Effects of culture media conditions on production of eggs fertilized in vitro of embryos derived from ovary of high grade Hanwoo

Abstract

BackgroundThis study was investigated the effects of culture media conditions on production of eggs fertilized in vitro of embryos from ovaries of high grade Korean native cow, Hanwoo.MethodsThe IVMD 101 and IVF 100 were used for in vitro maturation of selected Hanwoo oocytes and In vitro embryo culture after in vitro fertilization, respectively. The IVMD 101 and IVD 101 were used for in vitro culture and completely free of serum.ResultsThe cleavage rates of 2-cell embryos in reference to Hanwoo oocytes were 86.7, 92.9 , and 90.1 % in the control group, IVDM101 medium and IVD101 medium, respectively which indicates that the IVDM101 medium and IVD101 medium may result favorable outcomes. The in vitro development rates of blastocysts were 12.4, 38.4 and 32.4 % in the control group, serum free IVMD101 medium and IVD101 medium, respectively. For hatched blastocysts, it was 5.3, 33.9, and 28.6 % in the control group, serum free IVMD101 medium and IVD101 medium, respectively. Hence, more favorable results were expected for the hatched blastocysts in which the IVMD101 medium and IVD101 medium were used than the control group. Average cell numbers of blastocysts were 128.3, 165.7, and 163.6 in the groups of TCM-199 + 10 % FBS medium, IVMD 101 medium, and IVD 101 medium, respectively which clearly show that the IVMD 101 and IVD 101 medium consequence significantly higher cell numbers compared to the control group (i.e., TCM-199 + 10 % FBS medium). Pregnancy rate after embryo transfer was 39.6 % when the serum free medium was used which is higher than that of the medium supplemented with serum (32.8 %). In addition, stillbirth rates were 4.9 % in the group of serum free medium whereas it was 13.6 % in the serum supplemented medium (13.6 %).ConclusionsTaken altogether, serum free media, the IVMD 101 and IVD 101 represented more favorable results in the embryo development rate of embryos, cell numbers of blastocyst, and pregnancy rate. Of note, the IVMD 101 medium showed better outcomes hence, it might be a better option for future applications for in vitro culture of bovine embryos.

Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production

“…Oocyte in vitro maturation is one of the most important steps in the in vitro production system, and it is crucial for the success of the biotechnique (Adona, Pires, Quetglas, Schwarz, & Leal, 2008;Lee, Jung, & Seo, 2016). Development rates obtained with in vitro maturation are, however, lower than those from in vivo maturation (Gilchrist, 2010).…”

“…Development rates obtained with in vitro maturation are, however, lower than those from in vivo maturation (Gilchrist, 2010). Therefore, many studies have been conducted to improve the efficiency of oocyte in vitro maturation (Adona et al, 2016;Lee et al, 2016). Several parameters, such as gaseous atmosphere, temperature, protein supplementation, growth factors and oocyte manipulation, may influence the embryo production process in vitro (Adona et al, 2008;Arat et al, 2016;Mingoti, Castro, Méo, Barretto, & Garcia, 2009).…”

Melatonin Improves the Quality of Inferior Bovine Oocytes and Promoted Their Subsequent IVF Embryo Development: Mechanisms and Results

“…The increasing importance of in vitro production of bovine embryos in commercial cattle breeding programs demands an improvement of embryo viability [ 1 ]. Oocytes in vitro maturation is one of the most important steps in the in vitro embryo production process, and it is crucial for the success of this process [ 2 , 3 ]. In many laboratories, almost half of collected cumulus-oocyte complexes (COCs) were discarded due to their poor quality.…”

<em>In vitro</em> embryos of Romosinuano and Tropical Milking cattle during three seasons in Veracruz, Mexico

“…Among the factors that affect the division of the presumed zygotes are the quality of the semen used [58], the culture medium [59] and the quality of the oocyte collected, which can come from donors under heat stress [60]. In this study, the highest means of CLI embryos were observed in the HH for RM and FD for TM and the lowest in HD also for TM with almost 70% less in relation to the highest (Table 2).…”