Enhancement of growth and polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense by the addition of putrescine

Wei, Mei; Jiang, Shaotong; Luo, Jian‐Ping

doi:10.1007/s10529-006-9248-7

Cited by 9 publications

(4 citation statements)

References 9 publications

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“…Protocorms and PLBs induced as above were collected as explants to be cut into pieces of approximately 0.5 cm × 0.5 cm to proliferate more PBLs, and the optimum culture protocol for proliferation of D. officinale PLBs was screened. An L 16 (4 5 ) orthogonal experiment without regarding interactions among factors was performed; the factors and their levels were selected on the basis of previous experiments and reports [51]. Four different concentrations of MS medium were used as test media (Factor A): 1/4MS, 1/2MS, MS and 2MS.…”

Section: Induction and Propagation Of D Officinale Protocorms And Plbsmentioning

confidence: 99%

Putative genes in alkaloid biosynthesis identified in Dendrobium officinale by correlating the contents of major bioactive metabolites with genes expression between Protocorm-like bodies and leaves

et al. 2021

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Background Dendrobium officinale, an endangered Chinese herb, possesses extensive therapeutic effects and contains bioactive ingredients such as major polysaccharides, alkaloids, and minimal flavonoids. We first obtained the protocorm-like bodies (PLBs) of this plant through tissue culture in order to determine the distribution of the main secondary metabolites in each organelle and the PLBs. We then analyzed the correlation between gene expression level from comparative transcriptome sequencing and metabolite content in different organs to identify putative genes encoding enzymes involved in the biosynthesis of polysaccharides, alkaloids, and flavonoids. Results We used seeds as explants for protocorm induction and PLB propagation of D. officinale. The optimal medium formula for PLB propagation was 1/2 MS + α-NAA 0.5 mg·L− 1 + 6-BA 1.0 mg·L− 1 + 2, 4-D 1.5–2.0 mg·L− 1 + potato juice 100 g·L− 1. Stems, PLBs and leaves of D. officinale had the highest content of polysaccharides, alkaloids and flavonoids, respectively. Naringenin was only produced in stem; however, PLBs with high alkaloid content can replace other organs producing alkaloids. The hot water extraction method outperformed the ultrasound-assisted extraction method for extracting polysaccharides from D. officinale. A comparative transcriptome analysis of PLBs and leaves of D. officinale revealed differential expression of genes encoding enzymes involved in polysaccharide, alkaloid and flavonoid biosynthetic pathways. Putative genes encoding enzymes involved in these biosynthetic pathways were identified. Notably, we identified genes encoding the alkaloid biosynthesis enzymes strictosidine β-D-Glucosidase, geissoschizine synthase and vinorine synthase in D. officinale. Conclusions The identification of candidate genes encoding enzymes involved in metabolite biosynthesis will help to explore and protect this endangered species and facilitate further analysis of the molecular mechanism of secondary metabolite biosynthesis in D. officinale.

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Section: Induction and Propagation Of D Officinale Protocorms And Plbsmentioning

confidence: 99%

Putative genes in alkaloid biosynthesis identified in Dendrobium officinale by correlating the contents of major bioactive metabolites with genes expression between Protocorm-like bodies and leaves

et al. 2021

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“…65 In recent years, many studies have indicated that for D. huoshanense and D. officinale, an increase in the polysaccharide content was always accompanied by the activation of SUS. [66][67][68] Therefore, in D. nobile, SUS may play the same role in polysaccharide biosynthesis is in these related plant species. This conjecture was supported by the 68.06-fold higher expression of SUS over that in the controls in the 9 th week.…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of polysaccharide accumulation in Dendrobium nobile infected with the mycorrhizal fungus Mycena sp.

Zhou

et al. 2017

RSC Adv.

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“…The main bioactive molecules are polysaccharides and alkaloids. Since it is highly valuable but rare, strategies have been developed to enhance its growth and increases in polysaccharides production [29]. Using crude extracts of polysaccharides isolated from DH, we previously showed the in vitro induction of G-CSF and a few other cytokines in mouse splenocytes [30].…”

Section: Discussionmentioning

confidence: 99%

The Multifaceted Effects of Polysaccharides Isolated from Dendrobium huoshanense on Immune Functions with the Induction of Interleukin-1 Receptor Antagonist (IL-1ra) in Monocytes

et al. 2014

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Dendrobium huoshanense is a valuable and versatile Chinese herbal medicine with the anecdotal claims of cancer prevention and anti-inflammation. However, its immunological activities are limited to in vitro studies on a few cytokines and immune cell functions. First, we investigated the effects of polysaccharides isolated from DH (DH-PS) on inducing a panel of cytokines/chemokines in mice in vivo and human in vitro. We found that DH polysaccharides (DH-PS) induced TH1, TH2, inflammatory cytokines and chemokines in mouse in vivo and human cells in vitro. Secondly, we demonstrated that DH-PS expanded mouse splenocytes in vivo including CD4+ T cells, CD8+ T cells, B cells, NK cells, NKT cells, monocytes/macrophages, granulocytes and regulatory T cells. Notably, DH-PS induced an anti-inflammatory molecule, IL-1ra, in mouse and human immune cells, especially monocytes. The serum level of IL-1ra elicited by the injection of DH-PS was over 10 folds of IL-1β, suggesting that DH-PS-induced anti-inflammatory activities might over-ride the inflammatory ones mediated by IL-1β. The signaling pathways of DH-PS-induced IL-1ra production was shown to involve ERK/ELK, p38 MAPK, PI3K and NFκB. Finally, we observed that IL-1ra level induced by DH-PS was significantly higher than that by F3, a polysaccharide extract isolated from another popular Chinese herbal medicine, Ganoderma lucidum. These results indicated that DH-PS might have potential applications for ameliorating IL-1-induced pathogenic conditions.

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Enhancement of growth and polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense by the addition of putrescine

Cited by 9 publications

References 9 publications

Putative genes in alkaloid biosynthesis identified in Dendrobium officinale by correlating the contents of major bioactive metabolites with genes expression between Protocorm-like bodies and leaves

Putative genes in alkaloid biosynthesis identified in Dendrobium officinale by correlating the contents of major bioactive metabolites with genes expression between Protocorm-like bodies and leaves

Molecular analysis of polysaccharide accumulation in Dendrobium nobile infected with the mycorrhizal fungus Mycena sp.

The Multifaceted Effects of Polysaccharides Isolated from Dendrobium huoshanense on Immune Functions with the Induction of Interleukin-1 Receptor Antagonist (IL-1ra) in Monocytes

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