Enhancement of innate immune system, survival and yield inPenaeus monodonreared in ponds usingStreptococcus phocae PI80

Abstract

The objective of this study was to evaluate the effect of dietary and water supplementation of probiotic Streptococcus phocae PI80 on growth, immune response and feed utilization of tiger shrimp Penaeus monodon in earthen ponds. The probiotic bacterium S. phocae PI80 was cultured in large fermenter (50 L) by adding additional carbon source in the form of molasses and glucose along with yeast extract as nitrogen source to enrich S. phocae PI80 biomass. This enriched S. phocae PI80 was administered to shrimp in feed (6.5 × 1013 bacterial cells mL−1) as well as in pond water (5 L/pond). Shrimp growth performance was significantly improved (P < 0.05) in 120 days culture when the average body weight of treated molasses + yeast extract (MY) (28.41 ± 0.874 g), glucose + yeast extract (GY) (27.013 ± 0.698 g) was significantly higher than control (23.63 ± 0.684 g). Food conversion ratio FCR was also found to be reduced significantly in ponds treated with probiotics when compared with control pond (1.89 ± 0.09). Vibrio and luminescent bacteria were found to be lower in the treatment receiving MY group, and we hypothesize that this may lead to greater shrimp survival. Furthermore, fermentation product of S. phocae PI80 added to pond water and feed additives enhanced the shrimp immune system. The results indicated that total haemocytes count (THC), phenoloxidase (PO) activity, NBT reductase assay and phagocytic activity significantly increased in shrimps treated with S. phocae PI80. Our study demonstrates that administration of S. phocae PI80 in the water and feed at 6.5 × 1013 colony‐forming units (CFU) mL−1 bacterial cells induce immune modulation and enhances the immune ability of P. monodon in pond‐reared shrimp and increased the shrimp production.

The effects of water additive Bacillus cereus and Pediococcus acidilactici on water quality, growth performances, economic benefits, immunohematology and bacterial flora of whiteleg shrimp (Penaeus vannamei Boone, 1931) reared in earthen ponds

“…Among different probiotics, Bacillus spp. is one of the most common one so that many works have showed the positive effects of supplementing them on water quality, growth performance, immunity and controlling bacteria in shrimp (Kuebutornye, Abarike, & Lu, 2019;Pattukumar et al, 2014;Soltani et al, 2019;Zokaeifar et al, 2014). Specially, some studies investigated the effect of adding Bacillus spp.…”

Effect of commercial probiotic (Protexin®) on growth, survival and microbial quality of shrimp (Litopenaeus vannamei)

“…The enhancement in growth performance in the present study may be explained by enhanced digestion and nutrient absorption by the probiotic bacteria. They can improve the nutrition by detoxifying potentially harmful compounds in feeds, denaturing potentially indigestible components in the diet (by hydrolytic enzymes including amylases and proteases), producing vitamins such as biotin and vitamin B12, producing inhibitory compounds and stimulating the host immunity (EL-Haroun et al, 2006;Kongnum and Hongpattarakere, 2012;Pattukumar et al, 2014). Probiotic bacteria can also increase digestibility and absorption of food by enhancing the population of beneficial microorganisms, microbial enzyme activity and improving the intestinal microbial balance which leads to increased growth performance (EL-Haroun et al, 2006;Sapcharoen and Rengpipat, 2013;Karthik et al, 2014;Pattukumar et al, 2014).…”

Community structure of lactic acid producing bacteria in the guts of freshwater shrimps

“…pathogen in term of better post-infection survival (Maeda et al, 2014). Streptococcus phocae PI80 could enhance the immune ability of Penaeus monodon and increase the shrimp production while it was in the pond water and feed (Pattukumar et al, 2014). Enterococcus faecium MC13, isolated from fish intestine, could abundantly secrete bacteriocin, a synthetic peptide/protein displaying a broad spectrum of antimicrobial activity against pathogenic bacteria (Kanmani, 2011).…”