

Section: Introductionmentioning

confidence: 99%

Effects of culture media conditions on production of eggs fertilized in vitro of embryos derived from ovary of high grade Hanwoo

2016

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BackgroundThis study was investigated the effects of culture media conditions on production of eggs fertilized in vitro of embryos from ovaries of high grade Korean native cow, Hanwoo.MethodsThe IVMD 101 and IVF 100 were used for in vitro maturation of selected Hanwoo oocytes and In vitro embryo culture after in vitro fertilization, respectively. The IVMD 101 and IVD 101 were used for in vitro culture and completely free of serum.ResultsThe cleavage rates of 2-cell embryos in reference to Hanwoo oocytes were 86.7, 92.9 , and 90.1 % in the control group, IVDM101 medium and IVD101 medium, respectively which indicates that the IVDM101 medium and IVD101 medium may result favorable outcomes. The in vitro development rates of blastocysts were 12.4, 38.4 and 32.4 % in the control group, serum free IVMD101 medium and IVD101 medium, respectively. For hatched blastocysts, it was 5.3, 33.9, and 28.6 % in the control group, serum free IVMD101 medium and IVD101 medium, respectively. Hence, more favorable results were expected for the hatched blastocysts in which the IVMD101 medium and IVD101 medium were used than the control group. Average cell numbers of blastocysts were 128.3, 165.7, and 163.6 in the groups of TCM-199 + 10 % FBS medium, IVMD 101 medium, and IVD 101 medium, respectively which clearly show that the IVMD 101 and IVD 101 medium consequence significantly higher cell numbers compared to the control group (i.e., TCM-199 + 10 % FBS medium). Pregnancy rate after embryo transfer was 39.6 % when the serum free medium was used which is higher than that of the medium supplemented with serum (32.8 %). In addition, stillbirth rates were 4.9 % in the group of serum free medium whereas it was 13.6 % in the serum supplemented medium (13.6 %).ConclusionsTaken altogether, serum free media, the IVMD 101 and IVD 101 represented more favorable results in the embryo development rate of embryos, cell numbers of blastocyst, and pregnancy rate. Of note, the IVMD 101 medium showed better outcomes hence, it might be a better option for future applications for in vitro culture of bovine embryos.

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Enhancement of maternal recognition of pregnancy with parthenogenetic embryos in bovine embryo transfer

Cited by 15 publications

References 37 publications

Interferon-Tau Exerts Direct Prosurvival and Antiapoptotic Actions in Luteinized Bovine Granulosa Cells

Interferon-Tau Exerts Direct Prosurvival and Antiapoptotic Actions in Luteinized Bovine Granulosa Cells

Handmade cloned bovine embryos, parthenogenesis and in vitro fertilization: a comparison

Effects of culture media conditions on production of eggs fertilized in vitro of embryos derived from ovary of high grade Hanwoo

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